公开(公告)号：EP2353002A1

公开(公告)日：2011-08-10

申请号：EP08878142.2

申请日：2008-11-11

Applicant: KIST Korea Institute of Science and Technology

Inventor： HWANG, Kyo Seon ,  KIM, Tae Song ,  KIM, Sang Kyung ,  LEE, Sang Myung ,  LEE, Yoon Sik

IPC: G01N33/53

CPC classification number: G01N33/54373

Abstract: Provided is a protein detection system using a micro-cantilever and based on immune responses, wherein the micro-cantilever shows significantly improved sensitivity to allow detection of a trace amount of biomolecule. To the micro-cantilever, sandwich immunoassay is applied, and the sandwich immunoassay uses a polyclonal antibody or silica nanoparticles having a monoclonal antibody bound thereto, so that variations in the output signals of the cantilever are amplified and the detection sensitivity is significantly improved. The system enables detection of disease specific antigen at several femtomolar levels, and makes it possible to detect a trace amount of protein related to diseases, particularly to cancers, with ease.