Abstract:
There is provided a microchip, microchip controlling method and microchip controlling apparatus which have excellent flexibility in design of flow paths. The microchip comprises a plurality of laminated elastic sheets, wherein the elastic sheets are partially inadhesive each other so that at least 3 middle layers are formed; as a first middle layer, first flow path and second flow path through which liquid flows are formed; as a second middle layer, a first flow path opening/closing section for opening and closing the first flow path is formed; and as a third middle layer, a second flow path opening/closing section for opening and closing the second flow path is formed.
Abstract:
An microchip contributing to improving precision in analysis is provided, since individual identification with DNA is used in criminal investigation, thus an excellent precision in analysis is required. The microchip comprises a PCR section, a denaturing section, and an electrophoresis section. In the PCR section, a desired region in DNA is amplified. In the denaturing section, PCR amplicon of double-strand DNA is denatured into single-strand DNA. In the electrophoresis section, the PCR amplicon is separated based on the length of sequence.
Abstract:
In a sample heating method which uses a microchip, using the microchip which includes a vessel portion (70e) in which at least a portion is configured of an elastic member and a flow channel (61b) which leads a liquid sample to the vessel portion (70e), after a liquid sample is put in the vessel portion (70e), the liquid sample is heated while pressure is applied with respect to an inner portion of the vessel portion (70e).
Abstract:
The present invention provides a chip for analysis of a target substance that is compact and allows analysis of a target substance with less time and effort. The chip for analysis of a target substance includes a first flexible substrate 1, a second flexible substrate 2, and a third substrate 3. A flow channel-forming non-bonded area 11 is formed on a bonding surface of the first flexible substrate 1 and the second flexible substrate 2 in a band-like manner and an extraction chamber-forming non-bonded area 5 having a wider band width than the flow channel-forming non-bonded area 11 is formed at a part of the flow channel-forming non-bonded area 11. The first flexible substrate 1 includes a through-hole 7 that is in contact with the flow channel-forming non-bonded area 11, a shutter-forming non-bonded area 12b is formed on a bonding surface of the second flexible substrate 2 and the third substrate 3 at a far side of the through-hole 7 relative to the extraction chamber-forming non-bonded area 5 such that the shutter-forming non-bonded area 12b and the flow channel-forming non-bonded area 11 intersect above and below, the first flexible substrate 1 and the second flexible substrate 2 include a pressure supply port 18b that comes through the substrates so as to be in contact with the shutter-forming non-bonded area 12b, and a magnetic particle 16 that binds to a target substance is placed above the extraction chamber-forming non-bonded area 5.
Abstract:
PROBLEM TO BE SOLVED: To provide a fluid control mechanism with simple, inexpensive structure and high reliability in transfer means of a microchip for reacting a plurality of samples to perform reaction, mixture, separation, analysis, and gene analysis of chemical samples.SOLUTION: A fluid control mechanism of a microchip performs predetermined processing to samples, and includes; a sample part for filling the samples; first and second reaction parts for mixing and reacting the samples; a disposal part for disposing the samples or gas; a first flow channel which connects the sample part with the first reaction part; a second flow channel which connects the first reaction part with the second reaction part; and a third flow channel which connects the first reaction part with the disposal part. The first and second flow channels are provided at a lower part of the sample part, the first and second reaction parts, and the third flow channel is provided at an upper part of the first reaction part and the disposal part.