公开(公告)号：US20100068735A1

公开(公告)日：2010-03-18

申请号：US12556912

申请日：2009-09-10

Applicant: Kui-hyun KIM ,  Beom-seok LEE

Inventor： Kui-hyun KIM ,  Beom-seok LEE

IPC: G01N33/566 ,  C12M1/34

CPC classification number: G01N33/54366 ,  B01L3/5027 ,  B01L2200/10 ,  B01L2300/0645 ,  B01L2300/0806 ,  B01L2300/0867 ,  B01L2400/0409 ,  B01L2400/0487 ,  B01L2400/0677

Abstract: Provided are a microfluidic device including a unit for evaluating a capture material and a method of evaluating a capture material.

Abstract translation: 提供了包括用于评估捕获材料的单元的微流体装置和评估捕获材料的方法。

公开(公告)号：US20070275435A1

公开(公告)日：2007-11-29

申请号：US11625847

申请日：2007-01-23

Applicant: Kui-hyun KIM ,  Jung-im HAN ,  Jun-hong MIN ,  Kwang-ho CHEONG ,  Byung-chul KIM

Inventor： Kui-hyun KIM ,  Jung-im HAN ,  Jun-hong MIN ,  Kwang-ho CHEONG ,  Byung-chul KIM

IPC: C12Q1/02 ,  C12M1/00

CPC classification number: C12M41/36 ,  C12M41/12 ,  C12M41/26 ,  C12M41/34

Abstract: Disclosed herein are a cell culture chip for monitoring a cell culture in real time and a method of monitoring the cell culture using the cell culture chip. The cell culture chip includes a cell culture chamber formed by side walls of a non-conductive material and a bottom layer of an insulating material and capable of accommodating a cell culture media. The cell culture chip also includes a semiconductor layer disposed under the bottom layer, a metal layer disposed under the semiconductor layer, and an electrode disposed in the cell culture chamber. The cell culture chip monitors both the states of cells attached to walls of the cell culture chamber and the states of cells floating in the cell culture media.

Abstract translation: 本文公开了用于实时监测细胞培养物的细胞培养芯片和使用细胞培养芯片监测细胞培养的方法。 细胞培养芯片包括由非导电材料的侧壁和绝缘材料的底层形成并能够容纳细胞培养基的细胞培养室。 细胞培养芯片还包括设置在底层下方的半导体层，设置在半导体层下方的金属层和设置在细胞培养室中的电极。 细胞培养芯片监测附着于细胞培养室壁的细胞状态和悬浮在细胞培养基中的细胞状态。

公开(公告)号：US20070264723A1

公开(公告)日：2007-11-15

申请号：US11684393

申请日：2007-03-09

Applicant: Kui-hyun KIM ,  Byung-chul KIM ,  Su-hyeon KIM

Inventor： Kui-hyun KIM ,  Byung-chul KIM ,  Su-hyeon KIM

IPC: G01N1/00

CPC classification number: C40B60/14 ,  B01J19/0046 ,  B01J2219/00317 ,  B01J2219/00369 ,  B01J2219/00371 ,  B01J2219/00385 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00574 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00655 ,  B01J2219/00657 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00731 ,  B01J2219/00743 ,  B01L3/0268 ,  B01L2400/027 ,  B01L2400/043 ,  G01N2035/1034 ,  Y10T436/25

Abstract: An apparatus for printing a biomolecular droplet onto a substrate using an electric charge concentration effect includes; an electric field forming electrode including an accommodating area in which the biomolecular droplet including micro magnetic beads is accommodated and a nozzle formed on an end of the accommodating area through which the biomolecular droplet is discharged, a substrate disposed below the electric field forming electrode, including a grounded target surface onto which the biomolecular droplet discharged from the nozzle of the electric field forming electrode is deposited, a magnet disposed below the substrate which applies a magnetic force on the micro magnetic beads, and an open circuit type voltage applying unit electrically connected to the electric field forming electrode which applies a charge to the electric field forming electrode which generates an electrical force which causes the biomolecular droplet to be ejected onto the target surface of the substrate.

Abstract translation: 使用电荷浓度效应将生物分子液滴印刷到基板上的装置包括： 包括容纳区域的电场形成电极，其中容纳包括微磁珠的生物分子液滴和形成在所述容纳区域的生物分子液滴被排出的端部的喷嘴，设置在所述电场形成电极下方的衬底，包括 沉积从电场形成电极的喷嘴排出的生物分子滴的接地目标表面，设置在基板上的对微小磁珠施加磁力的磁体，以及开路型电压施加单元，电连接到 该电场形成电极向电场形成电极施加电荷，该电场产生使生物分子液滴喷射到基板的目标表面上的电力。

公开(公告)号：US20100329931A1

公开(公告)日：2010-12-30

申请号：US12833095

申请日：2010-07-09

Applicant: Kyu-tae YOO ,  Joon-ho KIM ,  Jun-hong MIN ,  Sung-ouk JUNG ,  Ji-na NAMGOONG ,  Kui-hyun KIM ,  Jeo-young SHIM ,  Kak NAMKOONG

Inventor： Kyu-tae YOO ,  Joon-ho KIM ,  Jun-hong MIN ,  Sung-ouk JUNG ,  Ji-na NAMGOONG ,  Kui-hyun KIM ,  Jeo-young SHIM ,  Kak NAMKOONG

IPC: G01N27/00

CPC classification number: G01N33/54373

Abstract: Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.

Abstract translation: 提供了一种感测开关和使用该感测开关的感测方法。 感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。 目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。

公开(公告)号：US20090170211A1

公开(公告)日：2009-07-02

申请号：US12272183

申请日：2008-11-17

Applicant: Kyu-tae YOO ,  Joon-ho KIM ,  Jun-hong MIN ,  Sung-ouk JUNG ,  Ji-na NAMGOONG ,  Kui-hyun KIM ,  Jeo-young SHIM ,  Kak NAMKOONG

Inventor： Kyu-tae YOO ,  Joon-ho KIM ,  Jun-hong MIN ,  Sung-ouk JUNG ,  Ji-na NAMGOONG ,  Kui-hyun KIM ,  Jeo-young SHIM ,  Kak NAMKOONG

IPC: G01N27/00

CPC classification number: G01N33/54373

Abstract: Provided are a sensing switch and a sensing method using the same. The sensing switch includes: a substrate; a supporter on the substrate; a sensing plate that is connected to a side of the supporter and is in parallel with the substrate by a predetermined distance; a receptor binding region on an upper surface of an end portion of the sensing plate; an electric or magnetic field generation device that induces deflection of the sensing plate when a receptor bound to the receptor binding region is selectively bound to an electrically or magnetically active ligand; and a pair of switching electrodes that are separated by a predetermined distance and is connected when the sensing plate contacts the substrate due to the deflection of the sensing plate. A target material need not be labelled, a signal processing of a fluorescent or electrical detection signal using an analysis apparatus is not required, and a signal can be directly decoded by confirming whether a current flows through the switch.

Abstract translation: 提供了一种感测开关和使用该感测开关的感测方法。 感测开关包括：基板; 底物上的支撑物; 感测板，其与所述支撑体的一侧连接并且与所述基板平行预定距离; 在感测板的端部的上表面上的受体结合区域; 当与受体结合区结合的受体选择性地结合到电或磁性活性配体时，引起感测板偏转的电场或磁场产生装置; 以及一对分离预定距离的开关电极，并且当感测板由于感测板的偏转而与基板接触时被连接。 目标材料不需要标注，不需要使用分析装置的荧光或电检测信号的信号处理，并且可以通过确认电流是否流过开关直接解码信号。

公开(公告)号：US20070263037A1

公开(公告)日：2007-11-15

申请号：US11747043

申请日：2007-05-10

Applicant: Kui-hyun KIM ,  Byung-chul KIM ,  Kwang-ho CHEONG ,  Su-hyeon KIM

Inventor： Kui-hyun KIM ,  Byung-chul KIM ,  Kwang-ho CHEONG ,  Su-hyeon KIM

IPC: B41J2/06

CPC classification number: B01J19/0046 ,  B01J2219/00371 ,  B01J2219/00378 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00641 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00743 ,  B01L3/0262 ,  B01L2300/0819 ,  B01L2400/027 ,  C40B60/14

Abstract: An apparatus and method which print a biomolecular droplet onto a solid substrate using an electric charge concentration effect comprises: a needle shaped electric field forming electrode which is made of a conductive material, is disposed vertically, and comprises an accommodating area and a nozzle formed on a bottom end of the accommodating area; a solid substrate which is electrically grounded, is disposed below the electric field forming electrode, and comprises a moisture thin film and a target surface onto which the biomolecular droplet is discharged from the nozzle of the electric field forming electrode; and an open circuit type voltage applying unit which is electrically connected to the electric field forming electrode, applies a charge to the electric field forming electrode, and causes the biomolecular droplet to be ejected onto the target surface.

Abstract translation: 使用电荷浓度效应将生物分子液滴印刷到固体基质上的装置和方法包括：由导电材料制成的针形电场形成电极垂直设置，并且包括容纳区域和形成在 容纳区域的底端; 电气接地的固体基板设置在电场形成电极的下方，并且包括湿气薄膜和生物分子液滴从电场形成电极的喷嘴排出的目标表面; 以及与电场形成电极电连接的开路型电压施加单元，向电场形成电极施加电荷，并使生物分子液滴喷射到目标表面上。