公开(公告)号：US20170356036A1

公开(公告)日：2017-12-14

申请号：US15488819

申请日：2017-04-17

Applicant: STOKES BIO LIMITED

Inventor： Mark B. DAVIES ,  Tara DALTON

IPC: C12Q1/68 ,  B01L3/00 ,  B01L7/00

CPC classification number: C12Q1/6851 ,  B01L3/502784 ,  B01L7/525 ,  B01L2300/0838 ,  B01L2300/0867 ,  B01L2400/0487 ,  C12Q2561/101 ,  C12Q2563/159 ,  C12Q2565/629

Abstract: Methods of conducting a nucleic acid reaction, including methods for performing digital PCR using a “droplet-in-oil” technology, may include at least partially segmenting a starting sampled into a set of sample droplets each containing on average about one or fewer copies of a target nucleic acid. The droplets are passed in a continuous flow of immiscible carrier fluid through a channel that passes through a thermal cycler, whereby the target is amplified. In one implementation, the droplets are about 350 nl each and the number of positively amplified droplets is counted at the near-saturation point.