公开(公告)号：CA2917912C

公开(公告)日：2019-09-17

申请号：CA2917912

申请日：2014-07-22

Applicant: STRECK INC

Inventor： FERNANDO M ROHAN ,  RYAN WAYNE L ,  HUNSLEY BRAD

IPC: A01N1/02 ,  G01N33/574

Abstract: Compositions and Methods for Stabilizing Circulating Tumor Cells Methods and compositions for stabilizing a biological sample for analysis, comprising the steps of obtaining in a sample collection device a biological sample from a subject, especially blood, the biological sample including at least one circulating tumor cell from the subject. The methods may include a step of contacting the biological sample with a protective agent composition that includes a preservative agent, an optional anticoagulant, and a quenching agent to form a mixture that includes the protective agent composition and the sample.

公开(公告)号：ES2649572T3

公开(公告)日：2018-01-12

申请号：ES10704474

申请日：2010-02-11

Applicant: STRECK INC

Inventor： FERNANDO M ROHAN

IPC: C12Q1/68 ,  G01N1/30 ,  G01N33/50

Abstract: Un procedimiento de estabilización de ARN fuera de las células dentro de una muestra de sangre extraída para el análisis que comprende extraer la muestra de sangre en un tubo de recogida de sangre que comprende: a. de 0,5 % a 20 % en peso de un inhibidor de nucleasa seleccionado de uno o más de pirocarbonato de dietilo, etanol, ácido aurintricarboxílico (ATA), formamida, complejos de vanadil-ribonucleósidos, macaloides, ácido etilendiaminotetraacético (EDTA), proteinasa K, heparina, hidroxilamina-oxígeno-ion cúprico, bentonita, sulfato de amonio, ditiotreitol (DTT), beta-mercaptoetanol, cisteína, ditioeritritol, y clorhidrato de tris(2-carboxietil)fosfina; b. de 20 % a 30 % en peso de un agente conservante, en el que el agente conservante se selecciona de diazolidinil urea y/o imidazolidinil urea.

公开(公告)号：CA2938275C

公开(公告)日：2021-08-31

申请号：CA2938275

申请日：2010-01-21

Applicant: STRECK INC

Inventor： FERNANDO M ROHAN ,  CHAO-WEI CHEN KATE

IPC: C12N15/10 ,  G01N33/48

Abstract: Compositions for preserving and processing fetal nucleic acids located within maternal blood samples are disclosed, wherein a sample of maternal blood containing fetal nucleic acids is treated with a composition to reduce both cell lysis of the maternal blood cells and deoxyribonuclease (DNase) and ribonuclease (RNase) activity within the fetal nucleic acids. Treatment of the sample with a composition as disclosed herein aids in increasing the arnount of fetal nucleic acids that can be identified and tested while maintaining the structure and integrity of the fetal nucleic acids.

公开(公告)号：DK2228453T3

公开(公告)日：2017-02-27

申请号：DK10000518

申请日：2010-01-20

Applicant: STRECK INC

Inventor： FERNANDO M ROHAN ,  CHAO-WEI CHEN KATE

IPC: C12Q1/68

公开(公告)号：CA2780536A1

公开(公告)日：2011-05-12

申请号：CA2780536

申请日：2010-11-08

Applicant: STRECK INC

Inventor： RYAN WAYNE L ,  FERNANDO M ROHAN

IPC: C12Q1/68 ,  C12N15/10

Abstract: A method for preserving and processing nucleic acids located within a blood sample is disclosed, wherein a blood sample containing nucleic acids is treated to reduce both blood cell lysis and nuclease activity within the blood sample. The treatment of the sample aids in increasing the integrity and amount of cellular nucleic acids that can be identified and tested while avoiding contamination of the isolated nucleic acids with cell -free nucleic acids. The preferred compositions for preserving and processing contain diazolidinyl urea (DU), EDTA, aurintricarboxylic acid (ATA), glyceraldehyde and sodium floride.

公开(公告)号：WO2013123030A3

公开(公告)日：2013-10-31

申请号：PCT/US2013025912

申请日：2013-02-13

Applicant: STRECK INC

Inventor： RYAN WAYNE L ,  FERNANDO M ROHAN ,  DAS KAUSIK

IPC: C12Q1/68

CPC classification number: C12N15/1003 ,  C12Q1/6806 ,  C12Q2527/125

Abstract: Methods and devices for stabilizing and preserving circulating cell- free plasma DNA for analysis are disclosed. The methods and devices use diazolidinyl urea or imidazolidinyl urea as a preservative, EDTA as an anticoagulant and glycine as a quenching agent to quench any free formaldehyde from the protective composition.

Abstract translation: 公开了用于稳定和保存循环的无细胞血浆DNA进行分析的方法和装置。 方法和装置使用重氮烷基脲或咪唑烷基脲作为防腐剂，EDTA作为抗凝剂，甘氨酸作为猝灭剂，从保护组合物中淬灭任何游离甲醛。

公开(公告)号：SI2814981T1

公开(公告)日：2017-11-30

申请号：SI201330792

申请日：2013-02-13

Applicant: STRECK INC

Inventor： RYAN WAYNE L ,  FERNANDO M ROHAN ,  DAS KAUSIK

IPC: C12Q1/00

公开(公告)号：ES2617147T3

公开(公告)日：2017-06-15

申请号：ES10000518

申请日：2010-01-20

Applicant: STRECK INC

Inventor： FERNANDO M ROHAN ,  CHAO-WEI CHEN KATE

IPC: C12Q1/68

Abstract: Un procedimiento exploratorio no invasivo prenatal para la identificación de características fetales, que comprende las etapas de: poner en contacto una muestra de sangre materna extraída, que incluye una pluralidad de células sanguíneas, con un agente protector de ácido nucleico, para evitar que las células sanguíneas (i) liberen ácidos nucleicos genómicos a la muestra de sangre y (ii) experimenten una actividad nucleasa que degrade los ácidos nucleicos fetales; aislar los ácidos nucleicos fetales de la muestra de sangre materna; y analizar los ácidos nucleicos fetales aislados para identificar una característica fetal en el que el agente protector incluye glicina e imidazolidinil urea, y ácido etilendiaminotetraacético en el que el agente protector contiene menos del 1 % en peso de formaldehído.

公开(公告)号：CA2917912A1

公开(公告)日：2015-01-29

申请号：CA2917912

申请日：2014-07-22

Applicant: STRECK INC

Inventor： FERNANDO M ROHAN ,  RYAN WAYNE L ,  HUNSLEY BRAD

IPC: A01N1/02 ,  G01N33/574

Abstract: Compositions and Methods for Stabilizing Circulating Tumor Cells Methods and compositions for stabilizing a biological sample for analysis, comprising the steps of obtaining in a sample collection device a biological sample from a subject, especially blood, the biological sample including at least one circulating tumor cell from the subject. The methods may include a step of contacting the biological sample with a protective agent composition that includes a preservative agent, an optional anticoagulant, and a quenching agent to form a mixture that includes the protective agent composition and the sample.

公开(公告)号：CA2690651A1

公开(公告)日：2011-01-22

申请号：CA2690651

申请日：2010-01-21

Applicant: STRECK INC

Inventor： FERNANDO M ROHAN ,  CHAO-WEI CHEN KATE

IPC: C12N15/10 ,  C12Q1/68

Abstract: A method for preserving and processing fetal nucleic acids located within maternal plasma is disclosed, wherein a sample of maternal blood containing fetal nucleic acids is treated to reduce both cell lysis of the maternal blood cells and deoxyribonuclease (DNase) and ribonuclease (RNase) activity within the fetal nucleic acids. The treatment of the sample aids in increasing the amount of fetal nucleic acids that can be identified and tested while maintaining the structure and integrity of the fetal nucleic acids.