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    POLYUBIQUITIN CDNA AND PRODUCTION OF LABELED UBIQUITIN
    2.
    发明专利
    POLYUBIQUITIN CDNA AND PRODUCTION OF LABELED UBIQUITIN 失效

    公开(公告)号：JPH09263A

    公开(公告)日：1997-01-07

    申请号：JP15461695

    申请日：1995-06-21

    Applicant: SAGAMI CHEM RES

    Inventor： KATO MASASHI ,  SAEKI MIHORO ,  KATO TAKAE ,  KIN NANJIYUN ,  SEKINE SHINGO

    IPC: C12N15/09 ,  C07H21/04 ,  C07K19/00 ,  C12N15/00 ,  C12P21/02 ,  C12Q1/68 ,  C12R1/91

    Abstract: PURPOSE: To obtain a new cDNA having a specific base sequence, coding the polyubiquitin, and used for producing a labeled ubiquitin useful as a reagent for diagnosing diseases causing by the dysbolism of ubiquitin and for researching the ubiquitin. CONSTITUTION: This polyubiquitin cDNA is a new human cDNA having a base sequence of the formula and coding the polyubiquitin and is useful for producing by a genetic recombination technique a labeled ubiquitin used as a reagent for diagnosing diseases causing by the dysbolism of the ubiquitin or for researching the ubiquitin, etc. The cDNA is obtained by determining the base sequence of a clone arbitrarily selected from the cDNA library of human lymphoma cell strain U937, retrieving an amino acid sequence coding the base sequence from a protein data base, screening a clone containing a cDNA coding the ubiquitin, recovering the gene and subsequently treating the recovered gene with a restriction enzyme. Thus, the cDNA is obtained as the cDNA of polyubiquitin where nine ubiquitin molecules are continuously connected to each other.

    GENE CODING FOR BIOSYNTHETIC ENZYME GROUP FOR EICOSAPENTAENOIC ACID AND PRODUCTION OF EICOSAPENTAENOIC ACID
    3.
    发明专利
    GENE CODING FOR BIOSYNTHETIC ENZYME GROUP FOR EICOSAPENTAENOIC ACID AND PRODUCTION OF EICOSAPENTAENOIC ACID 失效

    公开(公告)号：JPH08242867A

    公开(公告)日：1996-09-24

    申请号：JP348596

    申请日：1996-01-12

    Applicant: SAGAMI CHEM RES

    Inventor： YAZAWA KAZUYOSHI ,  YAMADA AKIKO ,  KONDO SEI ,  KATO MASASHI

    IPC: C12N15/09 ,  A61K31/20 ,  A61P7/02 ,  C07H21/04 ,  C12N1/21 ,  C12N9/00 ,  C12P7/64 ,  C12R1/19

    Abstract: PURPOSE: To obtain a new gene, having a specific base sequence, coding for a biosynthetic enzyme for eicosapentaenoic acid, having various physiological activities and useful for production, etc., of the biosynthetic enzyme for the eicosapentaenoic acid useful as a raw material, etc., for medicines, foods, feeds, etc. CONSTITUTION: Thus new gene codes for a biosynthetic enzyme for eicosapentaenoic acid with a base sequence containing a base sequence of the formula and producing the biosynthetic enzyme for the eicosapentaenoic acid, playing an important role as a constituent component of a biomembrane, having suppressing actions on blood platelet agglutination, lowering actions on neutral fats and cholesterol in blood, vasodepressor actions, etc., and useful as medicines, foods, feeds, etc., according to a genetic recombination method. The gene is obtained by culturing a microorganism, belonging to the Shewanella and having the ability to produce the eicosapentaenoic acid, then collecting the microbial cell, extracting a chromosomic DNA from the collected microbial cell, preparing a DNA library using the resultant chromosomic DNA and recovering the DNA from a clone having the productivity for the eicosapentaenoic acid.

    CLONING VECTOR PLASMID, VECTOR PRIMER DERIVED FROM THE SAME PLASMID AND PREPARATION OF CDNA BANK USING THE SAME PRIMER
    5.
    发明专利
    CLONING VECTOR PLASMID, VECTOR PRIMER DERIVED FROM THE SAME PLASMID AND PREPARATION OF CDNA BANK USING THE SAME PRIMER 失效

    公开(公告)号：JPH04117292A

    公开(公告)日：1992-04-17

    申请号：JP23833290

    申请日：1990-09-07

    Applicant: SAGAMI CHEM RES

    Inventor： KATO MASASHI ,  AOKI TAKASHI ,  UMEZAWA YURI

    IPC: C12N15/00 ,  C07K14/525 ,  C12N15/09 ,  C12N15/10 ,  C12N15/70 ,  C12N15/85

    Abstract: NEW MATERIAL:The title plasmid positioning a restriction enzyme site RE1 from promoter PR1 for mammalian cells toward downstream, promoter PR2 of RNA polymerase, restriction enzyme site RE2, restriction enzyme site RE3, restriction enzyme site RE4, restriction enzyme site RE5 and RNA polymerase promoter PR3, positioning restriction enzyme site RE6 at an arbitrary place of the restriction enzyme site RE1, replication origin OR1 for mammalian cells, replication origin OR2 of single strand phage, replication origin OR3 for Escherichia coil and a selecting marker at an arbitrary position. EXAMPLE:Vector pKA1. USE:Preparation of cDNA bank. PREPARATION:Plasmid pTZ18RP4 obtained from plasmid pTZ18R and plasmid pKAO obtained from plasmid pTZ19U are subjected to a process shown by the formula to give cloning vector pKA1 of example.

    HUMAN LAMININ B1 CHAIN POLYPEPTIDE FRAGMENT AND MANIFESTATION VECTOR TO PRODUCE SAME
    6.
    发明专利
    HUMAN LAMININ B1 CHAIN POLYPEPTIDE FRAGMENT AND MANIFESTATION VECTOR TO PRODUCE SAME 失效

    公开(公告)号：JPH02234679A

    公开(公告)日：1990-09-17

    申请号：JP5283589

    申请日：1989-03-07

    Applicant: SAGAMI CHEM RES

    Inventor： KATO MASASHI ,  MIWA KYOKO ,  OSADA HIROSHI ,  UMEZAWA YURI

    IPC: C12N15/09 ,  C07H21/04 ,  C07K1/20 ,  C07K14/00 ,  C12N1/21 ,  C12N15/12 ,  C12N15/70 ,  C12P21/02 ,  C12R1/19

    Abstract: PURPOSE:To obtain a human laminin B1 chain polypeptide fragment having cell bonding activity, obtainable in a large amount, expected to be useful as inhibitor against cancer metastasis, wound remedy, etc., containing a cell bond site of human laminin B1 chain. CONSTITUTION:A polypeptide fragment of human laminin B1 chain is produced by a manifestation vector capable of manifesting the polypeptide in a host bacterium, preferably Escherichia coli. Namely, Escherichia coli, especially strain of Escherichia coli free from properties of living in intestine, such as JM101 derived from Escherichia coli K-12 is transformed and the transformed strain is cultured to produce the polypeptide fragment of human laminin B1 chain in the transformed strain. The polypeptide produced in the cell of Escherichia coli is recovered and purified by conventional procedure to recover and purify the aimed human laminin B1 chain polypeptide fragment.

    EXPRESSION VECTOR
    8.
    发明专利
    EXPRESSION VECTOR 失效

    公开(公告)号：JPH0216982A

    公开(公告)日：1990-01-19

    申请号：JP16493588

    申请日：1988-07-04

    Applicant: SAGAMI CHEM RES ,  CENTRAL GLASS CO LTD ,  HODOGAYA CHEMICAL CO LTD ,  NIPPON SODA CO ,  NISSAN CHEMICAL IND LTD ,  TOSOH CORP

    Inventor： KATO MASASHI ,  MIWA KYOKO ,  UMEZAWA YURI

    IPC: C12N15/09 ,  C12N15/00 ,  C12N15/63

    Abstract: PURPOSE:To eliminate need of various enzymic treatments by forming an expression vector containing a leading frame, positioned on the downstream side of a polycloning site and containing three respective different termination codons. CONSTITUTION:An expression vector containing a starting codon, a polycloning site positioned on the downstream side thereof and a leading frame, positioned on the downstream side of the polycloning side and having three respective different termination codons is prepared. The above-mentioned polycloning site is at least three restriction enzyme sites, capable of producing smooth terminals and having wholly different recognition sequences thereof. Only one restriction enzyme site is present in each expression vector and the residual after dividing the number of bases from the starting point of the starting codon to the cleaved sections of the respective restriction enzyme sites is respectively 0, 1 or 2.

    PROTEIN AS CONSTITUENT OF HUMAN 26S PROTEASOME AND DNA ENCODING THE SAME
    9.
    发明专利
    PROTEIN AS CONSTITUENT OF HUMAN 26S PROTEASOME AND DNA ENCODING THE SAME 失效

    公开(公告)号：JPH1132774A

    公开(公告)日：1999-02-09

    申请号：JP20032497

    申请日：1997-07-25

    Applicant: SAGAMI CHEM RES

    Inventor： KATO MASASHI ,  SEKINE SHINGO

    IPC: C12N15/09 ,  C07K14/47 ,  C12N9/64 ,  C12N15/00 ,  C12R1/91

    Abstract: PROBLEM TO BE SOLVED: To obtain the subject new protein, composed of human 26S proteasome constituent protein having a specific amino acid sequence, capable of exhibiting an important role in an intracellular proteolysis system, and useful for, e.g. elucidation of proteasome functions, or diagnosis or treatment of malignant tumor or the like. SOLUTION: This protein is composed of human 26S proteasome constituent protein having a specific amino acid sequence shown by the formula, capable of exhibiting an important role in an intracellular proteolysis system, and useful for, e.g. elucidation of proteasome functions, or diagnosis or treatment of diseases, e.g. malign lump, in which proteasome is involved. The protein is obtained by (A) synthesizing cDNA using a mold of poly (A) RNA extracted from human cells, (B) preparing a library of cDNA derived from the human cells by the normal procedure using the above cDNA, and (C) genetically recombining a gene, obtained by screening using an oligonucleotide probe composed of partial, basic sequence of bovine 26S proteasome constituent protein, into a expression vector, and expressing it in a host cell, e.g. that of E coli.

    IDENTIFICATION OF GENE CODING TYPE II MEMBRANE PROTEIN
    10.
    发明专利
    IDENTIFICATION OF GENE CODING TYPE II MEMBRANE PROTEIN 失效

    公开(公告)号：JPH10243784A

    公开(公告)日：1998-09-14

    申请号：JP4777597

    申请日：1997-03-03

    Applicant: SAGAMI CHEM RES ,  PUROTEJIIN KK

    Inventor： KATO MASASHI ,  KOBAYASHI MIDORI

    IPC: C12N15/09 ,  C07H21/04 ,  C07K14/705 ,  C07K19/00 ,  C12N15/00 ,  C12P21/00 ,  C12Q1/68 ,  C12R1/91

    Abstract: PROBLEM TO BE SOLVED: To enable the identification of the gene coding type II membrane protein that is useful in development of medicines by expressing a fused gene between a specific DNA fragment and a gene coding a reporter protein in mammalian cells. SOLUTION: A DNA fragment to be identified (for example, HP10085 originating from the cDNA library of human lymphoma cell strain U937 resembling human initial activation antigen CD69) and the gene that codes reporter protein (for example, urokinase type plasminogen activator) are fused and the fused gene is recombined with pSSD1 vector and the recombinant vector is used to transform Escherichia coli JM109, from which plasmid DNA is extracted and transfected into mammalian cells (COS-7 cells). The judgment of whether or not the reporter protein is expressed on the membrane surface of the transfected culture cells is used as an indicator to enable the subject identification (by detecting the activity of the corresponding enzyme).

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