公开(公告)号：CA2462617A1

公开(公告)日：2003-05-15

申请号：CA2462617

申请日：2002-11-07

Applicant: SENSOR TECHNOLOGIES LLC

Inventor： DAVID WOLF E

IPC: G01N33/50 ,  C12M1/34 ,  C12Q1/68 ,  G01N21/78 ,  G01N33/15 ,  G01N33/53 ,  G01N33/542 ,  G01N33/543 ,  G01N33/566 ,  G01N33/68 ,  G01N33/58 ,  G01N21/64

Abstract: A method of identifying an analyte-ligand binding pair that exhibits non- radiative fluorescence resonance energy transfer (FRET) using a combinatoria l library. The method includes a) obtaining an analyte binding ligand from a combinatorial library that includes ligands, and b) attaching a label at lea st one of the analyte binding ligand and an analyte~analogue with at least one of a first component and a second component of a non-radiative fluorescence resonance energy transfer donor-acceptor pair (FRET pair) such that FRET occurs when the analyte-analogue is bound to the analyte binding ligand, and a change in FRET occurs when the analyte-analogue is not bound to the analyte binding ligand. The method also includes contacting a combinatorial library of ligands, which are optionally labeled with a component of a FRET pair, with analyte-analogue, which is optionally labeled with a component of a FRET pai r, and detecting the presence of FRET.

公开(公告)号：AU2002363530A1

公开(公告)日：2003-05-19

申请号：AU2002363530

申请日：2002-11-07

Applicant: SENSOR TECHNOLOGIES LLC

Inventor： DAVID WOLF E

IPC: G01N33/50 ,  C12M1/34 ,  C12Q1/68 ,  G01N21/78 ,  G01N33/15 ,  G01N33/53 ,  G01N33/542 ,  G01N33/543 ,  G01N33/566 ,  G01N33/68

公开(公告)号：EP1461617A4

公开(公告)日：2005-09-14

申请号：EP02802889

申请日：2002-11-07

Applicant: SENSOR TECHNOLOGIES LLC

Inventor： DAVID WOLF E

IPC: G01N33/50 ,  C12M1/34 ,  C12Q1/68 ,  G01N21/78 ,  G01N33/15 ,  G01N33/53 ,  G01N33/542 ,  G01N33/543 ,  G01N33/566 ,  G01N33/68

CPC classification number: G01N33/542

Abstract: A method of identifying an analyte-ligand binding pair that exhibits non-radiative fluorescence resonance energy transfer FRET using a combinatorial library. The method includes a obtaining an analyte binding ligand from a combinatorial library that includes ligands, and b attaching a label at least one of the analyte binding ligand and an analyte­analogue with at least one of a first component and a second component of a non-radiative fluorescence resonance energy transfer donor-acceptor pair FRET pair such that FRET occurs when the analyte-analogue is bound to the analyte binding ligand, and a change in FRET occurs when the analyte-analogue is not bound to the analyte binding ligand. The method also includes contacting a combinatorial library of ligands, which are optionally labeled with a component of a FRET pair, with analyte-analogue, which is optionally labeled with a component of a FRET pair, and detecting the presence of FRET.