公开(公告)号：EP2554654A4

公开(公告)日：2014-07-09

申请号：EP11762217

申请日：2011-03-28

Applicant: PANASONIC CORP

Inventor： YAMAMOTO TAKEKI ,  NAKATANI MASAYA ,  USHIO HIROSHI ,  YAMADA YOSHIKI ,  NAKANO YUSUKE

IPC: C12M1/34 ,  G01N27/08 ,  G01N27/28 ,  G01N27/30 ,  G01N27/416 ,  G01N33/487

CPC classification number: G01N33/48728 ,  G01N27/08

公开(公告)号：EP2650678A4

公开(公告)日：2014-03-05

申请号：EP12734301

申请日：2012-01-11

Applicant: PANASONIC CORP

Inventor： HASHIMOTODANI KIYOSHI ,  NAKANO YUSUKE ,  NAKATANI MASAYA ,  YAMAMOTO TAKEKI ,  YAMADA YOSHIKI ,  OKA TAKUYA ,  USHIO HIROSHI

IPC: G01N33/487 ,  G01N27/28 ,  G01N27/327

CPC classification number: G01N33/48728 ,  Y10T428/31663

公开(公告)号：JP2012141142A

公开(公告)日：2012-07-26

申请号：JP2010291935

申请日：2010-12-28

Applicant: Panasonic Corp ,  パナソニック株式会社

Inventor： YAMADA YOSHIKI ,  NAKATANI MASAYA

IPC: G01N33/543 ,  C07K17/00 ,  C12M1/00 ,  C12N11/00

Abstract: PROBLEM TO BE SOLVED: To provide a probe immobilization method in which probe modification is restricted.SOLUTION: A probe immobilization method for fixing a probe 14 onto a substrate 11 comprises the steps of: preparing a substrate 11 to which DNA 12 is coupled and a probe 14 to which DNA-coupled protein 13 is coupled; and fixing the probe 14 via the DNA 12 at the substrate 11. The DNA 12 is thus formed on the substrate 11, and the DNA 12 is coupled to a DNA-coupling domain, which is part of the DNA-coupled protein 13 coupled to the probe 14, allowing the probe 14 to be adsorbed and fixed to the substrate, thereby making it possible to restrict the probe 14 from being modified with change in pH and temperature in a chemical reaction.

Abstract translation: 要解决的问题：提供限制探针修饰的探针固定方法。 解决方案：用于将探针14固定到基底11上的探针固定方法包括以下步骤：制备与DNA 12偶联的底物11和与DNA偶联蛋白13偶联的探针14; 并且通过DNA 12在底物11上固定探针14.DNA 12因此形成在底物11上，并且DNA 12与DNA偶联结构域偶联，DNA偶联结构域是DNA偶联蛋白13的一部分， 探针14，允许探针14被吸附并固定到基底上，从而可以限制探针14在化学反应中pH和温度的变化而被改变。 版权所有（C）2012，JPO＆INPIT

公开(公告)号：JP2012141140A

公开(公告)日：2012-07-26

申请号：JP2010291932

申请日：2010-12-28

Applicant: Panasonic Corp ,  パナソニック株式会社

Inventor： YAMADA YOSHIKI ,  TANEMURA AKINA ,  NAKATANI MASAYA

IPC: G01N33/543 ,  G01N33/566

Abstract: PROBLEM TO BE SOLVED: To provide a probe immobilization method in which probe modification is restricted.SOLUTION: A probe immobilization method for immobilizing a probe 12 onto a substrate 11 comprises the steps of: preparing the substrate 11 and the probe 12; and processing the substrate 11 to fix the probe 12 onto the substrate 11 without chemical reaction. This allows the probe 12 to be adsorbed and fixed to the substrate 11 in a natural reaction without any chemical reaction upon fixing the probe 12, thereby making it possible to restrict the probe 12 from being modified with change in pH and temperature in a chemical reaction.

Abstract translation: 要解决的问题：提供限制探针修饰的探针固定方法。 解决方案：用于将探针12固定到基底11上的探针固定方法包括以下步骤：制备基底11和探针12; 并且处理基板11以将探针12固定在基板11上而没有化学反应。 这样就可以使探针12在固定探针12时以自然的反应被吸附固定在基板11上而没有任何化学反应，从而可以限制探针12在化学反应中的pH和温度的变化中被改变 。 版权所有（C）2012，JPO＆INPIT

公开(公告)号：JP2011085579A

公开(公告)日：2011-04-28

申请号：JP2010162500

申请日：2010-07-20

Applicant: Panasonic Corp ,  パナソニック株式会社

Inventor： USHIO KOJI ,  NAKATANI MASAYA ,  TAKAHASHI MAKOTO ,  YAMADA YOSHIKI ,  OKA TAKUYA ,  NAKANO YUSUKE ,  YAMAMOTO KENJU

IPC: G01B11/12 ,  G01B11/24 ,  G01N21/88

Abstract: PROBLEM TO BE SOLVED: To improve productivity of a cell electrophysiological sensor. SOLUTION: The method of manufacturing the cell electrophysiological sensor includes steps of: irradiating a sensor chip 16 having a through-hole 15 mounted on a mounting substrate 17 or the sensor chip 16 having the through-hole 15 before mounted on the mounting substrate 17 with light from a light source 11 to acquire optical information transmitted through the through-hole 15 of the sensor chip 16 by an optical information acquisition means 12; individually making the optical information acquired during the prior step into a brightness value in a minimum unit by an optical information processing means 13; and determining whether a shape of the through-hole 15 is good in the atmosphere by a structure pattern including the minimum unit of a standard value or larger of the brightness value obtained during the prior step. Thus, the shape of the through-hole 15 of the cell electrophysiological sensor 14 can be simply and quickly inspected. COPYRIGHT: (C)2011,JPO&INPIT

Abstract translation: 要解决的问题：提高细胞电生理传感器的生产力。 解决方案：制造细胞电生理传感器的方法包括以下步骤：照射具有安装在安装基板17上的通孔15的传感器芯片16或具有通孔15的传感器芯片16安装在安装件 基板17与来自光源11的光通过光信息获取装置12获取透过传感器芯片16的通孔15的光信息; 通过光学信息处理装置13将在前一步骤中获取的光学信息单独地制成最小单位的亮度值; 并且通过包括在前一步骤中获得的亮度值的标准值或更大的标准值的最小单位的结构图案来确定通孔15的形状是否良好。 因此，细胞电生理传感器14的通孔15的形状可以简单快速地进行检查。 版权所有（C）2011，JPO＆INPIT

公开(公告)号：JP2011002268A

公开(公告)日：2011-01-06

申请号：JP2009143851

申请日：2009-06-17

Applicant: Panasonic Corp ,  パナソニック株式会社

Inventor： USHIO KOJI ,  NAKATANI MASAYA ,  TAKAHASHI MAKOTO ,  YAMADA YOSHIKI ,  OKA TAKUYA ,  NAKANO YUSUKE ,  YAMAMOTO KENJU

IPC: G01N27/28 ,  C12M1/34 ,  G01N27/416

Abstract: PROBLEM TO BE SOLVED: To realize a cell electrophysiologic sensor of high measuring precision.SOLUTION: The cell electrophysiologic sensor is equipped with a mounting substrate 8 and the sensor chips 9 held to the lower end parts of the through-holes piercing the mounting substrate 8 from the upper surface to the undersurface thereof. Each of the sensor chips 9 has a continuity hole 12 for allowing the upper surface side of the mounting substrate 8 to communicate with the undersurface side thereof, and first and second grooves 19 and 20 are formed to the undersurface of the mounting substrate 8 at the contact part with the liquid flowing the lower part of the mounting substrate 8 so as not to be parallel to each other. By this constitution, the cell electrophysiologic sensor of high measuring precision can be realized.

Abstract translation: 要解决的问题：实现高测量精度的细胞电生理传感器。解决方案：细胞电生理传感器配备有安装基板8，并且传感器芯片9保持在贯穿安装基板8的通孔的下端部分 从上表面到下表面。 每个传感器芯片9具有用于允许安装基板8的上表面侧与其下表面连通的连续孔12，并且第一和第二凹槽19和20形成在安装基板8的下表面处 与流过安装基板8的下部的液体接触，以不彼此平行。 通过该结构，可以实现高测量精度的细胞电生理传感器。

公开(公告)号：JP2010208885A

公开(公告)日：2010-09-24

申请号：JP2009056199

申请日：2009-03-10

Applicant: Panasonic Corp ,  パナソニック株式会社

Inventor： YAMAMOTO KENJU ,  NAKATANI MASAYA ,  TAKAHASHI MAKOTO ,  YAMADA YOSHIKI

IPC: C01B33/02 ,  C23C14/12 ,  C23C14/48 ,  C30B29/62

Abstract: PROBLEM TO BE SOLVED: To provide a silicon structure having a large surface area and exhibiting magnetic characteristic which is used for recovering a target material in a bioscreening and uses silicon having high machinability, low melting point and low density. SOLUTION: The silicon structure 10 is provided with a base material 11 and a plurality of fibrous projecting materials 12 containing silicon dioxide as an essential component and directly joined to the surface of the base material 11 which contains silicon as an essential component. A magnetic body is supported by the fibrous projecting material 12. The surface area is increased by forming the plurality of the fibrous projecting materials 12 comprising silicon dioxide to be densely entangled and magnetic separation and highly efficient recovery are available by supporting the magnetic body 13 on the fibrous projecting materials 12. COPYRIGHT: (C)2010,JPO&INPIT

Abstract translation: 要解决的问题：提供具有大的表面积并具有磁特性的硅结构，其用于在生物筛选中回收目标材料并使用具有高切削性，低熔点和低密度的硅。 解决方案：硅结构10设置有基材11和多个含有二氧化硅作为主要成分并直接接合到基材11的表面的纤维状突出材料12，该基材11含有硅作为主要成分。 磁性体由纤维状突出材料12支撑。通过形成多个包含二氧化硅的纤维状突出材料12以使其密集地缠绕并进行磁性分离，从而提高表面积，通过将磁性体13支撑在磁性体13上 纤维状突出材料12.版权所有（C）2010，JPO＆INPIT

公开(公告)号：JP2014173969A

公开(公告)日：2014-09-22

申请号：JP2013046315

申请日：2013-03-08

Applicant: Panasonic Corp ,  パナソニック株式会社

Inventor： KUROTAKE HIROYUKI ,  NAKATANI MASAYA ,  USHIO KOJI ,  YAMADA YOSHIKI

IPC: G01N35/02 ,  C12M1/00

Abstract: PROBLEM TO BE SOLVED: To provide a multi-well plate with a high measurement accuracy.SOLUTION: A multi-well plate 100 is a plate 11 that has plural recesses 12 opened in the upper surface. At least the upper face of the plate 11 between a recess 12a and a neighboring recess 12b has the hydrophobic property higher than the bottom of the recesses 12. Therefore, a solution is prevented from mixing among the neighboring recesses. Thus the measurement accuracy is increased.

Abstract translation: 要解决的问题：提供具有高测量精度的多孔板。解决方案：多孔板100是具有在上表面中敞开的多个凹部12的板11。 至少在凹部12a和相邻的凹部12b之间的板11的上表面具有高于凹部12的底部的疏水性。因此，防止溶液在相邻的凹部之间混合。 因此，测量精度提高。

公开(公告)号：JP2012141141A

公开(公告)日：2012-07-26

申请号：JP2010291934

申请日：2010-12-28

Applicant: Panasonic Corp ,  パナソニック株式会社

Inventor： YAMADA YOSHIKI ,  TANEMURA AKINA ,  NAKATANI MASAYA ,  YAMAMOTO KENJU

IPC: G01N33/543 ,  C12M1/00

Abstract: PROBLEM TO BE SOLVED: To provide a probe immobilization method in which probe modification is restricted.SOLUTION: A probe immobilization method for fixing a probe 13 onto a substrate 11 comprises the steps of: preparing a substrate having a hydrophobic substrate surface and a probe having a hydrophobic portion at the end thereof; aligning the probe in parallel to an oil-water interface of a solution having the oil-water interface in at least part thereof; and dropping the solution to which the probe is aligned in parallel, onto the substrate having the hydrophobic substrate surface, to fix the probe onto the substrate. This allows the probe to be adsorbed and fixed to the substrate in a natural reaction without any chemical reaction upon fixing the probe, thereby making it possible to restrict the probe from being modified with change in pH and temperature in a chemical reaction.

Abstract translation: 要解决的问题：提供限制探针修饰的探针固定方法。 解决方案：用于将探针13固定到基底11上的探针固定方法包括以下步骤：制备具有疏水基底表面的基底和在其末端具有疏水部分的探针; 将探针与其至少一部分具有油 - 水界面的溶液的油 - 水界面平行地对准; 并将探针平行排列的溶液滴加到具有疏水性基材表面的基材上，将探针固定在基材上。 由此，能够在固定探针时，在自然反应中将探针吸附固定在基板上而没有任何化学反应，从而可以限制在化学反应中pH和温度变化的探针被修饰。 版权所有（C）2012，JPO＆INPIT