公开(公告)号：US20180298324A1

公开(公告)日：2018-10-18

申请号：US15573459

申请日：2016-05-12

Applicant: ON-CHIP BIOTECHNOLOGIES CO., LTD.

Inventor： Kazuo TAKEDA ,  Yuu FUJIMURA ,  Takahide INO ,  Masayuki ISHIGE ,  Jin AKAGI

IPC: C12M1/00 ,  C12M1/34 ,  C12M1/32 ,  C12M1/26 ,  C12Q1/6881 ,  G01N15/14

Abstract: An object of the present invention is to purify and concentrate differentiating cells derived from ES cells, iPS cells, or the like without damaging them.The above problem can be solved by an apparatus for analyzing and separating particles comprising: a flow path cartridge, an illumination unit, a detection unit for detecting particles of interest, a force generating unit,wherein a sample liquid reservoir (sample reservoir) connected to a first flow path; a fourth branched flow path and a fifth branched flow path which are connected to the first flow path; a third-A reservoir connected to the fourth branched flow path; a third-B reservoir connected to the fifth branched flow path; and a fourth reservoir for reserving particles which are not sorting; are formed on the cartridge, and each reservoir comprise a means which equalizes an air pressure in the each reservoir with an air pressure of an in-device air pressure control system, and a stream of the flow path in the cartridge is controlled by controlling the air pressure in the each reservoir through the each in-device air pressure control system.

公开(公告)号：US20220062905A1

公开(公告)日：2022-03-03

申请号：US17445789

申请日：2021-08-24

Applicant: ON-CHIP BIOTECHNOLOGIES CO., LTD.

Inventor： Kazuo TAKEDA ,  Masayuki ISHIGE ,  Yuu FUJIMURA ,  Takahide INO ,  Yuji MORISHITA ,  Kosuke OSAWA ,  Soichiro TSUDA

IPC: B01L3/00 ,  G01N1/30 ,  G01N15/14

Abstract: One object of the present invention is to provide a method or apparatus for purifying target particles from high-concentration particles in a short time. The above problem can be solved by a method for purifying target particles, characterized in that the method comprises a step of sorting the target particles from a high concentration of non-target particles, wherein the sorting step is repeated for three times or more.

公开(公告)号：US20250093253A1

公开(公告)日：2025-03-20

申请号：US18903567

申请日：2024-10-01

Applicant: ON-CHIP BIOTECHNOLOGIES CO., LTD.

Inventor： Kazuo TAKEDA ,  Yuu FUJIMURA ,  Masayuki ISHIGE ,  Takahide INO ,  Yohsuke BANSHO ,  Jin AKAGI ,  Kosuke OSAWA

IPC: G01N15/1404 ,  B01L3/02 ,  G01N15/1433

Abstract: The object of the present invention is to provide a technique for dispensing cells as single cells. The above problem can be solved by an apparatus for dispensing particles comprising a transparent hollow pipette for dispensing sample liquid containing particles, and an image capturing means, wherein the devise has means for capturing two or more images of the dispensing liquid in the hollow pipette; means for comparing two or more shot images, distinguishing a moved particle-like substance from an unmoved particle-likesubstance in the image-captured particle-like substances, and identifying the moved particle-like substance as a suspended particle; and means for dispensing a sample liquid containing a target number of suspended particles.

公开(公告)号：US20240091775A1

公开(公告)日：2024-03-21

申请号：US18521841

申请日：2023-11-28

Applicant: ON-CHIP BIOTECHNOLOGIES CO., LTD.

Inventor： Kazuo TAKEDA ,  Masayuki ISHIGE ,  Yuu FUJIMURA ,  Takahide INO ,  Yuji MORISHITA ,  Kosuke OSAWA ,  Soichiro TSUDA

IPC: B01L3/00 ,  G01N1/30 ,  G01N15/14

CPC classification number: B01L3/502784 ,  B01L3/502715 ,  G01N1/30 ,  G01N15/1404 ,  G01N15/1484 ,  B01L2200/0673 ,  B01L2400/0487 ,  G01N2015/149

Abstract: One object of the present invention is to provide a method or apparatus for purifying target particles from high-concentration particles in a short time. The above problem can be solved by a method for purifying target particles, characterized in that the method comprises a step of sorting the target particles from a high concentration of non-target particles, wherein the sorting step is repeated for three times or more.

公开(公告)号：US20230383240A1

公开(公告)日：2023-11-30

申请号：US18450006

申请日：2023-08-15

Applicant: ON-CHIP BIOTECHNOLOGIES CO., LTD.

Inventor： Kazuo TAKEDA ,  Yuu FUJIMURA ,  Takahide INO ,  Masayuki ISHIGE ,  Jin AKAGI

IPC: C12M1/00 ,  C12N5/07 ,  C12Q1/02 ,  G01N15/14 ,  B01L3/00 ,  C12M1/32 ,  C12M1/26 ,  C12M1/34 ,  C12Q1/6881

CPC classification number: C12M47/04 ,  G01N2015/1006 ,  C12M1/00 ,  C12Q1/02 ,  G01N15/1484 ,  B01L3/502784 ,  B01L3/502761 ,  C12M23/12 ,  C12M33/04 ,  C12M41/46 ,  C12M47/06 ,  C12Q1/6881 ,  G01N15/1404 ,  G01N15/1434 ,  B01L2200/0652 ,  B01L2300/0861 ,  C12N5/06

Abstract: An object of the present invention is to purify and concentrate differentiating cells derived from ES cells, iPS cells, or the like without damaging them.
The above problem can be solved by an apparatus for analyzing and separating particles comprising: a flow path cartridge, an illumination unit, a detection unit for detecting particles of interest, a force generating unit,



wherein a sample liquid reservoir (sample reservoir) connected to a first flow path; a fourth branched flow path and a fifth branched flow path which are connected to the first flow path; a third-A reservoir connected to the fourth branched flow path; a third-B reservoir connected to the fifth branched flow path; and a fourth reservoir for reserving particles which are not sorting; are formed on the cartridge, and each reservoir comprise a means which equalizes an air pressure in the each reservoir with an air pressure of an in-device air pressure control system, and a stream of the flow path in the cartridge is controlled by controlling the air pressure in the each reservoir through the each in-device air pressure control system.

公开(公告)号：US20190339189A1

公开(公告)日：2019-11-07

申请号：US16333528

申请日：2017-09-15

Applicant: ON-CHIP BIOTECHNOLOGIES CO., LTD.

Inventor： Kazuo TAKEDA ,  Yuu FUJIMURA ,  Masayuki ISHIGE ,  Takahide INO ,  Yohsuke BANSHO ,  Jin AKAGI ,  Kosuke OSAWA

IPC: G01N15/14 ,  B01L3/02

Abstract: The object of the present invention is to provide a technique for dispensing cells as single cells. The above problem can be solved by an apparatus for dispensing particles comprising a transparent hollow pipette for dispensing sample liquid containing particles, and an image capturing means, wherein the devise has means for capturing two or more images of the dispensing liquid in the hollow pipette; means for comparing two or more shot images, distinguishing a moved particle-like substance from an unmoved particle-like substance in the image-captured particle-like substances, and identifying the moved particle-like substance as a suspended particle; and means for dispensing a sample liquid containing a target number of suspended particles.