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    Preparation method for sample feces, extraction method for nucleic acid or protein using preparation method, detection method for nucleic acid or protein using extraction method, detection method for cancer or infectious disease using sample feces in detection method, and preparation container for sample feces
    2.
    发明专利
    Preparation method for sample feces, extraction method for nucleic acid or protein using preparation method, detection method for nucleic acid or protein using extraction method, detection method for cancer or infectious disease using sample feces in detection method, and preparation container for sample feces 审中-公开
    用于样品FECES的制备方法，使用制备方法的核酸或蛋白质的提取方法，使用提取方法的核酸或蛋白质的检测方法，使用检测方法中的样品检测方法的癌症或感染性疾病的检测方法，以及样品FECES的制备容器

    公开(公告)号：JP2009115658A

    公开(公告)日：2009-05-28

    申请号：JP2007289895

    申请日：2007-11-07

    Applicant: Olympus Corp ,  オリンパス株式会社

    Inventor： NAKAJIMA KAZUE ,  AKESAKA TAMAO ,  FUKUOKA TAKAHISA ,  NAGAOKA TOMONORI

    IPC: G01N1/28 ,  C12M1/26 ,  C12N15/09 ,  C12Q1/06 ,  C12Q1/24 ,  C12Q1/68 ,  G01N1/04 ,  G01N33/48

    Abstract: PROBLEM TO BE SOLVED: To provide a preparation method of excellent storage stability and handleability for sample feces, capable of preventing a nucleic acid in the sample feces from being decomposed or a protein thereof from being modified or the like, allowing efficient safe extraction from the sample feces, while reducing the risk of contamination and infection to the utmost, and allowing precise and highly sensitive detection, an extraction method or the like for the nucleic acid or protein using the preparation method, and a preparation container for the sample feces.
    SOLUTION: In this preparation method for the sample feces, the collected sample feces are formed into an easily crushable shape such as a plate-like shape having 0.5-5 mm of thickness, a fibrous shape having 0.5-5 mm of diameter and a granular shape having 1-10 mm of diameter, to be freezed thereafter. This preparation container for the sample feces has a cylindrical member 11 deformable elastically and provided with a plurality of recesses 11a1 for collecting feces, on a side face, a removing means 12 for removing excessive feces E out of the feces collected in the recesses 11a1 of the cylindrical member 11, and an extrusion member 13 capable of press-deforming the recesses 11a1 by insertion thereof from an inner side of the cylindrical member 11.
    COPYRIGHT: (C)2009,JPO&INPIT

    Abstract translation: 要解决的问题：为了提供能够防止样品粪便中的核酸分解或其蛋白质被改性等的，对于样品粪便具有优异的储存稳定性和可操作性的制备方法，允许有效的安全性 从样品粪便中提取，同时最大限度地降低污染和感染的风险，并且允许精确和高度灵敏的检测，使用制备方法的核酸或蛋白质的提取方法等，以及样品的制备容器 屎。 解决方案：在这种样品粪便的制备方法中，将收集的样品粪便形成为具有0.5-5mm厚度的板状形状的容易破碎的形状，具有0.5-5mm直径的纤维形状 并且具有1-10mm直径的颗粒形状，此后被冷冻。 用于样品粪便的该制备容器具有可弹性变形的圆柱形构件11，并且在侧面上设置有用于收集粪便的多个凹槽11a1，用于从收集在凹槽11a1中的粪便中除去多余的粪便E的去除装置12 圆柱形构件11和挤压构件13，其能够通过从圆筒构件11的内侧插入而使凹部11a1压缩变形。（C）2009年，JPO＆INPIT

    Feces treatment container and method
    3.
    发明专利
    Feces treatment container and method 审中-公开
    FECES处理容器和方法

    公开(公告)号：JP2010008106A

    公开(公告)日：2010-01-14

    申请号：JP2008164961

    申请日：2008-06-24

    Applicant: Olympus Corp ,  オリンパス株式会社

    Inventor： NAKAJIMA KAZUE ,  SHIBAZAKI TAKAMI ,  NAKAMOTO MARI ,  FUKUOKA MORINAO ,  NAGAOKA TOMONORI

    IPC: G01N1/04 ,  G01N1/10

    Abstract: PROBLEM TO BE SOLVED: To provide a feces treatment method and a feces treatment container capable of performing stable nucleic acid recovery. SOLUTION: In this feces treatment container 1 equipped with a feces sampling tool 100, a suspension holding part 110 and a treating liquid holding part 120, wherein a feces sample preparing solution S is stored in a solution holding container 121, a feces sample E collected by the feces sampling tool 100 is adjusted into a fixed amount by scraping off excessive feces by a guide part 112 when the feces sampling tool 100 is fitted to a feces holding part 110. Thereafter, by pressing the feces sample E by a piston 103, the feces sample E is shredded and dispersed into feces holding container 111 by a dispersion member 113. By pressing the feces holding container 111 into the solution holding container 121, a projection part 115 drills and breaks a sealing film 122, to thereby mix the dispersed feces sample E with the feces sample preparing solution S. The feces sample E is shredded and dispersed, to thereby enable stable nucleic acid recovery. COPYRIGHT: (C)2010,JPO&INPIT

    Abstract translation: 待解决的问题：提供能够进行稳定的核酸回收的粪便处理方法和粪便处理容器。 解决方案：在具有粪便取样工具100的悬浮液保持部110和处理液保持部120的粪便处理容器1中，将粪便试样调制溶液S储存在溶液保持容器121中，将粪便 当粪便采样工具100装配到粪便保持部分110时，通过将粪便采样工具100收集的样品E调整为固定量，通过引导部分112刮除多余的粪便。之后，通过将粪便样本E 活塞103将粪便样品E切碎并通过分散构件113分散在粪便保持容器111中。通过将粪便保持容器111压入溶液保持容器121中，突出部分115钻并破坏密封膜122，由此 将分散的粪便样品E与粪便样品制备溶液S混合。将粪便样品E切碎并分散，从而使稳定的核酸回收。 版权所有（C）2010，JPO＆INPIT

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