公开(公告)号：WO2013056241A3

公开(公告)日：2013-06-20

申请号：PCT/US2012060275

申请日：2012-10-15

Applicant: PACIFIC BIOSCIENCES CALIFORNIA

Inventor： KORLACH JONAS ,  TURNER STEPHEN ,  SUN LEI

IPC: C12Q1/68 ,  C12Q1/48 ,  G01N27/26

CPC classification number: C12Q1/6869 ,  B82Y15/00 ,  C12Q1/001 ,  C12Q2521/543 ,  C12Q2563/113 ,  C12Q2565/607 ,  C40B60/10 ,  G01N27/00 ,  G01N27/3277 ,  G01N27/3278 ,  C12Q2563/155

Abstract: Real time redox sequencing methods, devices, and systems are described. Arrays of redox devices comprising one or two electrodes are used to provide sequence information about a template nucleic acid in a polymerase-template complex bound proximate to the electrode(s). A sequencing reaction mixture comprising nucleotide analogs comprising redox labels is introduced to the array of redox devices under conditions of polymerase mediated nucleic acid synthesis. The time sequence of incorporation of nucleotide analogs is determined by electrochemically identifying the redox labels of the nucleotide analogs that are incorporated into the growing strand.

Abstract translation: 描述了实时氧化还原测序方法，设备和系统。 使用包含一个或两个电极的氧化还原装置的阵列来提供关于靠近电极的聚合酶 - 模板复合物中的模板核酸的序列信息。 将包含氧化还原标记的核苷酸类似物的测序反应混合物在聚合酶介导的核酸合成的条件下引入到氧化还原装置阵列中。 通过电化学鉴定掺入生长链中的核苷酸类似物的氧化还原标记来确定核苷酸类似物的掺入时间序列。