公开(公告)号：US5780255A

公开(公告)日：1998-07-14

申请号：US488510

申请日：1995-06-09

Applicant: Luigi Preda

Inventor： Luigi Preda

IPC: G01N33/50 ,  C12Q1/56 ,  G01N33/86 ,  C12Q1/37 ,  C12N9/48 ,  C12N9/74

CPC classification number: G01N33/86 ,  G01N2333/4626 ,  G01N2333/745 ,  G01N2333/96461 ,  G01N2333/96463 ,  G01N2405/04

Abstract: The present invention provides a method for determining thrombotic risk in an individual. The method involves determining the activity of Protein C and Protein S in the plasma of the individual thought to be at thrombotic risk by adding to a plasma sample obtained from the individual (i) a first reagent in an amount sufficient to induce or activate coagulation in the plasma, (ii) a second reagent which activates endogenous protein C in the plasma, and (iii) a third reagent comprising calcium salts, phospholipids or tissue thromboplastin, or a combination thereof. To a second plasma sample from the same subject is added a reagent which induces or activates coagulation, and a buffer or other material which does not activate protein C, and a third reagent as described above. The time, rate or both, necessary for the conversion of endogenous fibrinogen to fibrin in both the first and second samples is measured. The same steps are performed on normal control plasma, and the difference or ratio in the times, rates, or both, obtained above are determined. The difference or ratio is indicative of the thrombotic risk in the subject. A kit adapted to carry out the method also is the subject of the present invention. The methods and kits of the invention in other embodiments may comprise a first reagent comprising a synthetic substrate, a second reagent which in the first sample from the subject activates protein C, and in the second sample, a second reagent which does not activate protein C. In these embodiments, the rates of hydrolysis of the synthetic substrates are measured and compared.

Abstract translation: 本发明提供了确定个体血栓形成风险的方法。 该方法包括通过加入从个体获得的血浆样品（i）以足以诱导或激活凝血的量的第一试剂来确定被认为是血栓形成风险的个体血浆中的蛋白C和蛋白S的活性 血浆，（ii）激活血浆中的内源蛋白C的第二试剂，和（iii）包含钙盐，磷脂或组织凝血激酶或其组合的第三试剂。 向来自相同受试者的第二血浆样品中加入诱导或活化凝血剂的试剂，以及不活化蛋白质C的缓冲液或其它物质，以及如上所述的第三试剂。 测量在第一和第二样品中将内源纤维蛋白原转化为纤维蛋白所需的时间，速率或两者。 对正常对照等离子体进行相同的步骤，并确定上述得到的时间，速率或二者的差异或比例。 差异或比例表明受试者的血栓形成风险。 适于实施该方法的套件也是本发明的主题。 在其它实施方案中，本发明的方法和试剂盒可以包括第一试剂，其包含合成底物，来自受试者的第一样品中的第二试剂激活蛋白C，而在第二样品中，第二试剂不会激活蛋白C 在这些实施方案中，测量和比较合成基材的水解速率。

公开(公告)号：US5147805A

公开(公告)日：1992-09-15

申请号：US548224

申请日：1990-06-29

Applicant: Luigi Preda ,  Antonio Lombardi

Inventor： Luigi Preda ,  Antonio Lombardi

IPC: C12Q1/37 ,  C12Q1/56 ,  G01N33/86

CPC classification number: G01N33/86 ,  C12Q1/56 ,  G01N2333/4626 ,  G01N2333/745 ,  G01N2333/96461 ,  Y10S435/975

Abstract: To determine the biological activity of protein S in a sample of human plasma, the suitably diluted sample is added to a substrate formed from plasma deficient in protein S, in which protein C is activated. The functionality of protein is evaluated by a coagulometric test using bovine thromboplastin with added calcium as the phospholipid source. Also disclosed is a kit for determining the activity of Protein S in a human plasma sample. The kit includes a first reagent which is human plasma deficient in protein S, a second reagent which contains an activator for protein C, and a third reagent which contains bovine thromboplastin.

公开(公告)号：US4777141A

公开(公告)日：1988-10-11

申请号：US799675

申请日：1985-11-19

Applicant: Claudio Calzi ,  Luigi Preda

Inventor： Claudio Calzi ,  Luigi Preda

IPC: G01N21/07 ,  G01N21/51 ,  G01N21/82 ,  G01N33/48 ,  G01N33/49 ,  G01N33/86 ,  G01N21/47

CPC classification number: G01N21/82 ,  G01N33/4905 ,  G01N21/07 ,  G01N21/51 ,  Y10S436/909 ,  Y10T436/111666 ,  Y10T436/25375

Abstract: An instrument for measuring coagulation parameters is provided in which plasma and at least one reagent are mixed by spinning in a cuvette with transparent windows and in which measurement is then made of scatter by the mixture of the energy of a light beam sent into the mixture.Upon spin, the plasma components and reagent are displaced into the chamber, thus determining the initiation of the presence of the mixture in which the clot forms, and a photodetecting unit senses the change in the energy of the scattered light, caused by clot formation.

Abstract translation: 提供了一种用于测量凝固参数的仪器，其中等离子体和至少一种试剂通过在具有透明窗口的比色皿中旋转而混合，并且其中通过发射到混合物中的光束的能量的混合物进行测量。 在旋转时，等离子体组分和试剂置换到室中，从而确定凝块形成的混合物的存在的开始，并且光电检测单元感测由凝块形成引起的散射光的能量变化。