公开(公告)号：EP0645187A2

公开(公告)日：1995-03-29

申请号：EP94111503.2

申请日：1994-07-22

Applicant: PACKARD INSTRUMENT COMPANY, INC.

Inventor： Kolb, Alfred J. ,  Manns, Roy L. ,  Neumann, Kenneth E.

IPC: B01L3/00 ,  G01T1/204 ,  G01N21/76 ,  G01T7/02

CPC classification number: B01L3/50255 ,  G01T1/204 ,  G01T7/02 ,  Y10S435/809

Abstract: A microplate assembly (10) for use in analyzing samples captured on a filter medium (16) comprises a carrier plate (12), a holding tray (14), a collimator (18), and a cover film (20). These elements are generally rectangular in shape and are sized to stack on top of one another. The holding tray (14) is positioned within the carrier plate (12), the collimator (18) and filter medium (16) are positioned within the holding tray (14) with the filter medium (16) positioned beneath the collimator (18), and the cover film (20) is sealed over the collimator (18). To prepare samples in the microplate assembly (10) for analysis, the samples are captured on the filter medium (16) and the filter medium (16) is placed in the holding tray (14). After adding scintillation cocktail or luminescent substrate to the filter medium (16), the collimator (18) is placed over the holding tray (14) with the filter medium (16) positioned between the collimator (18) and the holding tray (14) and the samples disposed in the sample wells. The carrier plate (12), the holding tray (14), the filter medium (16), and the collimator (18) are provided with complementary keyed corners (22,34,19,46) to facilitate alignment of these elements relative to one another. The wells of the collimator (18) include respective lower rims (54) protruding into the filter medium (16) to minimize crosstalk through the filter medium (16). The cover film (20) seals the microplate assembly (10) so that the samples are prepared for analysis.

Abstract translation: 用于分析在过滤介质上捕获的样品的微板组件包括载体板，保持盘，准直器和覆盖膜。 这些元件的形状通常为矩形，并且尺寸设计成彼此堆叠。 保持托盘定位在承载板内，准直器和过滤介质位于保持托盘内，过滤介质定位在准直器下方，并且覆盖膜被密封在准直器上。 为了在微板组件中制备样品用于分析，将样品捕获在过滤介质上，并将过滤介质置于保持盘中。 在过滤介质中加入闪烁混合物或发光底物之后，将准直器放置在保持盘上方，过滤介质位于准直器和保持托盘之间，样品置于样品孔中。 承载板，保持托盘，过滤介质和准直器设置有互补的键角，以便于这些元件相对于彼此对准。 准直器的阱包括突出到过滤介质中的相应的下边缘，以最小化通过过滤介质的串扰。 覆盖膜密封微板组件，使得样品准备用于分析。

公开(公告)号：EP0571661B1

公开(公告)日：1996-02-14

申请号：EP92119631.7

申请日：1992-11-17

Applicant: Packard Instrument Company, Inc.

Inventor： Manns, Roy L. ,  Kolb, Alfred J. ,  Effertz, Bernard S.

IPC: B01L3/00 ,  G01N21/03

CPC classification number: B01L3/5085 ,  B29C45/16 ,  G01N21/03 ,  G01N2021/0346

公开(公告)号：EP0645187A3

公开(公告)日：1995-10-25

申请号：EP94111503.2

申请日：1994-07-22

Applicant: PACKARD INSTRUMENT COMPANY, INC.

Inventor： Kolb, Alfred J. ,  Manns, Roy L. ,  Neumann, Kenneth E.

IPC: B01L3/00 ,  G01T1/204 ,  G01N21/76 ,  G01T7/02

CPC classification number: B01L3/50255 ,  G01T1/204 ,  G01T7/02 ,  Y10S435/809

Abstract: A microplate assembly (10) for use in analyzing samples captured on a filter medium (16) comprises a carrier plate (12), a holding tray (14), a collimator (18), and a cover film (20). These elements are generally rectangular in shape and are sized to stack on top of one another. The holding tray (14) is positioned within the carrier plate (12), the collimator (18) and filter medium (16) are positioned within the holding tray (14) with the filter medium (16) positioned beneath the collimator (18), and the cover film (20) is sealed over the collimator (18). To prepare samples in the microplate assembly (10) for analysis, the samples are captured on the filter medium (16) and the filter medium (16) is placed in the holding tray (14). After adding scintillation cocktail or luminescent substrate to the filter medium (16), the collimator (18) is placed over the holding tray (14) with the filter medium (16) positioned between the collimator (18) and the holding tray (14) and the samples disposed in the sample wells. The carrier plate (12), the holding tray (14), the filter medium (16), and the collimator (18) are provided with complementary keyed corners (22,34,19,46) to facilitate alignment of these elements relative to one another. The wells of the collimator (18) include respective lower rims (54) protruding into the filter medium (16) to minimize crosstalk through the filter medium (16). The cover film (20) seals the microplate assembly (10) so that the samples are prepared for analysis.

公开(公告)号：EP0571661A1

公开(公告)日：1993-12-01

申请号：EP92119631.7

申请日：1992-11-17

Applicant: Packard Instrument Company, Inc.

Inventor： Manns, Roy L. ,  Kolb, Alfred J. ,  Effertz, Bernard S.

IPC: B01L3/00 ,  G01N21/03

CPC classification number: B01L3/5085 ,  B29C45/16 ,  G01N21/03 ,  G01N2021/0346

Abstract: A microplate (10) forms a multiplicity of sample wells for holding samples to be assayed by light emissions or light transmission. The plate (10) comprises an upper plate (11) forming the side walls (13) of the sample wells, the side walls (13) being opaque so that light cannot be transmitted between adjacent wells through the side walls, (13) and a lower plate (12) forming the bottom walls (14) of the sample wells, the bottom walls (14) being transparent to allow the transmission of light therethrough. Bands of opaque material are provided within the lower plate (12) and surrounding each well to block the transmission of light between adjacent wells through the lower plate (12).

Abstract translation: 微板（10）形成多个样品孔，用于保持通过光发射或光透射测定的样品。 板（10）包括形成样品孔的侧壁（13）的上板（11），侧壁（13）是不透明的，使得光不能通过侧壁（13）和 形成样品井的底壁（14）的下板（12），底壁（14）是透明的，以允许光透过。 不透明材料的带设置在下板（12）内并且围绕每个井以阻挡相邻井之间通过下板（12）的透射。