公开(公告)号：EP0724719A4

公开(公告)日：1998-12-09

申请号：EP95924012

申请日：1995-06-20

Applicant: MOLECULAR DYNAMICS

Inventor： HUETON IAIN ,  VAN GELDER EZRA

IPC: G01N21/64 ,  G01N27/447 ,  G01N21/61

CPC classification number: G01N21/6452 ,  G01N27/44721 ,  G01N2021/6417 ,  G01N2201/101

Abstract: A high-speed fluorescence scanner for scanning a sample at equal angles has most of its optical components, including a light beam source (11), a detector (19), filters (18), lenses (17), and reflectors (15-16), in a fixed position (24), removed from the scan head (22). The lightweight scan head contains a single reflector (13) and lens (12) combination which is reciprocated rapidly along one axis to lengthen and shorten a region of the path of a collimated excitation beam (30) and to form a scan line (25-26) on a sample. The fluorescence emission is gathered by the lens (12) of the scan head and directed back, generally along the optical path of the excitation beam, to a detector. Another embodiment places the light source (43), in miniature form, directly on the scan head. The sample may be translated in an axis orthogonal to the scan line to stimulate fluorescent emission from two-dimensional portion of the sample.

Abstract translation: 用于以相等角度扫描样品的高速荧光扫描仪具有其大部分光学部件，包括光束源（11），检测器（19），滤光片（18），透镜（17）和反射镜（15- 从固定位置（24）移开，从扫描头（22）上移除。 轻便的扫描头包含单个反射器（13）和透镜（12）组合，其沿一个轴线快速往复运动，以加长和缩短准直激励光束（30）的路径区域并形成扫描线（25- 26）在样品上。 荧光发射由扫描头的透镜（12）收集并通常沿着激发光束的光路返回到检测器。 另一个实施例将微型形式的光源（43）直接放置在扫描头上。 样品可以在与扫描线正交的轴上平移以激发来自样品的二维部分的荧光发射。