公开(公告)号：JPS59130187A

公开(公告)日：1984-07-26

申请号：JP20292983

申请日：1983-10-31

Applicant: Miles Lab

Inventor： JIYOSEFUIN GUROSHIYU ,  GEERII EI UIRUSON ,  KAREN UORUUEBAA ,  KURIFUOODO OO IEERU

IPC: C12N15/09 ,  C12N1/20 ,  C12N9/28 ,  C12N9/34 ,  C12N9/54 ,  C12N9/92 ,  C12N15/00 ,  C12N15/64 ,  C12N15/75 ,  C12Q1/04 ,  C12Q1/40 ,  C12R1/07

CPC classification number: C12N9/2417 ,  C12N9/2428 ,  C12N9/54 ,  C12N9/92 ,  C12N15/64 ,  C12N15/75 ,  C12Q1/40

Abstract: A method for heterologous cloning of a gene encoding alpha -amylase, protease, amyloglucosidase or glucose isomerase. The method involves the use of a bifunctional plasmid which can replicate in both E. coli and B. subtilis, and contains at least two selectable markers. A gene fragment for the enzyme is incorporated into the plasmid, leaving at least one functional selectable marker and a nonfunctional marker, producing a mixture of recombinant hybrid plasmids and the original bifunctional plasmid. This mixture is incorporated into E. coli microorganisms and transformants selected. The transformants which contain the recombinant plasmid are identified, and plasmid DNA extracted. The plasmid DNA is used to transform Bacillus host microorganisms and transformants containing the recombinant plasmids are selected by means of the functional selectable marker.

公开(公告)号：JPS59113897A

公开(公告)日：1984-06-30

申请号：JP20292883

申请日：1983-10-31

Applicant: Miles Lab

Inventor： JIYOSEFUIN GUROSHIYU ,  GEERII EI UIRUSON ,  KAREN UORUUEBAA ,  KURIFUOODO OO IEERU

IPC: C12N1/02 ,  C12Q1/04

CPC classification number: C12Q1/04