公开(公告)号：DK1930419T3

公开(公告)日：2010-12-20

申请号：DK06810966

申请日：2006-09-25

Applicant: KAO CORP

Inventor： SATO HITOSHI ,  SHOGA YUTAKA ,  KOYAMA SHINGO ,  SHIRAKURA IYORI

IPC: C12N9/00

公开(公告)号：DE602006018034D1

公开(公告)日：2010-12-16

申请号：DE602006018034

申请日：2006-09-25

Applicant: KAO CORP

Inventor： SATO HITOSHI ,  SHOGA YUTAKA ,  KOYAMA SHINGO ,  SHIRAKURA IYORI

IPC: C12N9/00

公开(公告)号：EP1930419A4

公开(公告)日：2009-03-25

申请号：EP06810966

申请日：2006-09-25

Applicant: KAO CORP

Inventor： SATO HITOSHI ,  SHOGA YUTAKA ,  KOYAMA SHINGO ,  SHIRAKURA IYORI

IPC: C12N9/00

CPC classification number: C12N9/00 ,  C07K1/34 ,  C12N1/02

Abstract: A method for collecting an enzyme from an enzyme-containing solution having a cell density of 1% (v/v) or less and an enzyme concentration of 1% (w/v) or more in terms of the amount of a protein by microfiltration, wherein a cationic surfactant is added to the enzyme-containing solution in an amount of 0.01 to 1% (w/v) and the mixture is then subjected to microfiltration.