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1.发明公开Improved process for conducting lipase enzyme hydrolysis 失效Verfahren zurDurchführungvon enzymematischen Hydrolysen mit Lipasen。
公开(公告)号：EP0257716A2
公开(公告)日：1988-03-02
申请号：EP87201590.4
申请日：1987-08-21
Applicant: STAUFFER CHEMICAL COMPANY
Inventor： Dahod, Samun Kalim
CPC classification number: C12P7/40 , C12P1/00 , C12P41/005
Abstract: Lipase hydrolysis of an ester group of a substrate can be conducted in the absence of large quantities of water and excess enzyme without extensive inhibition of enzyme activity due to water-soluble product alcohol. A lipase reaction can be efficiently conducted by dispersing a water-immiscible organic substrate phase in an aqueous phase, washing the reaction continuously while the reaction mixture is maintained in the dispersed state, and continuously removing an amount of aqueous phase substantially equivalent to the washing solution added. The washing solution can be an aqueous neutralizing solution. By this process, water-soluble product alcohol can be removed from the reaction mixture without removal of substantial quantities of water-soluble enzyme.
Abstract translation: 在不存在大量水和过量酶的情况下，可以进行基质酯基的脂肪酶水解，而不会由于水溶性产物醇而对酶活性的广泛抑制。 通过将水不混溶的有机底物相分散在水相中，可以有效地进行脂肪酶反应，在反应混合物保持分散状态的同时连续洗涤反应，连续除去与洗涤液基本相当的水相量 添加。 洗涤溶液可以是水性中和溶液。 通过该方法，可以从反应混合物中除去水溶性产物醇，而不除去大量的水溶性酶。
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公开(公告)号：EP0257716A3
公开(公告)日：1989-11-08
申请号：EP87201590.4
申请日：1987-08-21
Applicant: STAUFFER CHEMICAL COMPANY
Inventor： Dahod, Samun Kalim
CPC classification number: C12P7/40 , C12P1/00 , C12P41/005
Abstract: Lipase hydrolysis of an ester group of a substrate can be conducted in the absence of large quantities of water and excess enzyme without extensive inhibition of enzyme activity due to water-soluble product alcohol. A lipase reaction can be efficiently conducted by dispersing a water-immiscible organic substrate phase in an aqueous phase, washing the reaction continuously while the reaction mixture is maintained in the dispersed state, and continuously removing an amount of aqueous phase substantially equivalent to the washing solution added. The washing solution can be an aqueous neutralizing solution. By this process, water-soluble product alcohol can be removed from the reaction mixture without removal of substantial quantities of water-soluble enzyme.
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