公开(公告)号：US20240328945A1

公开(公告)日：2024-10-03

申请号：US18741387

申请日：2024-06-12

Applicant: SHENZHEN INSTITUTES OF ADVANCED TECHNOLOGY

Inventor： Leo E. WONG ,  Shuwen CHANG

IPC: G01N21/64 ,  C12N13/00

CPC classification number: G01N21/6428 ,  C12N13/00 ,  G01N2021/6439

Abstract: Disclosed are a detection method for protein liquid-liquid phase separation (LLPS) and use thereof. The method comprises: constructing a first expression vector for expressing an energy donor fluorescent molecule fused to a target protein and a second expression vector for expressing an energy acceptor fluorescent molecule fused to the target protein; transferring both expression vectors into primary cultured cells; stimulating the primary cultured cells while collecting fluorescence microscopic imaging data by using an appropriate fluorescence microscope; calculating a change in Förster resonance energy transfer (FRET) efficiency as a proxy for monitoring the process of LLPS and phase transition of the target protein.