公开(公告)号：JPS5783295A

公开(公告)日：1982-05-25

申请号：JP15727980

申请日：1980-11-08

Applicant: Kiyoshi Tachibana

Inventor： TACHIBANA KIYOSHI ,  OKA MASANORI

IPC: C12Q1/26 ,  G01N33/82

Abstract: PURPOSE: To determine vitamin B
2 in a test sample quickly with saving energy, by reacting the test sample with an enzyme to oxidize vitamin B
2 at its 5'- position and an electron acceptor, followed by measuring the formed reduction type electron acceptor.
CONSTITUTION: A test sample is reacted with an enzyme obtained form Schizophyllum commune to oxideze vitamin B
2 at its 5'-position, and an electron acceptor, e.g., 2,6-dichlorophenolindolephenol, Methylene Blue, phenazine methosulfate, etc. After they are reacted in a pH of about 4W8.5 at about 20W50°C for about 5minW48hr, the prepared reduction type electron acceptor is measured directly or after it is introduced to other coloring system, to determine an amount of B
2 in the test sample.
COPYRIGHT: (C)1982,JPO&Japio

Abstract translation: 目的：快速测定维生素B2在节省能量的同时，通过使测试样品与酶在5'位置氧化维生素B2和电子受体，然后测量形成的还原型电子受体。 构成：试验样品与裂殖酵母菌（Schizophyllum commune）获得的酶反应，在其5'位上氧化维生素B2，电子受体例如2,6-二氯苯酚吲哚酚，亚甲基蓝，吩嗪甲硫酸盐等。反应后 在约20-50℃的pH约4-8.5约5分-48小时，所制备的还原型电子受体直接测量或引入其它着色系统后，测定试验中的B2量 样品。