公开(公告)号：WO2005024436B1

公开(公告)日：2005-05-12

申请号：PCT/JP2004012753

申请日：2004-09-02

Applicant: NEC CORP ,  IIDA KAZUHIRO ,  TANAKA TAKASHI

Inventor： IIDA KAZUHIRO ,  TANAKA TAKASHI

IPC: B01L3/00 ,  G01N35/08 ,  B01J19/00 ,  C12M1/00 ,  C12Q1/00 ,  G01N1/10 ,  G01N27/26 ,  G01N27/447 ,  G01N31/20 ,  G01N33/48 ,  G01N37/00

CPC classification number: B01L3/502738 ,  B01L3/502707 ,  B01L3/502746 ,  B01L3/502753 ,  B01L2200/10 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0864

Abstract: An analysis chip customizable according to analysis items and a method of manufacturing the chip. Dispensing flow channels (222) branched from a main flow channel (221) and communicating with a plurality of detection tanks (223) are formed in the chip (313). Adjusting parts (314) are installed in the dispensing flow channels (222), and the opening/closing of the adjusting parts (314) is set up. By setting the adjusting parts installed in analysis parts, a variety of analysis steps can be realized.

Abstract translation: 根据分析项可定制的分析芯片和制造芯片的方法。 在芯片（313）中形成有从主流路（221）分支并与多个检测罐（223）连通的分配流路（222）。 调节部件（314）安装在分配流动通道（222）中，并且调节部件（314）的打开/关闭被设定。 通过设置分析部件中的调整部件，可以实现各种分析步骤。

公开(公告)号：CA2507376A1

公开(公告)日：2004-06-17

申请号：CA2507376

申请日：2003-11-28

Applicant: NEC CORP

Inventor： SOMEYA HIROKO ,  IGUCHI NORIYUKI ,  SANO TORU ,  KAWAURA HISAO ,  IIDA KAZUHIRO ,  ASOGAWA MINORU ,  BABA MASAKAZU ,  HATTORI WATARU

IPC: B01D67/00 ,  B01D71/02 ,  B03C5/00 ,  G01N1/40 ,  B01D57/00 ,  B01D69/00 ,  G01N37/00 ,  B01D57/02 ,  G01N27/26

Abstract: A mass spectrometry system capable of recovering specific components from a specimen in high densities and performing a solvent displacement. A separati on device (100) is installed on a microchip and incorporates a flow passage (11 2) for flowing the specific components therein. The flow passage (112) is forme d of a specimen inlet flow passage (300), a filtrate discharge flow passage (302) branched from the specimen inlet flow passage (300), and a specimen recovering part (308) branched from the specimen inlet flow passage. A filte r (304) stopping the passage of the specific components is installed at the inlet of the filtrate discharge flow passage (302) from the specimen inlet flow passage (300). A damming area (hydrophobic area) (306) stopping the ent ry of liquid specimen and allowing the liquid specimen to be passed by an external force of a specified value or more provided thereto is installed at the inlet of the specimen recovering part (308) from the specimen inlet flow passage (300).

公开(公告)号：EP1457251A4

公开(公告)日：2005-10-19

申请号：EP02775403

申请日：2002-10-28

Applicant: NEC CORP

Inventor： IIDA KAZUHIRO ,  IGUCHI NORIYUKI ,  BABA MASAKAZU ,  SOMEYA HIROKO ,  KAWAURA HISAO ,  SANO TORU ,  SAKAMOTO TOSHITSUGU

IPC: G01N33/48 ,  B01D43/00 ,  B01D57/00 ,  B01D57/02 ,  B01D61/00 ,  B01D61/18 ,  B01D61/24 ,  B01D61/28 ,  B01D63/00 ,  B01D67/00 ,  B01D69/02 ,  B01D71/02 ,  B01L3/00 ,  B03B5/00 ,  B03C5/00 ,  B81B1/00 ,  G01N1/28 ,  G01N1/38 ,  G01N27/447 ,  G01N30/60 ,  B01D69/00 ,  G01N1/10 ,  G01N30/88 ,  G01N37/00

CPC classification number: B01D67/0006 ,  B01D57/02 ,  B01D61/002 ,  B01D61/18 ,  B01D61/24 ,  B01D61/28 ,  B01D67/0034 ,  B01D67/0058 ,  B01D67/0065 ,  B01D67/0088 ,  B01D67/0093 ,  B01D69/02 ,  B01D71/02 ,  B01D71/025 ,  B01D71/027 ,  B01D2323/34 ,  B01D2325/36 ,  B01D2325/38 ,  B01L3/502753 ,  B01L3/502761 ,  B01L2200/0647 ,  B01L2200/10 ,  B01L2300/0681 ,  B01L2300/0861 ,  G01N1/4077 ,  G01N30/6069 ,  G01N30/6095 ,  G01N2001/383

Abstract: A separating device which comprises two flow route grooves separated by a partition wall and, provided between them, a plurality of separation routes which pass only the molecules having a predetermined size or less; and a separation method using the device. The separation method allows the separation of a substance having a small size, such as a cell, a nucleic acid and a protein to be carried out with a small amount of a sample, in a short time, with excellent resolution, and without causing problems of clogging and the like.

公开(公告)号：EP1413346A4

公开(公告)日：2005-05-25

申请号：EP02751824

申请日：2002-08-01

Applicant: NEC CORP

Inventor： IIDA KAZUHIRO ,  IGUCHI NORIYUKI ,  KAWAURA HISAO ,  SANO TORU ,  BABA MASAKAZU ,  SOMEYA HIROKO ,  SAKAMOTO TOSHITSUGU

IPC: B01D15/34 ,  B01L3/00 ,  B81B1/00 ,  G01N27/447 ,  G01N30/52 ,  G01N30/60 ,  B01D43/00 ,  B01D57/00 ,  G01N1/10 ,  G01N27/26 ,  G01N37/00

CPC classification number: G01N30/6095 ,  B01D15/34 ,  B01L3/502761 ,  B01L2200/12 ,  B01L2300/0681 ,  B01L2400/0415 ,  B01L2400/086 ,  G01N27/44704 ,  G01N27/44773 ,  G01N2030/524 ,  Y10T29/49002

Abstract: A separation apparatus wherein a large number of wells are formed in the inner wall of a channel, through which a sample passes, to form a sample separation region. In case of separating nucleic acids and proteins, the maximum diameter of the wells is 300 nm or less and the average interval among wells adjacent to each other is 300 nm or less.

公开(公告)号：CA2396408C

公开(公告)日：2006-03-28

申请号：CA2396408

申请日：2002-07-31

Applicant: NEC CORP

Inventor： IGUCHI NORIYUKI ,  SANO TORU ,  SAKAMOTO TOSHITSUGU ,  IIDA KAZUHIRO ,  KAWAURA HISAO ,  BABA MASAKAZU

IPC: B01L99/00 ,  B01D57/00 ,  B01L3/00 ,  C12N15/10 ,  G01N15/10 ,  G01N15/14 ,  G01N27/447 ,  G01N30/02 ,  G01N30/28 ,  G01N30/60

Abstract: A fractionating apparatus is used for fractionating sample into microstructures different in size, and includes a fractionating unit formed with a fractionating passage; the fractionating passage is defined in a groove formed in a substrate of the fractionating unit, and pillar patches (121) are formed in the groove at intervals wider than the gap among the pillar patches (121); while the sample is migrated through the fractionating passage, small-sized DNA molecules (S) are trapped in the pillar patches (121), and large-sized DNA molecules (L) are smoothly migrated through the wide intervals; this results in that the large-sized DNA molecules (L) reaches the end of the fractionati ng passage faster than the small-sized DNA molecules without clogging.

公开(公告)号：CA2508456A1

公开(公告)日：2004-06-17

申请号：CA2508456

申请日：2003-12-02

Applicant: NEC CORP

Inventor： HATTORI WATARU ,  SANO TORU ,  IIDA KAZUHIRO ,  IGUCHI NORIYUKI ,  BABA MASAKAZU ,  SOMEYA HIROKO ,  ASOGAWA MINORU ,  KAWAURA HISAO

IPC: B01L3/00 ,  G01N30/20 ,  H01J49/04 ,  G01N1/00 ,  B81C1/00 ,  B01D57/00 ,  G01N37/00 ,  B01D57/02 ,  G01N35/08 ,  H01J49/26 ,  G01N27/447 ,  G01N33/48 ,  G01N30/60 ,  G01N27/62 ,  G01N30/72

Abstract: A liquid sample (104) introduced in a main flow passage (101) is held in a d am portion (105), and a trigger liquid (106) is filled in a trigger flow passag e (102). In this state, the trigger liquid (106) is further introduced at desired timing into the trigger flow passage (102) so that the front end portion of the level of the trigger liquid (106) is advanced and the front e nd portion is brought to be into contact with the dam portion (105). This cause s the liquid sample (104) to move to the right (downstream side) in the figure , resulting in the liquid sample (104) flowing out to the downstream side of t he main flow passage (101). This means that the trigger liquid (106) provides priming to realize a liquid switch.

公开(公告)号：CA2436354C

公开(公告)日：2007-04-10

申请号：CA2436354

申请日：2002-08-01

Applicant: NEC CORP

Inventor： IIDA KAZUHIRO ,  IGUCHI NORIYUKI ,  SAKAMOTO TOSHITSUGU ,  SOMEYA HIROKO ,  BABA MASAKAZU ,  KAWAURA HISAO ,  SANO TORU

IPC: B01D43/00 ,  B01D15/34 ,  B01D57/00 ,  B01L3/00 ,  B81B1/00 ,  G01N1/10 ,  G01N27/26 ,  G01N27/447 ,  G01N30/52 ,  G01N30/60 ,  G01N37/00

Abstract: A separator has a specimen separating area comprising a number of recesses defined in an inner wall of a flow passage through which a specimen passes. For separating nucleic acid and protein, the recesses have openings with a maximum diameter of 300 nm, and are spaced apart at an average interval of 300 nm or less.

公开(公告)号：CA2506562A1

公开(公告)日：2004-06-17

申请号：CA2506562

申请日：2003-11-28

Applicant: NEC CORP

Inventor： IGUCHI NORIYUKI ,  KAWAURA HISAO ,  SOMEYA HIROKO ,  IIDA KAZUHIRO ,  HATTORI WATARU ,  SANO TORU ,  ASOGAWA MINORU ,  BABA MASAKAZU

IPC: G01N1/40 ,  H01J49/04 ,  G01N1/28 ,  B01D57/00 ,  B01D57/02 ,  B81C1/00 ,  G01N27/447 ,  G01N27/62 ,  G01N30/60 ,  G01N30/72 ,  G01N33/48 ,  G01N35/08 ,  G01N37/00 ,  H01J49/26

Abstract: A substrate (101) is provided with a channel (103), and a drying section (10 7) having many columnar bodies (105) is formed at one end of the channel (103). The top of the channel (103) except the portion above the drying section (10 7) is covered with a covering (109). A sample supplied into the channel (103) moves to the drying section (107) due to capillary phenomenon. The drying section (107) is heated with a heater (111), so that the solvent is evaporated, thereby concentrating and drying the solute.

公开(公告)号：CA2436354A1

公开(公告)日：2003-02-20

申请号：CA2436354

申请日：2002-08-01

Applicant: NEC CORP

Inventor： SAKAMOTO TOSHITSUGU ,  BABA MASAKAZU ,  IGUCHI NORIYUKI ,  SOMEYA HIROKO ,  KAWAURA HISAO ,  SANO TORU ,  IIDA KAZUHIRO

IPC: B01D15/34 ,  B01L3/00 ,  B81B1/00 ,  G01N27/447 ,  G01N30/52 ,  G01N30/60 ,  B01D43/00 ,  B01D57/00 ,  G01N37/00 ,  G01N1/10 ,  G01N27/26

Abstract: A separation apparatus wherein a large number of wells are formed in the inn er wall of a channel, through which a sample passes, to form a sample separatio n region. In case of separating nucleic acids and proteins, the maximum diamet er of the wells is 300 nm or less and the average interval among wells adjacent to each other is 300 nm or less.

公开(公告)号：CA2396408A1

公开(公告)日：2003-02-03

申请号：CA2396408

申请日：2002-07-31

Applicant: NEC CORP

Inventor： IIDA KAZUHIRO ,  KAWAURA HISAO ,  SAKAMOTO TOSHITSUGU ,  BABA MASAKAZU ,  SANO TORU ,  IGUCHI NORIYUKI

IPC: B01L99/00 ,  B01L3/00 ,  G01N15/10 ,  G01N15/14 ,  G01N27/447 ,  G01N30/02 ,  G01N30/28 ,  G01N30/60 ,  B01L11/00 ,  B01D57/00 ,  C12N15/10

Abstract: A fractionating apparatus is used for fractionating sample into microstru c- tures different in size, and includes a fractionating unit formed with a fra c- tionating passage; the fractionating passage is defined in a groove formed i n a substrate of the fractionating unit, and pillar patches (121) are formed in the groove at intervals wider than the gap among the pillar patches (121); while the sample is migrated through the fractionating passage, small-sized DNA molecules (S) are trapped in the pillar patches (121), and large-sized DNA molecules (L) are smoothly migrated through the wide intervals; this results in that the large-sized DNA molecules (L) reaches the end of the fractionati ng passage faster than the small-sized DNA molecules without clogging.