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公开(公告)号：US20240141421A1
公开(公告)日：2024-05-02
申请号：US18384971
申请日：2023-10-30
Applicant: Stokes Bio Limited
Inventor： Mark DAVIES , Tara DALTON
CPC classification number: C12Q1/686 , B01F25/10 , B01F33/30 , B01L3/50273 , B01L3/502776 , B01L3/502784 , B01L7/525 , G01N35/08 , B01L2200/0636 , B01L2200/0673 , B01L2300/087 , B01L2400/0409 , B01L2400/0487 , G01N35/1095
Abstract: A microfluidic analysis system (1) performs polymerase chain reaction (PCR) analysis on a bio sample. In a centrifuge (6) the sample is separated into DNA and RNA constituents. The vortex is created by opposing flow of a silicon oil primary carrier fluid effecting circulation by viscous drag. The bio sample exits the centrifuge enveloped in the primary carrier fluid. This is pumped by a flow controller (7) to a thermal stage (9). The thermal stage (9) has a number of microfluidic devices (70) each having thermal zones (71, 72, 73) in which the bio sample is heated or cooled by heat conduction to/from a thermal carrier fluid and the primary carrier fluid. Thus, the carrier fluids envelope the sample, control its flowrate, and control its temperature without need for moving parts at the micro scale.
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公开(公告)号：US20170216846A1
公开(公告)日：2017-08-03
申请号：US15429765
申请日：2017-02-10
Applicant: STOKES BIO LIMITED
Inventor： Mark DAVIES , Tara DALTON
CPC classification number: B01L7/525 , B01L3/5027 , B01L3/502715 , B01L3/502784 , B01L2200/0673 , B01L2300/0627 , B01L2300/0654 , B01L2300/0838 , B01L2300/0867 , B01L2300/18 , B01L2300/1822 , B01L2300/185 , B01L2400/0487 , C12Q1/686 , G01N21/6428 , G01N2201/0826 , G01N2201/0833
Abstract: A thermal cycling device comprising a number of fixed thermal zones and a fixed conduit passing through the thermal zones. A controller maintains each thermal zone including its section of conduit at a constant temperature. A series of droplets flows through the conduit so that each droplet is thermally cycled, and a detection system detects fluorescence from droplets at all of the thermal cycles. The conduit is in a single plane, and so a number of thermal cycling devices may be arranged together to achieve parallelism. The flow conduit comprises a channel and a capillary tube inserted into the channel. The detection system may perform scans along a direction to detect radiation from a plurality of cycles in a pass.
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公开(公告)号：US20210299619A1
公开(公告)日：2021-09-30
申请号：US17197177
申请日：2021-03-10
Applicant: STOKES BIO LIMITED
Inventor： Mark DAVIES , Tara DALTON
Abstract: A system for analyzing a biological sample may include at least one sample acquisition stage comprising a sample acquisition device for acquiring the biological sample from a sample source; a droplet generator device for forming a droplet wrapped in an immiscible carrier fluid, wherein the wrapped droplet comprises at least the biological sample and a reagent, the droplet generator configured to receive the biological sample transferred from the sample acquisition device; a collection vessel for collecting the wrapped sample droplet from the droplet generator, the vessel configured to contain a carrier fluid for receiving and protecting the sample droplet; and an analysis system for analyzing the wrapped sample droplet and detecting products of a polymerase chain reaction.
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公开(公告)号：US20140045185A1
公开(公告)日：2014-02-13
申请号：US13963253
申请日：2013-08-09
Applicant: Stokes Bio Limited
Inventor： Mark DAVIES , Tara DALTON
IPC: C12Q1/68
CPC classification number: C12Q1/686 , C12Q1/6809 , C12Q1/6886 , C12Q2545/101
Abstract: Measurement of gene expression relative to an endogenous control gene is prone to excessive variability between samples and even replicates. The disclosure provides methods for normalizing expression levels of a gene by scaling gene expression levels to that of the most highly expressed gene in the set of genes whose expression levels are measured, rather than a house-keeping gene.
Abstract translation: 相对于内源性对照基因的基因表达的测量在样品之间容易发生过度的变异，甚至复制。 本公开提供了通过将基因表达水平与表达水平测定的基因组中最高表达基因的基因表达水平相比较而不是保存基因来使基因表达水平正常化的方法。
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