公开(公告)号：AU2447702A

公开(公告)日：2002-05-15

申请号：AU2447702

申请日：2001-11-02

Applicant: QIN DINYU ,  STEWART MATTHEW ,  STAHL DAVID A

Inventor： QIN DINYU ,  STEWART MATTHEW ,  STAHL DAVID A

IPC: H02K1/27 ,  H02K11/00 ,  H02K21/12 ,  H02K1/22

公开(公告)号：WO9918234A3

公开(公告)日：2000-09-28

申请号：PCT/EP9806286

申请日：1998-10-02

Applicant: SUEZ LYONNAISE DES EAUX ,  UNIV NORTHWESTERN ,  GUILLOT EMMANUELLE ,  URBAIN VINCENT ,  MANEM JACQUES ,  RITTMANN BRUCE E ,  STAHL DAVID A ,  FLAX JODI ,  WAGNER MICHAEL

Inventor： GUILLOT EMMANUELLE ,  URBAIN VINCENT ,  MANEM JACQUES ,  RITTMANN BRUCE E ,  STAHL DAVID A ,  FLAX JODI ,  WAGNER MICHAEL

IPC: C12N15/09 ,  C12Q1/68

CPC classification number: C12Q1/689

Abstract: This invention relates to means of qualitative and quantitative analysis of microbial populations potentially present in a sample. These means notably comprise the use of at least one RNA-targeted oligonucleotide probe for in situ hybridization in whole cells; followed by the extraction of those probes which have become hybridized by separation from their target and elution from the microbial cells; as well as the detection and measurement of said extracted probes.

Abstract translation: 本发明涉及定性和定量分析样品中潜在存在的微生物种群的方法。 这些意味着特别包括使用至少一种RNA靶向寡核苷酸探针在全细胞中进行原位杂交; 然后通过从其靶分离并从微生物细胞中洗脱而已经杂交的那些探针的提取; 以及所述提取的探针的检测和测量。