公开(公告)号：US20250163408A1

公开(公告)日：2025-05-22

申请号：US18743720

申请日：2024-06-14

Applicant: POSTECH RESEARCH AND BUSINESS DEVELOPMENT FOUNDATION

Inventor： Jeong Wook LEE ,  Yun-nam CHOI ,  Dong Hyeon KIM ,  Seong Beom LEE

IPC: C12N15/11 ,  C12N1/20 ,  C12N9/00

Abstract: The present invention relates to a genetic biocontainment technology to prevent theft or misuse of biological resources (e.g., nucleic acid molecule, plasmid or microbial strain containing the nucleic acid molecule, or sequence information associated with the nucleic acid molecule) using a quadruplet codon and decoding system thereof. In particular, the present invention relates to a system or a microbial strain comprising a nucleic acid molecule in which a triplet codon encoding a specific amino acid in a nucleic acid sequence has been replaced with a quadruplet codon, wherein genetic biocontainment occurs in an environment where tRNA for the quadruplet codon does not exist, and a method using the same. The present invention also relates to a biocontainment method to protect sequence information using a quadruplet codon.