公开(公告)号：US09464316B2

公开(公告)日：2016-10-11

申请号：US14035890

申请日：2013-09-24

Applicant: LIFE TECHNOLOGIES AS

Inventor： Erling Sigurd Finne

IPC: C07H21/00 ,  C12Q1/68 ,  C12N15/10

CPC classification number: C12Q1/6806 ,  C12N15/1013

Abstract: A method of isolating nucleic acid from a sample is provided that includes contacting a sample that includes nucleic acid with a solid support, wherein the surface of the solid support includes groups that can complex with the nucleic acid, in the presence of a solution that includes an oligoethylene glycol having 10 or fewer ethylene oxide-units and a salt comprising a monovalent or divalent metal ion, whereby soluble nucleic acid in said sample binds to the surface of the support; separating the solid support with bound nucleic acid from the sample; and eluting the bound nucleic acid from the solid support, thereby isolating nucleic acid from the sample. Also provided is a method of washing nucleic acids bound to a solid support that includes providing a solid support, wherein nucleic acid molecules are bound to the surface of the solid support; and washing the solid support with a solution that includes an oligoethylene glycol having 10 or fewer ethylene oxide units.

Abstract translation: 提供了从样品中分离核酸的方法，其包括将包含核酸的样品与固体支持物接触，其中所述固体支持物的表面包括可与核酸复合的基团，所述溶液包括 具有10个或更少环氧乙烷单元的低聚乙二醇和包含一价或二价金属离子的盐，由此所述样品中的可溶性核酸与载体表面结合; 从样品中分离具有结合核酸的固体支持物; 并从固体支持物洗脱结合的核酸，从而从样品中分离核酸。 还提供了洗涤与固体支持物结合的核酸的方法，其包括提供固体支持物，其中核酸分子与固体支持物的表面结合; 并用包含具有10个或更少环氧乙烷单元的低聚乙二醇的溶液洗涤固体载体。