公开(公告)号：WO2015193348A1

公开(公告)日：2015-12-23

申请号：PCT/EP2015/063546

申请日：2015-06-17

Applicant: RHODIA OPERATIONS

Inventor： RAMAEN, Odile ,  SAUVEPLANE, Vincent ,  PANDJAITAN, Rudy ,  GELO-PUJIC, Mirjana

IPC: C12N1/32 ,  C12N15/52 ,  C12P7/24

CPC classification number: C12P7/24 ,  C12N1/32 ,  C12N15/52

Abstract: The present invention concerns a recombinant yeast comprising a set of exogenous nucleic acids, with each nucleic acid encoding a polypeptide involved in the biosynthesis pathway of a vanilloid, said set of exogenous nucleic acids encoding a set of polypeptides selected in a group comprising: • a set of enzymes allowing conversion of an aromatic amino acid into a vanilloid; and • a set of enzymes allowing conversion of a dehydroshikimic acid into a vanilloid; and wherein said recombinant yeast is devoid of a functional gene adh6 encoding for an alcohol dehydrogenase, and wherein said recombinant yeast has a reduced ability to decarboxylate phenylacrylic acids, which encompasses that said yeast does not comprise a fonctional fdcI gene and/ or a functional padl gene.

Abstract translation: 本发明涉及包含一组外源核酸的重组酵母，每种核酸编码参与香草素生物合成途径的多肽，所述一组外源核酸编码一组选自下组的多肽：·a 允许将芳族氨基酸转化成香草素的酶组; 和一组允许将脱水山梨酸转化为香草素的酶; 并且其中所述重组酵母缺乏编码醇脱氢酶的功能基因adh6，并且其中所述重组酵母具有降低苯基丙烯酸脱羧能力，其包括所述酵母不包含功能性fdcI基因和/或功能性片段 基因。

公开(公告)号：WO2015193371A1

公开(公告)日：2015-12-23

申请号：PCT/EP2015/063590

申请日：2015-06-17

Applicant: RHODIA OPERATIONS

Inventor： RAMAEN, Odile ,  PANDJAITAN, Rudy ,  GELO-PUJIC, Mirjana

IPC: C12N15/52 ,  C12P7/24

CPC classification number: C12P7/24 ,  C07C47/58 ,  C12N9/0008 ,  C12N9/1007 ,  C12N9/1288 ,  C12N9/88 ,  C12N15/52 ,  C12Y102/0103 ,  C12Y201/01068 ,  C12Y207/08007 ,  C12Y402/01118

Abstract: The present invention relates to methods for producing vanilloid compounds in a recombinant host, and in particular for converting a protocatechuic aldehyde into a substantially pure vanilloid. It further relates to novel yeast strains that are suitable for producing such vanilloid compounds.

Abstract translation: 本发明涉及在重组宿主中生产香草酸化合物的方法，特别是用于将原儿茶醛转化为基本上纯的香草酸。 它还涉及适用于生产这种香草酸化合物的新型酵母菌株。

公开(公告)号：EP3158069A1

公开(公告)日：2017-04-26

申请号：EP15728895.2

申请日：2015-06-17

Applicant: Rhodia Operations

Inventor： RAMAEN, Odile ,  PANDJAITAN, Rudy ,  GELO-PUJIC, Mirjana

IPC: C12N15/52 ,  C12P7/24

CPC classification number: C12P7/24 ,  C07C47/58 ,  C12N9/0008 ,  C12N9/1007 ,  C12N9/1288 ,  C12N9/88 ,  C12N15/52 ,  C12Y102/0103 ,  C12Y201/01068 ,  C12Y207/08007 ,  C12Y402/01118

Abstract: The present invention relates to methods for producing vanilloid compounds in a recombinant host, and in particular for converting a protocatechuic aldehyde into a substantially pure vanilloid. It further relates to novel yeast strains that are suitable for producing such vanilloid compounds.

Abstract translation: 本发明涉及用于在重组宿主中生产香草素化合物，以及特别是用于原儿茶醛转换成基本上纯香草素。 它进一步涉及新颖的酵母菌株做适于生产寻求香草素化合物。