公开(公告)号：EP0165535A3

公开(公告)日：1987-07-15

申请号：EP85107105

申请日：1985-06-10

Applicant: MILES LABORATORIES, INC.

Inventor： Shaffer, Gerald H.

IPC: G01N21/47

CPC classification number: G01N21/474 ,  G01N2021/478 ,  G01N2201/06146 ,  G01N2201/0621 ,  G01N2201/0696

Abstract: A device for making accurate, reproducible light reflectance measurements on an optically nonuniform specimen comprises means for holding the specimen in a fixed position. In one embodiment the specimen is illuminated by light sources that are arranged at acute angles relative to the specimen and ( ± 1°) apart in azimuth, where N = the total number of light sources. The light sources are sequentially energized and deenergized to illuminate the specimen. A light detector receives light reflecting from the surface and generates an electrical signal, which is subsequently processed. In another embodimentthe light sources are arranged in pairs in which the two light sources in a pair are simultaneously energized and deenergized and the pairs are sequentially energized and deenergized. A method for measuring reflected light is also provided. The device and method are useful for making biochemical assays with reagent test strips by measuring the tone of coloration of the strips after treatment with a target substance being examined.