公开(公告)号：WO2017144619A1

公开(公告)日：2017-08-31

申请号：PCT/EP2017/054244

申请日：2017-02-23

Applicant: SINGLE TECHNOLOGIES AB

Inventor： STUBBE, Raoul ,  SAHLGREN, Bengt ,  STRÖMQUIST, Johan

IPC: G01N21/64 ,  G01N21/47 ,  G02B21/00 ,  G02B21/33 ,  G02B21/26

CPC classification number: G01N21/6458 ,  G01N21/474 ,  G01N21/6486 ,  G01N2021/6419 ,  G01N2021/6471 ,  G01N2021/6478 ,  G01N2021/6482 ,  G01N2021/6484 ,  G01N2201/0833 ,  G01N2201/103 ,  G02B19/0019 ,  G02B19/0057 ,  G02B21/0032 ,  G02B21/0036 ,  G02B21/0064 ,  G02B21/0076 ,  G02B21/26 ,  G02B21/33 ,  G02B27/1006 ,  G02B27/141

Abstract: Apparatus for imaging a sample (109), said apparatus comprising: illumination means (101, 102, 103, 104, 105, 106, 107, 108) for illuminating said sample (109) simultaneously in a line focus or an array of foci; and detection means (108, 107, 106b, 111, 112, 113) for detecting photons emitted or scattered from a sample (109) simultaneously in an array of fields of view; wherein an array of sub-observation volumes in a sample (109), from which photons are emitted or scattered during imaging, is defined by the volumes in space where the line focus or array of foci from the illumination means (101, 102, 103, 104, 105, 106, 107, 108) overlap with the corresponding array of field of views of the detection means (108, 107, 106b, 111, 112, 113); a sample holder (110, 110a), preferably a cylindrical sample holder (110, 110a), configured to hold the sample (109) at a surface thereof, said sample holder (110, 110a) being rotatably arranged such that at least a portion of said sample (109) can be transported through at least one of said sub-observation volumes by rotating the sample holder (110, 110a).

Abstract translation: （109）成像的设备，所述设备包括：照明装置（101,102,103,104,105,106,107,108），用于同时照亮所述样品（109）和所述样品 线焦点或焦点阵列; 和检测装置（108,107,106b，111,112,113），用于同时在一个视场阵列中检测从样本（109）发射或散射的光子; 其中在成像过程中从其发射或散射光子的样本（109）中的子观察体积的阵列由空间体积限定，其中来自照明装置（101,102,103）的线焦点或焦点阵列 ，104,105,106,107,108）与所述检测装置（108,107,106b，111,112,113）的所述视场的对应阵列重叠。 样品保持器（110,110a），优选圆柱形样品保持器（110,110a），被配置为将样品（109）保持在其表面处，所述样品保持器（110,110a）可旋转地布置成使得至少一部分 可以通过旋转样本保持器（110,110a）将所述样本（109）的至少一个传送通过所述子观察容积中的至少一个。