公开(公告)号：JP2012072148A

公开(公告)日：2012-04-12

申请号：JP2011231730

申请日：2011-10-21

Applicant: Metabolix Inc ,  メタボリックス,インコーポレイテッド

Inventor： MARTIN DAVID P ,  PEOPLES OLIVER P ,  WILLIAMS SIMON F ,  ZHONG LUHUA

IPC: A61K31/335 ,  A23K1/16 ,  A23K1/18 ,  A23L1/30 ,  A61K31/00 ,  A61K31/19 ,  A61K31/22 ,  A61K31/23 ,  A61K31/765 ,  A61P3/00 ,  A61P3/04 ,  A61P3/10 ,  A61P7/00 ,  A61P9/00 ,  A61P21/04 ,  A61P25/00 ,  A61P25/08 ,  A61P25/28 ,  A61P43/00 ,  C07C69/675 ,  C07D323/00

CPC classification number: A61K31/765 ,  A23K20/105 ,  A23K50/10 ,  A23L33/10 ,  A23V2002/00 ,  A61K31/00 ,  A61K31/19 ,  A23V2250/206 ,  A23V2250/21 ,  A23V2250/30

Abstract: PROBLEM TO BE SOLVED: To provide a nutritional or a therapeutic composition for increasing ketone body levels in the blood of mammals.SOLUTION: There is provided the nutritional or therapeutic composition for increasing the blood ketone levels during oral ingestion including a 3-hydroxy acid (wherein, the 3-hydroxy acid is not 3-hydroxybutyric acid) as an active ingredient. In the composition, the 3-hydroxy acid is in a form selected from the group consisting of: (a) linear oligomers of 3-hydroxy acids in combination with acetoacetate; (b) cyclic oligolides of the 3-hydroxy acids; (c) esters of the 3-hydroxy acids in combination with the acetoacetate; (d) esters of 3-hydroxy acid linear oligomers and cyclic oligolides in combination with the acetoacetate; and (e) combinations thereof. The composition is useful for increasing cardiotonic effects, treatment of diabetes and insulin resistant states, and treatment of effects of neurodegenerative disorders and epilepsy.

Abstract translation: 待解决的问题：提供用于增加哺乳动物血液中酮体水平的营养或治疗组合物。 提供了用于在口服摄取期间增加血液酮水平的营养或治疗组合物，包括3-羟基酸（其中，3-羟基酸不是3-羟基丁酸）作为活性成分。 在组合物中，3-羟基酸为选自下组的形式：（a）3-羟基酸与乙酰乙酸酯的组合的线性低聚物; （b）3-羟基酸的环状寡聚物; （c）3-羟基酸与乙酰乙酸酯的组合的酯; （d）3-羟基酸线性低聚物和环状寡聚物与乙酰乙酸酯的组合的酯; 和（e）它们的组合。 该组合物可用于增加强心作用，治疗糖尿病和胰岛素抵抗状态，以及治疗神经变性疾病和癫痫的作用。 版权所有（C）2012，JPO＆INPIT

公开(公告)号：JP2009261398A

公开(公告)日：2009-11-12

申请号：JP2009144458

申请日：2009-06-17

Applicant: Metabolix Inc ,  Tepha Inc ,  テファ, インコーポレイテッド ,  メタボリックス,インコーポレイテッド

Inventor： MARTIN DAVID P ,  SKRALY FRANK A

IPC: C12P7/62 ,  A61L15/16 ,  A61L27/00 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10

CPC classification number: C12P7/625 ,  Y10T428/31507

Abstract: PROBLEM TO BE SOLVED: To provide genetically engineered organisms for producing PHA (polyhydroxyalkanoate) copolymers containing 2-hydroxyacid monomers, a method for producing the same and a method of application. SOLUTION: The copolymers containing the 2-hydroxyacid monomers are synthesized by a biosynthesis by the action of PHA polymerase in a living cell. By changing the genetic background of the cells, specific metabolic pathways enabling the control of glycolic acid comonomer level in the PHA polymers can be regulated. COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 待解决的问题：提供用于生产含有2-羟基酸单体的PHA（聚羟基链烷酸酯）共聚物的遗传工程生物，其制备方法和应用方法。 解决方案：含有2-羟基酸单体的共聚物通过PHA聚合酶在活细胞中的作用进行生物合成来合成。 通过改变细胞的遗传背景，可以调节能够控制PHA聚合物中乙醇酸共聚单体水平的特异性代谢途径。 版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2007277574A

公开(公告)日：2007-10-25

申请号：JP2007151037

申请日：2007-06-06

Applicant: Metabolix Inc ,  メタボリックス,インコーポレイテッド

Inventor： MARTIN DAVID P ,  PEOPLES OLIVER P ,  WILLIAMS SIMON F

IPC: A01H1/00 ,  C08G63/06 ,  B01D11/02 ,  C08G63/89 ,  C08H99/00 ,  C08L101/16 ,  C12N5/10 ,  C12N15/09 ,  C12P7/62

CPC classification number: C08G63/89 ,  C12P7/625

Abstract: PROBLEM TO BE SOLVED: To provide a method for separating polyhydroxyalkanoate ("PHA") from a plant such as transgenic oil crop plant.
SOLUTION: In order to isolate PHA, in an embodiment, a biomass derived from an oil crop plant is pre-processed, for example, by grinding, crushing or rolling. Then, the oil is extracted from the biomass with the first solvent in which the oil is soluble and the PHA is not highly soluble and the oil is removed. The biomass then can be extracted with the second solvent in which the PHA is soluble, to separate the PHA from the biomass. Alternatively, the PHA-containing biomass is treated with a chemical or biochemical agent, such as an enzyme, to chemically transform the PHA into a PHA derivative. The PHA derivative then is separated from the mixture using, for example, a physical separation process such as distillation, extraction or chromatography.
COPYRIGHT: (C)2008,JPO&INPIT

Abstract translation: 待解决的问题：提供从植物如转基因油料作物植物中分离聚羟基链烷酸酯（“PHA”）的方法。 解决方案：为了分离PHA，在一个实施方案中，来自油料作物植物的生物质经预处理，例如通过研磨，破碎或轧制。 然后，用可溶于油的第一溶剂和PHA不易溶解并除去油的生物质中提取油。 然后可以用可溶于PHA的第二溶剂萃取生物质，以将PHA与生物质分离。 或者，将含PHA的生物质用化学或生化剂如酶处理，以将PHA化学转化为PHA衍生物。 然后使用例如物理分离方法如蒸馏，萃取或色谱法将PHA衍生物与混合物分离。 版权所有（C）2008，JPO＆INPIT

公开(公告)号：JP2003310262A

公开(公告)日：2003-11-05

申请号：JP2003132325

申请日：2003-05-09

Applicant: Metabolix Inc ,  メタボリックス,インコーポレイテッド

Inventor： HUISMAN GJALT W ,  SKRALY FRANK A ,  MARTIN DAVID P ,  PEOPLES OLIVER P

IPC: C12N15/09 ,  C08G63/06 ,  C12N1/21 ,  C12N9/02 ,  C12N9/10 ,  C12N15/53 ,  C12N15/54 ,  C12N15/82 ,  C12P7/52 ,  C12P7/62 ,  C12R1/19

CPC classification number: C12N9/0008 ,  C08G63/06 ,  C12N9/1029 ,  C12N9/1096 ,  C12N15/8243 ,  C12P7/625

Abstract: PROBLEM TO BE SOLVED: To provide a recombinant process to transfer a further gene into a transgenic PHB producer to produce a new strain synthesizing a monomer of a substitute PHA (poly [(R)-3-hydroxyalkanoate]) (e.g. 4HB (copolymer poly (3-hydroxybutyric acid-co-3-hydroxyvaleric acid) (PHBV)) and provide a technique and procedure to stably produce a transgenic organism synthesizing a PHA containing 4-hydroxybutyric acid as the sole constituent or a comonomer. SOLUTION: A gene encoding a heterologous enzyme selected from polyhydroxyalkanoate synthase and 4HB-CoA transferase is stably transferred into a genom to obtain a recombinant host. COPYRIGHT: (C)2004,JPO

Abstract translation: 要解决的问题：提供重组方法将另外的基因转移到转基因PHB生产者中以产生合成替代PHA（聚[（R）-3-羟基链烷酸酯]）单体的新菌株（例如4HB （共聚物（3-羟基丁酸 - 共-3-羟基戊酸）（PHBV）），提供稳定地生产合成含有4-羟基丁酸作为唯一成分或共聚单体的PHA的转基因生物的技术和方法。 >解决方案：将编码选自聚羟基链烷酸合酶和4HB-CoA转移酶的异源酶的基因稳定转移到基因组中以获得重组宿主。（C）2004，JPO

公开(公告)号：JP2009171960A

公开(公告)日：2009-08-06

申请号：JP2008328227

申请日：2008-12-24

Applicant: Metabolix Inc ,  メタボリックス,インコーポレイテッド

Inventor： HUISMAN GJALT W ,  SKRALY FRANK A ,  MARTIN DAVID P ,  PEOPLES OLIVER P

IPC: C12N1/21 ,  C12N15/09 ,  C08G63/06 ,  C12N9/02 ,  C12N9/10 ,  C12N15/53 ,  C12N15/54 ,  C12N15/82 ,  C12P7/52 ,  C12P7/62 ,  C12R1/19

CPC classification number: C12N9/0008 ,  C08G63/06 ,  C12N9/1029 ,  C12N9/1096 ,  C12N15/8243 ,  C12P7/625

Abstract: PROBLEM TO BE SOLVED: To provide a recombinant process capable of incorporating a further gene into a PHB(polyhydroxybutyric acid) producer in order to create a new strain synthesizing a monomer of an alternative polyhydroxyalkanoate(PHA) (e.g. 4-hydroxybutyric acid/4HB), and to provide a method for stably creating an organism synthesizing a 4HB-containing PHA as either of sole construct or comonomer. SOLUTION: The method uses such a recombinant host that its genome is stably incorporated with a gene encoding a heterologous enzyme selected from the group consisting of a PHA synthase and a 4HB-CoA transferase. COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：为了提供能够将另外的基因并入PHB（多羟基丁酸）生产者中的重组方法，以便产生合成替代的聚羟基链烷酸酯（PHA）的单体的新菌株（例如4-羟基丁酸 / 4HB），并且提供用于稳定地产生合成含有4HB的PHA作为唯一构建体或共聚单体的生物体的方法。 解决方案：该方法使用这样的重组宿主，其基因组稳定掺入编码选自PHA合酶和4HB-CoA转移酶的异源酶的基因。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2009112315A

公开(公告)日：2009-05-28

申请号：JP2009045960

申请日：2009-02-27

Applicant: Metabolix Inc ,  メタボリックス,インコーポレイテッド

Inventor： MARTIN DAVID P ,  PEOPLES OLIVER P ,  WILLIAMS SIMON F

IPC: A01H1/00 ,  C12P7/62 ,  C08G63/89 ,  C08H99/00 ,  C12N5/10 ,  C12N15/09

CPC classification number: C08G63/89 ,  C12P7/625

Abstract: PROBLEM TO BE SOLVED: To provide methods for separating polyhydroxyalkanoates ("PHA") from plants, such as transgenic oil crop plants.
SOLUTION: To isolate the PHA, in one embodiment, a biomass derived from an oil crop plant is pre-processed, for example by grinding, crushing or rolling. Then the oil is extracted from the biomass with a first solvent (in which solvent the oil is soluble and the PHA are not highly soluble) to remove the oil. Then the biomass can be extracted with a second solvent (in which solvent the PHA is soluble) to separate the PHA from the biomass. Alternatively, the PHA-containing biomass is treated with a chemical or biochemical agent to chemically transform the PHA into a PHA derivative. Then the PHA derivative is separated from the mixture by using, for example, a physical separation process.
COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 要解决的问题：提供从植物如转基因油料作物植物中分离聚羟基链烷酸酯（“PHA”）的方法。 解决方案：为了分离PHA，在一个实施方案中，来自油料作物植物的生物质被预处理，例如通过研磨，破碎或轧制。 然后用第一溶剂（其​​中油溶于溶剂，PHA不高度溶解）从生物质中提取油，以除去油。 然后生物质可以用第二溶剂（其中PHA溶于其中的溶剂）提取，以将PHA与生物质分离。 或者，用化学或生化试剂处理含PHA的生物质，以将PHA化学转化为PHA衍生物。 然后通过使用例如物理分离方法将PHA衍生物与混合物分离。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2009082147A

公开(公告)日：2009-04-23

申请号：JP2008328226

申请日：2008-12-24

Applicant: Metabolix Inc ,  メタボリックス,インコーポレイテッド

Inventor： HUISMAN GJALT W ,  SKRALY FRANK A ,  MARTIN DAVID P ,  PEOPLES OLIVER P

IPC: C12N1/21 ,  C12N15/09 ,  C08G63/06 ,  C12N9/02 ,  C12N9/10 ,  C12N15/53 ,  C12N15/54 ,  C12N15/82 ,  C12P7/52 ,  C12P7/62 ,  C12R1/19

CPC classification number: C12N9/0008 ,  C08G63/06 ,  C12N9/1029 ,  C12N9/1096 ,  C12N15/8243 ,  C12P7/625

Abstract: PROBLEM TO BE SOLVED: To provide the technology and process for stably making a transgenic organism synthesizing PHA including 4-hydroxy-butyric acid as only one component or as co-monomer by using the recombination process in which another gene is introduced into the transgenic PHP producer, in order to create a new strain synthesizing the substitute PHA monomer (for example, 4-HB). SOLUTION: A recombinant host that stably incorporates the gene encoding different kinds of enzyme selected from polyhydroxyalkanoate synthase and 4HB-CoA transferase into the genome. COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 待解决的问题：提供用于稳定制备转基因生物的技术和方法，其通过使用引入另一种基因的重组方法将包含4-羟基 - 丁酸的PHA作为仅一种成分或作为共聚单体合成 转基因PHP生产者，以产生合成替代PHA单体（例如4-HB）的新菌株。 解决方案：将编码不同种类的选自聚羟基链烷酸合酶和4HB-CoA转移酶的基因的基因稳定并入基因组的重组宿主。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：JP2008248258A

公开(公告)日：2008-10-16

申请号：JP2008180815

申请日：2008-07-10

Applicant: Metabolix Inc ,  メタボリックス,インコーポレイテッド

Inventor： MARTIN DAVID P ,  PEOPLES OLIVER P ,  WILLIAMS SIMON F

IPC: A01H1/00 ,  C08G63/89 ,  C08H99/00 ,  C12N5/10 ,  C12N15/09 ,  C12P7/62

CPC classification number: C08G63/89 ,  C12P7/625

Abstract: PROBLEM TO BE SOLVED: To provide a method for separating polyhydroxyalkanoates ("PHAs") from plants such as a transgenic oil crop plant.
SOLUTION: In an embodiment of the method, to isolate the PHAs, a biomass derived from an oil crop plant is preprocessed by, for example, grinding, crushing or rolling. Oil is then extracted from the biomass by using a first solvent (in which the oil is soluble and the PHAs are not highly soluble) to remove the oil. Then, the biomass can be extracted by using a second solvent (in which the PHAs are soluble) to separate the PHAs from the biomass. Alternatively, the PHA-containing biomass is treated with a chemical or biochemical agent to chemically transform the PHA into a PHA derivative. The PHA derivative is then separated from the mixture by using a physical separation process.
COPYRIGHT: (C)2009,JPO&INPIT

Abstract translation: 待解决的问题：提供从植物如转基因油料作物植物中分离聚羟基链烷酸酯（“PHAs”）的方法。 解决方案：在该方法的一个实施方案中，为了分离PHAs，通过例如研磨，破碎或轧制来预处理来自油料作物的生物质。 然后通过使用第一溶剂（其​​中油可溶而PHAs不是高度可溶的））从生物质中提取油以除去油。 然后，可以通过使用第二溶剂（其中可溶于PHAs）来提取生物质，以将PHAs与生物质分离。 或者，用化学或生化试剂处理含PHA的生物质，以将PHA化学转化为PHA衍生物。 然后通过使用物理分离方法将PHA衍生物与混合物分离。 版权所有（C）2009，JPO＆INPIT

公开(公告)号：WO0119361A3

公开(公告)日：2001-10-04

申请号：PCT/US0025261

申请日：2000-09-14

Applicant: TEPHA INC ,  METABOLIX INC

Inventor： WILLIAMS SIMON F ,  MARTIN DAVID P

IPC: A61K31/19 ,  A61K31/765 ,  A61P5/00 ,  A61P9/10 ,  A61P9/12 ,  A61P15/00 ,  A61P21/02 ,  A61P23/00 ,  A61P25/16 ,  A61P25/18 ,  A61P25/20 ,  A61P25/32 ,  A61P29/00 ,  A61P35/00 ,  A61P43/00 ,  A61P25/00 ,  A61P25/26

CPC classification number: A61K31/19 ,  A61K31/765

Abstract: Oligomers and polymer compositions are provided which comprise GHB and produce GHB after administration in vivo . Devices for the storage and delivery of these polymers and oligomers are also provided. These oligomers and polymer compositions are useful in a variety of applications. The compositions can be used therapeutically, for example, in the treatment of patients with narcolepsy, chronic schizophrenia, catatonic schizophrenia, atypical psychoses, chronic brain syndrome, neurosis, alcoholism, drug addiction and withdrawal, Parkinson's disease and other neuropharmacological illnesses, hypertension, ischemia, circulatory collapse, radiation exposure, cancer, and myocardial infarction. Other uses for the compositions include anesthesia induction, sedation, growth hormone production, heightened sexual desire, anorectic effects, euphoria, smooth muscle relaxation, muscle mass production, and sleep, including rapid eye movement sleep. In a still further embodiment, the oligomers and polymers may be used to produce absence seizures.

Abstract translation: 提供了含有GHB并且在体内给药之后产生GHB的低聚物和聚合物组合物。 还提供了用于储存和递送这些聚合物和低聚物的装置。 这些低聚物和聚合物组合物可用于各种应用中。 组合物可用于治疗例如发作性睡病，慢性精神分裂症，紧张性精神分裂症，非典型精神病，慢性脑综合征，神经症，酒精中毒，药物成瘾和戒断，帕金森病和其他神经药理学疾病，高血压，缺血的患者的治疗 ，循环衰竭，放射线暴露，癌症和心肌梗死。 组合物的其它用途包括麻醉诱导，镇静，生长激素生成，性欲升高，厌食症，欣快，平滑肌松弛，肌肉量产和睡眠，​​包括快速眼动睡眠。 在另一个实施方案中，低聚物和聚合物可以用于产生不发作的发作。

公开(公告)号：WO9851812A3

公开(公告)日：1999-02-11

申请号：PCT/US9809834

申请日：1998-05-12

Applicant: METABOLIX INC

Inventor： WILLIAMS SIMON F ,  MARTIN DAVID P ,  GERNGROSS TILLMAN ,  HOROWITZ DANIEL M

IPC: A61K47/48 ,  A61L15/26 ,  A61L17/10 ,  A61L27/18 ,  A61L27/34 ,  A61L29/06 ,  A61L31/06 ,  C08G63/06 ,  C08G63/685 ,  C08G63/688 ,  C08G63/91 ,  C12N5/00 ,  C12P7/62 ,  C12P7/00

CPC classification number: C12P7/625 ,  A61B17/06166 ,  A61B42/00 ,  A61K47/593 ,  A61K47/6957 ,  A61L15/26 ,  A61L17/10 ,  A61L27/18 ,  A61L27/34 ,  A61L29/06 ,  A61L31/06 ,  C08G63/06 ,  C08G63/6852 ,  C08G63/6882 ,  C08G63/912 ,  C12N5/0068 ,  C12N2533/30 ,  Y10S977/775 ,  Y10T428/139 ,  C08L67/04

Abstract: Polyhydroxyalkanoates (PHAs) from which pyrogen has been removed are provided for use in numerous biomedical applications. PHAs which have been chemically modified to enhance physical and/or chemical properties, for targeting or to modify biodegradability or clearance by the reticuloendothelia system (RES), are described. Methods for depyrogenating PHA polymers prepared by bacterial fermentation processes are also provided, wherein pyrogens are removed from the polymers without adversely impacting the polymers' inherent chemical structures and physical properties. PHAs with advantageous processing characteristics, including low melting points and/or solubility in non-toxic solvents, are also described. PHAs are provided which are suitable for use in in vivo applications such as in tissue coatings, stents, sutures, tubing, bone and other prostheses, bone or tissue cements, tissue regeneration devices, wound dressings, drug delivery, and for diagnostic and prophylactic uses. Properties which are selected for include degradability, elasticity, inclusion of functional groups or derivatized groups, which can in turn be used to attach targeting agents, and bioadhesion.