公开(公告)号：US20170261513A1

公开(公告)日：2017-09-14

申请号：US15504732

申请日：2015-08-19

Applicant: KOREA RESEARCH INSTITUTE OF STANDARDS AND SCIENCE

Inventor： Dukjin KANG ,  Sun Young LEE ,  Sook-Kyung KIM

IPC: G01N33/68 ,  H01J49/00

CPC classification number: G01N33/6848 ,  C12N9/50 ,  G01N33/6842 ,  G01N2440/14 ,  H01J49/0027 ,  H01J49/004

Abstract: A phosphoprotein extraction method and a mass spectrometric method using a microbore hollow fiber enzymatic reactor (mHFER) based antigen-antibody reaction and, specifically, to an extraction method and a mass spectrometric method, wherein phosphoproteins or phosphopeptides present in the body are extracted using phosphoserine-, phosphothreonine-, and phosphotyrosine-antibodies, and measured by a mass spectrometer, and thus biomarker phosphoproteins for diagnosis of diseases are found, contributing to early diagnosis of diseases. The mass spectrometric method using the antigen-antibody reaction based extraction method can: minimize temporal and economic burdens resulting from a low extraction rate and a complicated sample pre-treatment; increase the extraction efficiency by using a considerable number of phosphopeptides (or phosphoproteins) and antibodies with strong affinity; and allow the extraction of low-concentration phosphopeptides or phosphoproteins, and thus is expected to have high applicability in discovering disease diagnosis protein markers and identifying and studying mechanisms thereof.