公开(公告)号：AU2005318369B2

公开(公告)日：2012-02-09

申请号：AU2005318369

申请日：2005-12-23

Applicant: STICHTING KATHOLIEKE UNIVERSITEIT

Inventor： VERHAEGH GERALD ,  WITJES ALFRED J ,  SCHALKEN JACK A ,  HESSELS DAPHNE

IPC: C12Q1/68

Abstract: Described herein are methods and kits for prognosis of prostate cancer in a subject. The methods comprises: (a) determining the ratio of PCA3 and PSA expression in a urine sample and (b) correlating the value of the PCA3/PSA ratio with the aggressiveness and mortality risk of prostate cancer in the subject. Kits for prognosing prostate cancer are also described. More particularly, the present invention features a method for prognosing prostate cancer in a biological sample of a patient comprising: assessing the amount of a prostate cancer specific PCA3 mRNA and the amount of PSA in the biological sample; determining a ratio value of this amount of prostate cancer specific PCA3 mRNA over the amount of PSA; comparing the ratio value to at least one predetermined cut-off value, wherein a ratio value above the predetermined cut-off value is indicative of a higher risk of mortality of prostate cancer as compared to a ratio value below the predetermined cut-off value.

公开(公告)号：AU2004254043A1

公开(公告)日：2005-01-13

申请号：AU2004254043

申请日：2004-06-30

Applicant: STICHTING KATHOLIEKE UNIVERSITEIT UNIVERSITY MEDICAL CENTRE NIJMEGEN

Inventor： VERHAEGH GERALD ,  SMIT FRANK ,  SCHALKEN JACK A ,  HESSELS DAPHNE

IPC: C12Q1/68

Abstract: The invention relates to a method to diagnose prostate cancer by detecting a PCA3 sequence. In one embodiment the method and kit enables amplification of PCA3 RNA through an exon-exon junction of a spliced PCA3 mRNA, and methods and kits to detect an amplified PCA3 RNA, using a probe which spans the amplified exon-exon junction. The methods and kits can detect a PCA3 RNA lacking one intron or more. Also provided are methods of detecting PCA3 RNA expressed in non-prostate tissue or cells of the urinary tract, which comprises PCA3 intron 3. In addition, methods are provided to determine whether a sample from a subject contains or lacks prostate cells, by performing a hybridization and/or amplification reaction on RNA from the sample to detect the presence or level of PCA3 RNA that lacks at least one intron and distinguishing a prostate cell from a non-prostate cell.

公开(公告)号：AU2010201771B2

公开(公告)日：2013-01-10

申请号：AU2010201771

申请日：2010-05-04

Applicant: STICHTING KATHOLIEKE UNIVERSITEIT THE UNIVERSITY MEDICAL CENTRE NIJMEGEN

Inventor： SCHALKEN JACK A ,  VERHAEGH GERALD ,  HESSELS DAPHNE ,  SMIT FRANK

IPC: C12Q1/68

Abstract: The present invention relates, in general, to the diagnosis of prostate cancer via the detection of a prostate cancer marker. More specifically, the present invention relates to a method of diagnosing prostate cancer in a patient by detecting the PCA3 sequence, and more particularly a PCA3 RNA sequence. The invention also relates to kits containing nucleic acid primers and/or kits containing nucleic acid primers and probes to diagnose, assess, or prognose a human afflicted with prostate cancer.