公开(公告)号：EP2489729A3

公开(公告)日：2012-09-26

申请号：EP12159304.0

申请日：2007-09-28

Applicant: LOMA LINDA UNIVERSITY

Inventor： Li, Fengchun ,  Escher, Alan P.

IPC: C12N5/10 ,  A61P37/06 ,  A61K31/713 ,  C12N15/113

CPC classification number: C12N5/0602 ,  A61K31/7088 ,  A61K35/12 ,  A61K39/001 ,  A61K2035/122 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/531

Abstract: Mammalian host cells for use in a cell-mediated transfection process, which contain an RNAi molecule and an expression vector for a pro-apoptotic protein. The method includes inducing apoptotic cell (AC) death in mammalian cells that contain an RNAi molecule capable of down-regulating a chosen target gene. Living cells expressing the target gene are then exposed to the ACs. The ACs are processed by the living cells, and the RNAi molecule in the ACs down-regulates the expression of the target gene in living cells.

公开(公告)号：EP2489729A2

公开(公告)日：2012-08-22

申请号：EP12159304.0

申请日：2007-09-28

Applicant: LOMA LINDA UNIVERSITY

Inventor： Li, Fengchun ,  Escher, Alan P.

IPC: C12N5/10 ,  A61P37/06 ,  A61K31/713 ,  C12N15/113

CPC classification number: C12N5/0602 ,  A61K31/7088 ,  A61K35/12 ,  A61K39/001 ,  A61K2035/122 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/531

Abstract: Mammalian host cells for use in a cell-mediated transfection process, which contain an RNAi molecule and an expression vector for a pro-apoptotic protein. The method includes inducing apoptotic cell (AC) death in mammalian cells that contain an RNAi molecule capable of down-regulating a chosen target gene. Living cells expressing the target gene are then exposed to the ACs. The ACs are processed by the living cells, and the RNAi molecule in the ACs down-regulates the expression of the target gene in living cells.

公开(公告)号：EP2868743B1

公开(公告)日：2017-11-15

申请号：EP15152934.4

申请日：2007-09-28

Applicant: LOMA LINDA UNIVERSITY

Inventor： Li, Fengchun ,  Escher, Alan P.

IPC: C12N5/071 ,  C12N15/113 ,  A61K31/7088

CPC classification number: C12N5/0602 ,  A61K31/7088 ,  A61K35/12 ,  A61K39/001 ,  A61K2035/122 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/531

Abstract: Mammalian host cells for use in a cell-mediated transfection process, which contain an RNAi molecule and an expression vector for a pro-apoptotic protein. The method includes inducing apoptotic cell (AC) death in mammalian cells that contain an RNAi molecule capable of down-regulating a chosen target gene. Living cells expressing the target gene are then exposed to the ACs. The ACs are processed by the living cells, and the RNAi molecule in the ACs down-regulates the expression of the target gene in living cells.

公开(公告)号：EP2058393A1

公开(公告)日：2009-05-13

申请号：EP09000207.2

申请日：2006-05-05

Applicant: LOMA LINDA UNIVERSITY

Inventor： Escher, Alan P. ,  Li, Fengchun

IPC: C12N15/00 ,  A61K31/713 ,  C07H21/04

CPC classification number: A61K39/0008 ,  A61K2039/53 ,  A61K2039/55516

Abstract: A composition for preventing, delaying the onset of or treating one or more than one immune-mediated inflammatory disorder in an organism who is susceptible to developing the immune-mediated inflammatory disorder, who is developing the immune-mediated inflammatory disorder or who has the immune-mediated inflammatory disorder. A method of preventing, delaying the onset of or treating an organism who is susceptible to developing the immune-mediated inflammatory disorder, who is developing the immune-mediated inflammatory disorder or who has the immune-mediated inflammatory disorder.

Abstract translation: 一种用于预防，延迟发作或治疗一种或在生物体谁是易于发展的免疫介导的炎性病症，谁正在开发免疫介导的炎性病症或谁多于一个的免疫介导的炎性疾病的组合物具有免疫 介导的炎症性疾病。 预防，延迟发作或在生物体谁是易于发展的免疫介导的炎性病症，谁正在开发免疫介导的炎性病症或谁具有免疫介导的炎性病症治疗的方法。

公开(公告)号：EP1626083A1

公开(公告)日：2006-02-15

申请号：EP05021002.0

申请日：2003-08-06

Applicant: LOMA LINDA UNIVERSITY

Inventor： Escher, Alan P. ,  Li, Fengchun

IPC: C12N5/00 ,  C12N15/00 ,  C12N15/87 ,  A61K48/00

CPC classification number: A61K48/00 ,  A61K38/1709 ,  A61K39/0008 ,  A61K48/005 ,  A61K2039/53 ,  A61K2039/55516 ,  C07K14/005 ,  C07K14/4713 ,  C07K14/4747 ,  C12N5/00 ,  C12N15/00 ,  C12N15/87 ,  C12N2710/10322 ,  C12N2840/203

Abstract: A substance is disclosed for preventing, delaying the onset of, or treating one or more than one autoimmune disease, the substance comprising a polynucleotide construct comprising a polynucleotide sequence encoding the adenoviral protein E3-GP19k and encoding one or more than one antigen for the autoimmune disease. A use is also disclosed of a polynucleotide construct comprising a combination of a polynucleotide construct comprising a polynucleotide sequence encoding the adenoviral protein E3-GP19k and encoding one or more than one autoantigen for the autoimmune disease for the manufacture of a medicament for preventing, delaying the onset of or treating one or more than one autoimmune disease.

Abstract translation: 公开了用于预防，延迟或治疗一种或多种自身免疫性疾病的物质，所述物质包含多核苷酸构建体，所述多核苷酸构建体包含编码腺病毒蛋白质E3-GP19k并编码一种或多种抗原用于自身免疫的多核苷酸序列 疾病。 还公开了包含含有编码腺病毒蛋白质E3-GP19k的多核苷酸序列并编码一种或多种自身免疫性疾病自身抗原的多核苷酸构建体的多核苷酸构建体在制备用于预防，延缓 发作或治疗一种或多种自身免疫性疾病。

公开(公告)号：EP2489729B1

公开(公告)日：2015-02-25

申请号：EP12159304.0

申请日：2007-09-28

Applicant: LOMA LINDA UNIVERSITY

Inventor： Li, Fengchun ,  Escher, Alan P.

IPC: A61K31/713 ,  C12N15/113 ,  A61P37/06 ,  A61K35/12

CPC classification number: C12N5/0602 ,  A61K31/7088 ,  A61K35/12 ,  A61K39/001 ,  A61K2035/122 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/531

Abstract: Mammalian host cells for use in a cell-mediated transfection process, which contain an RNAi molecule and an expression vector for a pro-apoptotic protein. The method includes inducing apoptotic cell (AC) death in mammalian cells that contain an RNAi molecule capable of down-regulating a chosen target gene. Living cells expressing the target gene are then exposed to the ACs. The ACs are processed by the living cells, and the RNAi molecule in the ACs down-regulates the expression of the target gene in living cells.

公开(公告)号：EP2058393B1

公开(公告)日：2010-07-07

申请号：EP09000207.2

申请日：2006-05-05

Applicant: LOMA LINDA UNIVERSITY

Inventor： Escher, Alan P. ,  Li, Fengchun

IPC: C12N15/00 ,  A61K31/713 ,  C07H21/04

CPC classification number: A61K39/0008 ,  A61K2039/53 ,  A61K2039/55516

公开(公告)号：EP1626083B1

公开(公告)日：2006-11-02

申请号：EP05021002.0

申请日：2003-08-06

Applicant: LOMA LINDA UNIVERSITY

Inventor： Escher, Alan P. ,  Li, Fengchun

IPC: C12N5/00 ,  C12N15/00 ,  C12N15/87 ,  A61K48/00

CPC classification number: A61K48/00 ,  A61K38/1709 ,  A61K39/0008 ,  A61K48/005 ,  A61K2039/53 ,  A61K2039/55516 ,  C07K14/005 ,  C07K14/4713 ,  C07K14/4747 ,  C12N5/00 ,  C12N15/00 ,  C12N15/87 ,  C12N2710/10322 ,  C12N2840/203

Abstract: A substance is disclosed for preventing, delaying the onset of or treating one or more than one autoimmune disease, the substance comprising a combination of a polynucleotide construct comprising a polynucleotide sequence encoding ”BCL-2 and encoding one or more than one autoantigen for the autoimmune disease. A use is also disclosed of a polynucleotide construct comprising a combination of a polynucleotide construct comprising a polynucleotide sequence encoding ”BCL-2 and encoding one or more than one autoantigen for the autoimmune disease, for the manufacture of a medicament for preventing, delaying the onset of or treating one or more than one autoimmune disease.

公开(公告)号：EP1624053B1

公开(公告)日：2006-11-02

申请号：EP05021001.2

申请日：2003-08-06

Applicant: LOMA LINDA UNIVERSITY

Inventor： Escher, Alan P. ,  Li, Fengchun

IPC: C12N5/00 ,  C12N15/00 ,  C12N15/87 ,  A61K48/00

CPC classification number: A61K48/00 ,  A61K38/1709 ,  A61K39/0008 ,  A61K48/005 ,  A61K2039/53 ,  A61K2039/55516 ,  C07K14/005 ,  C07K14/4713 ,  C07K14/4747 ,  C12N5/00 ,  C12N15/00 ,  C12N15/87 ,  C12N2710/10322 ,  C12N2840/203

Abstract: A substance is disclosed for preventing, delaying the onset of or treating one or more than one autoimmune disease, the substance comprising a combination of a polynucleotide construct comprising a polynucleotide sequence encoding ”BCL-2 and encoding one or more than one autoantigen for the autoimmune disease. A use is also disclosed of a polynucleotide construct comprising a combination of a polynucleotide construct comprising a polynucleotide sequence encoding ”BCL-2 and encoding one or more than one autoantigen for the autoimmune disease, for the manufacture of a medicament for preventing, delaying the onset of or treating one or more than one autoimmune disease.

公开(公告)号：EP2868743A3

公开(公告)日：2015-05-27

申请号：EP15152934.4

申请日：2007-09-28

Applicant: LOMA LINDA UNIVERSITY

Inventor： Li, Fengchun ,  Escher, Alan P.

IPC: C12N5/071 ,  C12N15/113 ,  A61K31/7088

CPC classification number: C12N5/0602 ,  A61K31/7088 ,  A61K35/12 ,  A61K39/001 ,  A61K2035/122 ,  C12N15/113 ,  C12N2310/14 ,  C12N2310/531

Abstract: Mammalian host cells for use in a cell-mediated transfection process, which contain an RNAi molecule and an expression vector for a pro-apoptotic protein. The method includes inducing apoptotic cell (AC) death in mammalian cells that contain an RNAi molecule capable of down-regulating a chosen target gene. Living cells expressing the target gene are then exposed to the ACs. The ACs are processed by the living cells, and the RNAi molecule in the ACs down-regulates the expression of the target gene in living cells.

Abstract translation: 用于细胞介导的转染过程的哺乳动物宿主细胞，其含有RNAi分子和促凋亡蛋白的表达载体。 该方法包括在含有能够下调所选靶基因的RNAi分子的哺乳动物细胞中诱导凋亡细胞（AC）死亡。 然后将表达靶基因的活细胞暴露于AC。 AC由活细胞处理，并且AC中的RNAi分子下调活细胞中靶基因的表达。