公开(公告)号：WO1997046264A3

公开(公告)日：1997-12-11

申请号：PCT/US1997009782

申请日：1997-06-05

Applicant: MOLECULAR BIOSYSTEMS, INC. ,  JABLONSKI, Edward, G.

Inventor： MOLECULAR BIOSYSTEMS, INC.

IPC: A61K49/00

公开(公告)号：WO1990010715A1

公开(公告)日：1990-09-20

申请号：PCT/US1990001191

申请日：1990-03-05

Applicant: MOLECULAR BIOSYSTEMS, INC.

Inventor： MOLECULAR BIOSYSTEMS, INC. ,  KERSCHNER, Jo, Anne, H. ,  JABLONSKI, Edward, G.

IPC: C12Q01/68

CPC classification number: C12Q1/701 ,  C12Q1/6841

Abstract: A process is provided for detecting nucleic acid sequences within a cell in suspension. A technique for directly hybridizing labelled deoxyoligonucleotides with cellular DNA or RNA sequences contained in suspended, intact cells is disclosed along with techniques for separating cells containing hybridized DNA or RNA from cells that do not contain hybridized DNA or RNA.

Abstract translation: 提供了用于检测悬浮细胞内的核酸序列的方法。 公开了用于将标记的脱氧寡核苷酸与悬浮的完整细胞中所含的细胞DNA或RNA序列直接杂交的技术，以及从不含杂交DNA或RNA的细胞中分离含有杂交DNA或RNA的细胞的技术。

公开(公告)号：WO1995001187A1

公开(公告)日：1995-01-12

申请号：PCT/US1994007533

申请日：1994-07-01

Applicant: MOLECULAR BIOSYSTEMS, INC.

Inventor： MOLECULAR BIOSYSTEMS, INC. ,  LAMBERT, Karel, J. ,  PODELL, Sheila, Benay ,  JABLONSKI, Edward, G. ,  HULLE, Carl ,  HAMILTON, Kenneth ,  LOHRMANN, Rolf

IPC: A61K49/00

CPC classification number: B01J13/02 ,  A61K49/223 ,  B01J13/04

Abstract: Encapsulated gas microspheres having improved pressure resistance and stability are made by: mixing an aqueous solution of a filmogenic protein such as human serum albumin with a water insoluble gas such as perfluoropropane and subjecting the mixture to ultrasonic or mechanical cavitation in the absence of oxygen in an apparatus that is closed to the atmosphere.

Abstract translation: 具有改善的耐压性和稳定性的包封气体微球通过：将成膜蛋白质如人血清白蛋白的水溶液与不溶于水的气体例如全氟丙烷混合，并在不存在氧气的情况下将混合物超声或机械空化 靠近大气的设备。

公开(公告)号：WO1994022368A1

公开(公告)日：1994-10-13

申请号：PCT/US1994002477

申请日：1994-03-09

Applicant: MOLECULAR BIOSYSTEMS, INC.

Inventor： MOLECULAR BIOSYSTEMS, INC. ,  LOHRMANN, Rolf ,  KRISHNAN, Ashwin

IPC: A61B05/055

CPC classification number: C07F9/10 ,  A61K49/06 ,  C07C17/14 ,  C07C17/26 ,  C07C45/00 ,  C07C57/58 ,  C07C69/65 ,  C07C211/29 ,  C07C229/36 ,  C07C229/42 ,  C07C237/06 ,  Y10S514/832 ,  Y10T436/24 ,  C07C25/02 ,  C07C25/13 ,  C07C47/55

Abstract: Organic compounds for diagnostic imaging which contain at least one aryl group which has been derivatized to contain at least one perfluoro-1H,1H-neopentyl moiety are provided. The perfluoro-1H, 1H-neopentyl groups produce a single magnetic resonance to insure a maximum signal to noise ratio. One compound disclosed is 2-O-oleoylglycerol 1,3-bis(7'-{3",5"-di[2"',2"'-di(trifluoromethyl)3"',3"',3"'-trifluoropropyl]phenyl}heptanoate). In the preferred embodiment, a lipid emulsion is provided as a carrier vehicle to deliver the derivitized analog to a mammalian recipient. Methods to use these compounds in MRI and computerized tomography are provided.

Abstract translation: 提供了含有至少一个衍生化为含有至少一个全氟-1H，1-新戊基部分的芳基的用于诊断成像的有机化合物。 全氟-1H，1H-新戊基产生单个磁共振以确保最大的信噪比。 公开的一种化合物是2-O-油酰甘油1,3-双（7' - {3“，5” - 二[2“'，2”' - 二（三氟甲基）3“'，3”'，3“ 三氟丙基]苯基}庚酸酯）在优选的实施方案中，提供脂质乳剂作为载体载体以将衍生的类似物递送到哺乳动物受体。提供了在MRI和计算机断层摄影中使用这些化合物的方法。

公开(公告)号：WO1983001451A1

公开(公告)日：1983-04-28

申请号：PCT/US1982001447

申请日：1982-10-08

Applicant: MOLECULAR BIOSYSTEMS, INC.

Inventor： MOLECULAR BIOSYSTEMS, INC. ,  TULLIS, Richard, H.

IPC: C07H21/02

CPC classification number: C12N15/1131 ,  A61K31/7088 ,  A61K31/7135 ,  C07H21/00 ,  C12N15/1136 ,  C12N2310/321

Abstract: For use in controlling biologic functions in an organism, a stabilized oligonucleotide, preferably in a phosphotriester form, having a base sequence substantially complementary to a portion of messenger ribonucleic acid coding for a biological component, such as a protein, of the organism. The oligonucleotide has about fourteen bases or more, such as twenty-three bases, and can be a deoxyribonucleotide. The oligonucleotide sequence can be derived from the organism's ribonucleic or deoxyribonucleic acid that codes for a vital protein, and can be synthesized in bulk either chemically or by insertion into a plasmid.

Abstract translation: 用于控制生物体中生物功能的稳定的寡核苷酸，优选磷酸三酯形式，其具有与编码生物体的生物成分（例如蛋白质）的部分信使核糖核酸基本上互补的碱基序列。 寡核苷酸具有约14个碱基或更多个碱基，例如二十三个碱基，并且可以是脱氧核糖核苷酸。 寡核苷酸序列可以衍生自编码重要蛋白质的生物体的核糖核酸或脱氧核糖核酸，并且可以通过化学方式或通过插入质粒来大量合成。

公开(公告)号：WO1996040283A1

公开(公告)日：1996-12-19

申请号：PCT/US1996009547

申请日：1996-06-07

Applicant: MOLECULAR BIOSYSTEMS, INC. ,  LOHRMANN, Rolff

Inventor： MOLECULAR BIOSYSTEMS, INC.

IPC: A61K49/00

CPC classification number: A61K49/223

Abstract: The present invention relates to a process for making insoluble gas-filled, pressure-resistant microspheres containing a liquid or solid hydrophobic barrier within the microsphere shell, and products of this process. This barrier serves to decrease the rate of gas exchange between the microsphere and the aqueous environment surrounding the microsphere and thus enhances resistance to pressure due to gas exchange.

Abstract translation: 本发明涉及一种制备在微球壳内含有液体或固体疏水屏障的不溶性气体填充的耐压微球的方法，以及该方法的产物。 该阻隔层用于降低微球体与微球体周围的水性环境之间的气体交换速率，从而提高由气体交换引起的对压力的抵抗力。

公开(公告)号：WO1996040278A3

公开(公告)日：1996-12-19

申请号：PCT/US1996008878

申请日：1996-06-04

Applicant: MOLECULAR BIOSYSTEMS, INC.

Inventor： MOLECULAR BIOSYSTEMS, INC. ,  JABLONSKI, Edward, G.

IPC: A61K49/00

公开(公告)号：WO1996014878A2

公开(公告)日：1996-05-23

申请号：PCT/US1995014801

申请日：1995-11-14

Applicant: MOLECULAR BIOSYSTEMS, INC.

Inventor： MOLECULAR BIOSYSTEMS, INC. ,  KRISHNAN, Ashwin, M. ,  LOHRMANN, Rolf

IPC: A61K49/50

CPC classification number: A61K49/126 ,  A61K49/0442 ,  A61K49/128 ,  A61K49/20 ,  Y10S514/836 ,  Y10T436/24

Abstract: Calixarene conjugates useful for imaging, particularly magnetic resonance imaging (MRI) and computed tomography (CT) are described. Said calixarene conjugates comprise (i) a calixarene backbone, and (ii) at least one imaging moiety linked thereto, and may be of formula (II) wherein at least one of the R and R substituents comprises an imaging moiety, the remaining R and R substituents are spectator groups, J is an ortho-linker, and n is an integer from 4 to 8. Imaging moieties useful for CT imaging include those comprising two or more iodine atoms. Imaging moieties useful for MRI include (i) organic moieties comprising four or more fluorine atoms; (ii) nitroxyl spin labeled moieties; and (iii) metal chelate moieties.

Abstract translation: 描述了可用于成像，特别是磁共振成像（MRI）和计算机断层摄影（CT）的杯芳烃缀合物。 所述杯芳烃缀合物包含（i）杯芳烃主链，和（ii）与其连接的至少一个成像部分，并且可以是式（II），其中R 1和R 4取代基中的至少一个包括成像 部分，剩余的R 1和R 4取代基是观察基团，J是邻位基团，n是4至8的整数。用于CT成像的成像部分包括包含两个或更多个碘原子的那些。 可用于MRI的成像部分包括（i）包含四个或更多个氟原子的有机部分; （ii）硝酰自旋标记部分; 和（iii）金属螯合物部分。

公开(公告)号：WO1986004611A1

公开(公告)日：1986-08-14

申请号：PCT/US1986000048

申请日：1986-01-14

Applicant: MOLECULAR BIOSYSTEMS, INC.

Inventor： MOLECULAR BIOSYSTEMS, INC. ,  WIDDER, Kenneth, J.

IPC: C12Q01/100

CPC classification number: C12Q1/34 ,  G01N2333/16 ,  G01N2333/978

Abstract: Blood collected for storage and subsequent transfusion is tested for AIDS transmission potential by determining whether purine salvage enzyme activities are abnormal in the red blood cells. If the adenosine deaminase (ADA) and/or the purine nucleoside phosphorylase (PNP) activities are elevated, the blood is suspect and should not be used unless other tests establish its safety.

Abstract translation: 通过测定红细胞中嘌呤补救酶活性是否异常，检测收集储存和随后输血的血液中的艾滋病传播潜力。 如果腺苷脱氨酶（ADA）和/或嘌呤核苷磷酸化酶（PNP）活性升高，血液是可疑的，不应该使用，除非其他测试确定其安全性。

公开(公告)号：WO1985001051A1

公开(公告)日：1985-03-14

申请号：PCT/US1984001351

申请日：1984-08-21

Applicant: MOLECULAR BIOSYSTEMS, INC.

Inventor： MOLECULAR BIOSYSTEMS, INC. ,  ARNOLD, Lyle, J., Jr.

IPC: C07H15/12

CPC classification number: C07H21/00 ,  Y02P20/55

Abstract: A versatile polymeric support system for the synthesis of oligonucleotides featuring a universal primer which allows chain elongation, in either the 3' or 5' direction, with any currently available DNA or RNA synthesis method, by a process which utilizes oxidatively cleaved primers to facilitate chain elongation and release. The support system is capable of withstanding mildly basic and acidic reaction conditions, while still permitting a convenient and quantitative release, either before or after removal of protecting groups from reactive groups, of synthesized oligonucleotides from a single polymeric support. Removal of the protecting groups before cleavage of the oligomer from the support permits the use of the immobilized oligomer as an affinity hybridization support for both isolation and detecting complementary polynucleic acids.

Abstract translation: 用于合成特异性通用引物的寡核苷酸的通用聚合物支持体系，其通过利用氧化裂解的引物促进链的方法，其允许以任何目前可用的DNA或RNA合成方法在3'或5'方向上链延长 伸长和释放。 支持体系能够承受轻度碱性和酸性的反应条件，同时在从单一聚合物载体除去合成的寡核苷酸的保护基团的反应性基团之前或之后仍然允许方便和定量的释放。 在从载体裂解低聚物之前去除保护基允许使用固定化的寡聚物作为分离和检测互补多核酸的亲和杂交支持。