公开(公告)号：EP0934425B1

公开(公告)日：2003-11-19

申请号：EP97943558.3

申请日：1997-09-24

Applicant: LOMA LINDA UNIVERSITY

Inventor： SZALAY, Aldar, A. ,  WANG, Gefu ,  WANG, Yubao

IPC: C12P21/04 ,  C12P21/06 ,  C12N1/20 ,  C12N9/02 ,  C12N15/09 ,  C07K14/00 ,  C07K16/00 ,  C07H21/04

CPC classification number: C12N9/001 ,  C07K14/43595 ,  C07K16/40 ,  C07K2319/00

Abstract: A fusion gene is provided comprising the cDNA of Renilla luciferase and the cDNA of the 'humanized' Aequorea green fluorescent protein. The fusion gene was used to produce a novel protein, the 'Renilla-GFP fusion protein', which displayed both the luciferase activity of Renilla luciferase, and the green fluorescence of GFP. The Renilla-GFP fusion gene is useful as a double marker for monitoring gene expression quantitatively in UV light and by enzyme activity.

Abstract translation: 提供融合基因，其包含海肾萤光素酶的cDNA和“人源化”水母绿色荧光蛋白的cDNA。 融合基因用于产生新的蛋白质，“Renilla-GFP融合蛋白”，其显示了海肾萤光素酶的荧光素酶活性和GFP的绿色荧光。 Renilla-GFP融合基因可用作紫外光和酶活性定量监测基因表达的双重标记。

公开(公告)号：EP0934425A1

公开(公告)日：1999-08-11

申请号：EP97943558.0

申请日：1997-09-24

Applicant: LOMA LINDA UNIVERSITY

Inventor： SZALAY, Aldar, A. ,  WANG, Gefu ,  WANG, Yubao

IPC: C12N15 ,  C07K14 ,  C07K16 ,  C07K19 ,  C12N9 ,  C12P21

CPC classification number: C12N9/001 ,  C07K14/43595 ,  C07K16/40 ,  C07K2319/00

Abstract: A fusion gene is provided comprising the cDNA of Renilla luciferase and the cDNA of the 'humanized' Aequorea green fluorescent protein. The fusion gene was used to produce a novel protein, the 'Renilla-GFP fusion protein', which displayed both the luciferase activity of Renilla luciferase, and the green fluorescence of GFP. The Renilla-GFP fusion gene is useful as a double marker for monitoring gene expression quantitatively in UV light and by enzyme activity.

公开(公告)号：WO1998014605A1

公开(公告)日：1998-04-09

申请号：PCT/US1997017162

申请日：1997-09-24

Applicant: LOMA LINDA UNIVERSITY

Inventor： LOMA LINDA UNIVERSITY ,  SZALAY, Aldar, A. ,  WANG, Gefu ,  WANG, Yubao

IPC: C12P21/04

CPC classification number: C12N9/001 ,  C07K14/43595 ,  C07K16/40 ,  C07K2319/00

Abstract: A fusion gene is provided comprising the cDNA of Renilla luciferase and the cDNA of the "humanized" Aequorea green fluorescent protein. The fusion gene was used to produce a novel protein, the "Renilla-GFP fusion protein", which displayed both the luciferase activity of Renilla luciferase, and the green fluorescence of GFP. The Renilla-GFP fusion gene is useful as a double marker for monitoring gene expression quantitatively in UV light and by enzyme activity.

Abstract translation: 提供融合基因，其包含海肾萤光素酶的cDNA和“人源化”水稻绿色荧光蛋白的cDNA。 融合基因用于产生新的蛋白质，“Renilla-GFP融合蛋白”，其显示了海肾萤光素酶的荧光素酶活性和GFP的绿色荧光。 Renilla-GFP融合基因可用作紫外光和酶活性定量监测基因表达的双重标记。