公开(公告)号：EP0935672B1

公开(公告)日：2008-12-17

申请号：EP97910799.2

申请日：1997-10-03

Applicant: STC.UNM

Inventor： SARTO, Gloria, E. ,  THOMPSON, Donald, M.

IPC: C12Q1/68 ,  G02B21/34

CPC classification number: G02B21/34 ,  C12Q1/6841 ,  C12Q2547/101 ,  C12Q2537/101 ,  C12Q2565/501

Abstract: A method, as shown in the figure, of preparing fluorescent in situ hybridization slides, comprising: providing a slide with a raised ring thereon; providing to the slide a DNA probe; providing to the slide cells and target DNA; providing to the slide a formamide-free mixture of 10 % +/- 2 % by weight dextran sulfate, and 15 %-25 % glycerol and 0.9 % by weight salt or a solution of 10 % +/- 2 % by weight dextran sulfate, 10-30 % by volume formamide and 0.9 % by weight salt; placing a coverglass on the ring; denaturing and hybridizing the slide; and performing a post-hybridization wash of the slide.