公开(公告)号：EP2187213B1

公开(公告)日：2013-04-24

申请号：EP09168598.2

申请日：2009-08-25

Applicant: Korea Basic Science Institute

Inventor： Lee, Zee Won ,  Kim, Soo Hyun

IPC: G01N33/542 ,  G01N33/557 ,  G01N33/58 ,  G01N33/68 ,  G01N33/50 ,  C12N15/10

CPC classification number: G01N33/5035 ,  C07K2319/01 ,  C07K2319/60 ,  G01N33/542 ,  G01N33/557 ,  G01N33/582 ,  G01N33/6845

公开(公告)号：EP2187213A1

公开(公告)日：2010-05-19

申请号：EP09168598.2

申请日：2009-08-25

Applicant: Korea Basic Science Institute

Inventor： Lee, Zee Won ,  Kim, Soo Hyun

IPC: G01N33/542 ,  G01N33/557 ,  G01N33/58 ,  G01N33/68 ,  G01N33/50 ,  C12N15/10

CPC classification number: G01N33/5035 ,  C07K2319/01 ,  C07K2319/60 ,  G01N33/542 ,  G01N33/557 ,  G01N33/582 ,  G01N33/6845

Abstract: Disclosed is a novel method for detecting interactions of biomolecules. More particularly, the disclosed method includes (a) preparing a cell comprising (i) a first construct comprising a bait, a first labeling material and a translocation module; and (ii) a second construct comprising a prey and a second labeling material; (b) detecting the distribution of the first construct and the second construct in the cell. The present invention provides a method capable of detecting bindings and interactions occurring in a living cell in real time, and a method for screening a material that alters the binding and the interaction. The method of the present invention overcomes the disadvantages including inaccuracy and complexity of existing biomaterial interaction detection techniques. By labeling both constructs to promote accuracy, the present invention provides a novel real-time, antibody-free analysis.

Abstract translation: 公开了一种用于检测生物分子的相互作用的新方法。 更具体地，盘方法游离缺失包括：（a）制备细胞，其包含（i）第一构建体，包含诱饵的第一标记物质和易位模块; 和（ii）第二构建体，包含捕食和第二标记物质; （b）检测所述第一构建体在细胞中的分布和所述第二构建体。 本发明提供了能够检测绑定和相互作用进行实时的活细胞内发生的方法，和用于筛选的材料做了年龄的结合和相互作用的方法。 在本发明的方法而来的缺点，包括不准确的和现有的生物材料相互作用的检测技术的复杂性。 通过标记两个构建体，以促进准确度，本发明提供了一种新颖的实时，无抗体的分析。