公开(公告)号：US10416068B2

公开(公告)日：2019-09-17

申请号：US14484248

申请日：2014-09-11

Applicant: Purdue Research Foundation

Inventor： Garth Jason Simpson ,  Ryan Douglas Muir ,  Shane Zachary Sullivan

IPC: H04R3/00 ,  G01N15/14

Abstract: A quantitative optical microscopy arrangement is described. Specifically, a digital filter derived from linear discriminant analysis is described for recovering impulse responses in applications that may include photon counting from a high speed photodetector and applied to remove ringing distortions from impedance mismatch in multiphoton fluorescence microscopy. Training of the digital filter is achieved by defining temporally coincident and non-coincident transients and identifying the projection within filter-space that best separates the two classes. The training allows rapid data analysis by digital filtering. The LDA filter is also capable of recovering deconvolved impulses for single photon counting from highly distorted ringing waveforms from an impedance mismatched photomultiplier tube. The LDA filter is also successful in removing these ringing distortions from two-photon excited fluorescence micrographs and may extend the dynamic range of photon counting by about three orders of magnitude through minimization of detector paralysis.

公开(公告)号：US20160070093A1

公开(公告)日：2016-03-10

申请号：US14484248

申请日：2014-09-11

Applicant: Purdue Research Foundation

Inventor： Garth Jason Simpson ,  Ryan Douglas Muir ,  Shane Zachary Sullivan

IPC: G02B21/36 ,  G01J1/44 ,  G02B21/06

CPC classification number: G01N15/1429

Abstract: A quantitative optical microscopy arrangement is described. Specifically, a digital filter derived from linear discriminant analysis is described for recovering impulse responses in applications that may include photon counting from a high speed photodetector and applied to remove ringing distortions from impedance mismatch in multiphoton fluorescence microscopy. Training of the digital filter is achieved by defining temporally coincident and non-coincident transients and identifying the projection within filter-space that best separates the two classes. The training allows rapid data analysis by digital filtering. The LDA filter is also capable of recovering deconvolved impulses for single photon counting from highly distorted ringing waveforms from an impedance mismatched photomultiplier tube. The LDA filter is also successful in removing these ringing distortions from two-photon excited fluorescence micrographs and may extend the dynamic range of photon counting by about three orders of magnitude through minimization of detector paralysis.

Abstract translation: 描述了定量光学显微镜装置。 具体地，描述了从线性判别分析得到的数字滤波器，用于在应用中恢复脉冲响应，所述应用可包括来自高速光电检测器的光子计数，并应用于从多光子荧光显微镜中的阻抗失配中消除振铃失真。 数字滤波器的训练通过定义时间上一致和非重合的瞬变来实现，并且识别过滤器空间内的最佳分离两个类别的投影。 该培训允许通过数字滤波进行快速数据分析。 LDA滤波器还能够从阻抗不匹配的光电倍增管的高度失真的振荡波形中回收单光子计数的去卷积脉冲。 LDA滤波器也可以成功地从双光子激发的荧光显微照片中去除这些振铃失真，并且可以通过最小化检测器麻痹来将光子计数的动态范围扩展大约三个数量级。