公开(公告)号：AU637734B2

公开(公告)日：1993-06-03

申请号：AU8882391

申请日：1991-12-05

Applicant: PHILLIPS PETROLEUM COMPANY

Inventor： RICHARD G. BUCKHOLZ

IPC: C12N1/19 ,  C12N9/16 ,  C12N15/09 ,  C12N15/55 ,  C12N15/81 ,  C12P21/02 ,  C12R1/84

Abstract: The present invention discloses the Pichia pastoris acid phosphatase gene, which includes the 5' regulatory region, signal sequence, structural gene, and 3' transcription termination sequence. Also disclosed are methods of using these fragments, which include but are not limited to the secretion of proteins from cells and the regulation of the transcription of DNA. DNA vectors containing the acid phosphatase gene or fragments thereof and hosts transformed with these vectors are also disclosed. Additionally, integrative vectors which direct integration at the Pichia pastoris PHO1 locus and a method of identifying these disruptants is disclosed.