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    ON-LINE LIQUID SAMPLE DEPOSITION INTERFACE FOR MATRIX ASSISTED LASER DESORPTION IONIZATION-TIME OF FLIGHT (MALDI-TOF) MASS SPECTROSCOPY
    2.
    发明公开
    ON-LINE LIQUID SAMPLE DEPOSITION INTERFACE FOR MATRIX ASSISTED LASER DESORPTION IONIZATION-TIME OF FLIGHT (MALDI-TOF) MASS SPECTROSCOPY 失效
    接口液P *罗伯特·矩阵式协助，-DESORPTIONSFLUGZEITMASSENSPEKTROMETRIE激光电离ON-LINE DROP

    公开(公告)号：EP0986746A1

    公开(公告)日：2000-03-22

    申请号：EP98924853.9

    申请日：1998-05-22

    Applicant: NORTHEASTERN UNIVERSITY

    Inventor： KARGER, Barry, L. ,  FORET, Frantisek ,  PREISLER, Jan

    IPC: G01N24/00 ,  G01N27/64 ,  G01N1/22 ,  B01D59/44 ,  H01J49/00

    CPC classification number: H01J49/0418 ,  H01J49/0431

    Abstract: An universal interface for continuous on-line liquid sample introduction directly into the source chamber (5) of a time-of-flight mass spectrometer (20) for matrix-assisted-laser-desorption-ionization (MALDI) analysis is disclosed. The liquid sample includes a liquid or solid matrix material used in conventional MALDI analysis and is deposited directly onto a moving sample holder (22) in the source chamber (5). The sample holder (22) is at subatmospheric pressure, causing the rapid drying of the sample. The dried sample is subsequently desorbed from the sample holder (22) by a nitrogen laser (28) in a desorption region (26). The method of the invention is particularly amenable to multiplexing, the parallel deposition of multiple samples from an array of capillaries or microchip channels (24), with the subsequent sequential desorption by a scanning laser. This format is particularly useful for high throughput mass spectrometric analysis.

    Abstract translation: 对于连续在线液体样品引入直接进入时间飞行质谱仪（20），用于基质辅助激光解吸电离质谱（MALDI）分析的源室（5）的通用接口被游离缺失盘。 所述液体样品包含常规MALDI分析中使用的液体或固体基质材料和直接沉积到在源室（5）的移动样本保持器（22）。 样本保持器（22）是在低于大气压的压力，从而导致样品的快速干燥。 干燥的样品随后从样品架（22）通过一个氮激光器（28）到解吸区（26）脱离。 本发明的方法是特别适合于复用，多个样品的平行沉积从毛细管或微芯片通道（24）的阵列，其中通过扫描激光的后续顺序解吸。 此格式适用于高通量质谱分析特别有用。

    SILOXANEDIOL COATING FOR CAPILLARY ELECTROPHORESIS AND FOR GENERAL SURFACE MODIFICATION
    3.
    发明申请
    SILOXANEDIOL COATING FOR CAPILLARY ELECTROPHORESIS AND FOR GENERAL SURFACE MODIFICATION 审中-公开
    用于毛细管电泳和一般表面改性的硅氧烷涂层

    公开(公告)号：WO1994015209A1

    公开(公告)日：1994-07-07

    申请号：PCT/US1993012513

    申请日：1993-12-22

    Applicant: NORTHEASTERN UNIVERSITY

    Inventor： NORTHEASTERN UNIVERSITY ,  KARGER, Barry, L. ,  SCHMALZING, Dieter ,  FORET, Frantisek

    IPC: G01N27/447

    CPC classification number: G01N27/44752

    Abstract: A coated microcapillary column for high performance electrophoresis is disclosed. A preferred microcapillary includes a column; a universal sub-layer of coating material that has been highly cross-linked using a cross-linking agent and attached covalently or non-covalently to the column wall; and a variable top layer of a monomer or polymer, preferably cross-linked, attached to the sub-layer. The microcapillary preferably is prepared by covalently bonding a highly cross-linked siloxanediol sub-layer to the inner surface of the microcapillary wall and then causing a mixture of monomers or polymers to react in the bore of the microcapillary to form a top layer. The bilayer coating as used in a microcapillary prevents adsorption of solutes during electrophoresis and eliminates or controls electroosmotic flow. The disclosed coating may also be formed on other kinds of surfaces where similar surface modification is desired. The sub-layer, which can be formed from other siloxane derivatives having two nucleophilic end groups, e.g., diamino or dithiol, may be used independently, or as a base layer for bilayer or even multilayer surface formation.

    Abstract translation: 公开了一种用于高性能电泳的涂层微毛细管柱。 优选的微毛细管包括柱; 已使用交联剂高度交联并与塔壁共价或非共价附着的通用的涂层材料层; 以及连接到子层上的优选交联的单体或聚合物的可变顶层。 微毛细管优选通过将高度交联的硅氧烷二醇亚层共价键合到微毛细管壁的内表面，然后使单体或聚合物的混合物在微毛细孔的孔中反应以形成顶层来制备。 在微毛细管中使用的双层涂层防止电泳期间溶质的吸附，并消除或控制电渗流。 所公开的涂层也可以形成在需要类似表面改性的其它种类的表面上。 可以由具有两个亲核端基的其它硅氧烷衍生物形成的亚层，例如二氨基或二硫醇，可以独立使用，或作为双层或甚至多层表面形成的基层使用。

    SUBATMOSPHERIC, VARIABLE PRESSURE SAMPLE DELIVERY CHAMBER FOR ELECTROSPRAY IONIZATION/MASS SPECTROMETRY AND OTHER APPLICATIONS
    4.
    发明公开
    SUBATMOSPHERIC, VARIABLE PRESSURE SAMPLE DELIVERY CHAMBER FOR ELECTROSPRAY IONIZATION/MASS SPECTROMETRY AND OTHER APPLICATIONS 失效
    带可变压力电/ MASENSPEKTROMETRIE和其他应用程序样品供应BOARD

    公开(公告)号：EP0888169A1

    公开(公告)日：1999-01-07

    申请号：EP97903835.0

    申请日：1997-01-17

    Applicant: NORTHEASTERN UNIVERSITY

    Inventor： KARGER, Barry, L. ,  FORET, Frantisek ,  KIRBY, Daniel, P.

    IPC: G01N27 ,  H01J49 ,  G01N30

    CPC classification number: H01J49/045 ,  G01N27/44717 ,  G01N30/7266 ,  G01N2030/285 ,  H01J49/165

    Abstract: A subatmospheric, variable pressure sample delivery chamber (2), useful as an electrospray ionization device for introducing a sample into a mass spectrometer (9) or as a sample delivery device for delivery of a sample to a collection device (74) at subatmospheric pressures, is disclosed. The sample delivery chamber (2) is configured to maintain an operating pressure between 1 and 750 Torr, preferably between 50 and 700 Torr. The chamber has an inlet port (4) for introduction of a gas and an exit port (6) capable of being coupled either to a sampling orifice (8) for an analytical device, usually a mass spectrometer (9), or directly to a pump for removal of gas. A sample delivery device (22) extends from outside the chamber into the interior of the chamber (2). The inlet end (12) of the sample delivey device is capable of receiving a sample and the exit end of the sample delivery device (14) is aligned with the exit port (6) of the chamber, if the sample is to be delivered to an external analytical device, or aligned with the collection device (74), for direct collection of the sample.

    MICROFABRICATED HYBRID CAPILLARY ARRAY AND MULTICHANNEL DETECTION ASSEMBLY
    5.
    发明申请
    MICROFABRICATED HYBRID CAPILLARY ARRAY AND MULTICHANNEL DETECTION ASSEMBLY 审中-公开
    微型混合毛细管阵列和多通道检测装置

    公开(公告)号：WO1998010122A1

    公开(公告)日：1998-03-12

    申请号：PCT/US1997015461

    申请日：1997-09-03

    Applicant: NORTHEASTERN UNIVERSITY

    Inventor： NORTHEASTERN UNIVERSITY ,  KARGER, Barry, L. ,  LIU, Shaorong ,  FORET, Frantisek ,  XUE, Quifeng

    IPC: C25B09/00

    CPC classification number: G01N27/44717 ,  G01N27/44721 ,  G01N27/44743 ,  G01N27/44791

    Abstract: A hybrid microfabricated substrate capillary array assembly, which provides an interface between the integrated channels (12) on a microfabricated substrate (10), e.g., microchip, and flexible capillaries (18), is disclosed. The hybrid device permits, e.g., convenient injection of samples from a capillary array (18) into channels (12) in a microchip (10) and also enables, e.g., convenient detection on the device by LIF (laser induced fluorescence). With the use of such an assembly, large numbers of samples may be processed simultaneously, leading to high-speed, high-throughput analyses.

    Abstract translation: 公开了一种混合微制造的基底毛细管阵列组件，其提供微加工基板（10）上的集成通道（12），例如微芯片和柔性毛细管（18）之间的界面。 混合装置允许例如将样品从毛细管阵列（18）方便地注入到微芯片（10）中的通道（12）中，并且还能够例如通过LIF（激光诱导荧光）在装置上方便地检测。 通过使用这种组件，可以同时处理大量样品，从而实现高速，高通量分析。

    SUBATMOSPHERIC, VARIABLE PRESSURE SAMPLE DELIVERY CHAMBER FOR ELECTROSPRAY IONIZATION/MASS SPECTROMETRY AND OTHER APPLICATIONS
    6.
    发明申请
    SUBATMOSPHERIC, VARIABLE PRESSURE SAMPLE DELIVERY CHAMBER FOR ELECTROSPRAY IONIZATION/MASS SPECTROMETRY AND OTHER APPLICATIONS 审中-公开
    用于电喷雾离子/质谱分析和其他应用的可变压力样品输送室

    公开(公告)号：WO1997026072A1

    公开(公告)日：1997-07-24

    申请号：PCT/US1997000733

    申请日：1997-01-17

    Applicant: NORTHEASTERN UNIVERSITY

    Inventor： NORTHEASTERN UNIVERSITY ,  KARGER, Barry, L. ,  FORET, Frantisek ,  KIRBY, Daniel, P.

    IPC: B01D59/44

    CPC classification number: H01J49/045 ,  G01N27/44717 ,  G01N30/7266 ,  G01N2030/285 ,  H01J49/165

    Abstract: A subatmospheric, variable pressure sample delivery chamber (2), useful as an electrospray ionization device for introducing a sample into a mass spectrometer (9) or as a sample device at subatmospheric pressures, is disclosed. The sample delivery chamber (2) is configured to maintain an operating pressure between 1 and 750 Torr, preferably between 50 and 700 Torr. The chamber has an inlet port (4) for introduction of a gas and an exit port (6) capable of being coupled either to a sampling orifice (8) for an analytical device, usually a mass spectrometer (9), or directly to a pump for removal of gas. A sample delivery device (22) is capable of receiving a sample and the exit port (6) of the chamber, if the sample is to be delivered to an external analytical device, or aligned with the collection device, for direct collection of the sample.

    Abstract translation: 公开了一种低于大气压的可变压力样品输送室（2），其用作将样品引入质谱仪（9）或作为低于大气压的样品装置的电喷雾离子化装置。 样品输送室（2）被构造成将操作压力保持在1和750托之间，优选在50和700托之间。 腔室具有用于引入气体的入口（4）和能够耦合到用于分析装置（通常为质谱仪（9））的采样孔（8）的出口（6），或直接连接到 泵用于除气。 样品输送装置（22）能够接收样品和室的出口（6），如果要将样品输送到外部分析装置或与收集装置对准，以便直接收集样品 。

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