公开(公告)号：WO2020058967A1

公开(公告)日：2020-03-26

申请号：PCT/IB2019/058058

申请日：2019-09-23

Applicant: KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： ZAHER, Manal S. ,  RASHID, Fahad ,  RADUCANU, Vlad-Stefan ,  HAMDAN, Samir M.

IPC: C12N15/115 ,  G01N33/542 ,  G01N33/58

Abstract: Potassium ion sensing aptamers are disclosed. These aptamers have high specificity towards the potassium ion. Ligand-sensing aptamers with a built-in reporter are also disclosed. The built-in reporter is incorporated into the sugar-phosphate backbone of the aptamers. The built-in reporter may be an environmentally sensitive fluorescence dye, internally coupled to the aptamers. The environmentally sensitive fluorescence dye can sense the conformation changes induced by binding of the aptamers to the target ligand and transduces the conformational changes to a fluorescence change.

公开(公告)号：WO2022018685A1

公开(公告)日：2022-01-27

申请号：PCT/IB2021/056646

申请日：2021-07-22

Applicant: KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： HAMDAN, Samir M. ,  TAKAHASHI, Masateru ,  TEHSEEN, Muhammad ,  TAKAHASHI, Etsuko

IPC: C12Q1/70 ,  C12Q1/686

Abstract: Compositions and methods for a nucleic acid amplification and diagnostic methods based therein, are disclosed. The compositions include purified Thermus aquaticus DNA polymerase (Taq Pol) and Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT), in proportions and under reaction conditions that allow one-step amplification of a nucleic acid of interest. In particular, the compositions are useful in one-step RT-qPCR and RT-LAMP methods. The method includes sampling the specimen from a subject, extracting and purifying RNA from the sample. The compositions and methods may be used to produce, analyze, quantitate, detect and otherwise manipulate nucleic acid molecules. For example, disclosed are methods of detecting the presence of a viral nucleic acid in a sample from a subject. Methods of diagnosing a subject as being infected with a viral pathogen are also provided. A preferred virus or pathogen is SARS-CoV-2.

公开(公告)号：WO2020129027A1

公开(公告)日：2020-06-25

申请号：PCT/IB2019/061232

申请日：2019-12-20

Applicant: KING ABDULLAH UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： RADUCANU, Vlad-Stefan ,  TEHSEEN, Muhammad ,  RASHID, Fahad ,  HAMDAN, Samir M.

IPC: C07K1/22 ,  C07K14/47

Abstract: Described are affinity purification system that includes a carrier/surface that is non-cellular, and sliding clamp (SC) protein, and methods for purifying proteins that bind to the SC. The SC is associated with the carrier/surface via covalent/non-covalent interactions.To attain control of coupling site, the SC can be mutated via site-directed mutagenesis to introduce an exogenous residue and, the exogenous internal residue is conjugated to the non-cellular surface through the linker. The SC can also be coupled to the carrier via non-covalent interactions such as the affinity interactions involved in ligand/binding partner complex formation. The SC-based affinity purification system are used in a purification column as bait proteins, to isolate SC binding partners or non SC-binding proteins engineered to contain a SC binding site prior to its purification.

公开(公告)号：EP3129475B1

公开(公告)日：2018-07-11

申请号：EP15759935.8

申请日：2015-04-10

Applicant: King Abdullah University of Science and Technology

Inventor： HAMDAN, Samir M. ,  TAKAHASHI, Masateru

IPC: C12N9/12 ,  C12Q1/68 ,  C12P19/34

CPC classification number: C12N9/1252 ,  C12P19/34 ,  C12Y207/07007

Abstract: A DNA polymerase composition for amplifying nucleic acids can be tolerant of extreme conditions.

公开(公告)号：EP3129475A2

公开(公告)日：2017-02-15

申请号：EP15759935.8

申请日：2015-04-10

Applicant: King Abdullah University of Science and Technology

Inventor： HAMDAN, Samir M. ,  TAKAHASHI, Masateru

IPC: C12N9/12 ,  C12Q1/68

CPC classification number: C12N9/1252 ,  C12P19/34 ,  C12Y207/07007

Abstract: A DNA polymerase composition for amplifying nucleic acids can be tolerant of extreme conditions.

Abstract translation: 用于扩增核酸的DNA聚合酶组合物可以耐受极端条件。