公开(公告)号：AU2002246978A1

公开(公告)日：2002-07-24

申请号：AU2002246978

申请日：2002-01-10

Applicant: SYMYX TECHNOLOGIES INC

Inventor： CHARMOT DOMINIQUE ,  KLAERNER GERRIT ,  PIOTTI MARCELO E ,  BENOIT DIDIER ,  NOMULA SRINIVAS ,  MAZZOLA LAURA T

IPC: C08F293/00 ,  G01N33/543 ,  C08F220/00 ,  G01N33/545 ,  C08J7/16

Abstract: Sensors for determining the presence and concentration of biomolecules in a biological sample are provided in the form of polymer brushes, which comprise a substrate having a surface modified with a hydrophobic polymer segment, attached to which is a water-dispersible or water-soluble polymer segment having functional groups that bind probes. The method of synthesis of such sensors preferably includes use of controlled free radical polymerization techniques, which allows for controlled architecture polymers to modify the surface of the substrate, and the use of monomers possessing functional groups which do not require activation prior to probe attachment. In this manner functional groups in the polymer chain are removed from the surface, which allows for solution chemistry to be more realistically reproduced with the benefits of a solid bound probe.

公开(公告)号：WO02056021A3

公开(公告)日：2003-09-18

申请号：PCT/US0200746

申请日：2002-01-10

Applicant: SYMYX TECHNOLOGIES INC ,  KLAERNER GERRIT ,  BENOIT DIDIER ,  CHARMOT DOMINIQUE ,  NOMULA SRINIVAS ,  PIOTTI MARCELO E ,  MAZZOLA LAURA T

Inventor： KLAERNER GERRIT ,  BENOIT DIDIER ,  CHARMOT DOMINIQUE ,  NOMULA SRINIVAS ,  PIOTTI MARCELO E ,  MAZZOLA LAURA T

IPC: C08F293/00 ,  G01N33/543 ,  C08F220/00 ,  C08J7/16 ,  G01N33/545

CPC classification number: G01N33/54353 ,  C08F293/005

Abstract: Sensors for determining the presence and concentration of bio-molecules in a biological sample are provided in the form of polymer brushes, which comprise a substrate having a surface modified with a hydrophobic polymer segment, attached to which is a water-dispersible or water-soluble polymer segment having functional groups that bind probes. The method of synthesis of such sensors preferably includes use of controlled free radical polymerization techniques, which allows for controlled architecture polymers to modify the surface of the substrate, and the use of monomers possessing functional groups which do not require activation prior to probe attachment. In this manner functional groups in the polymer chain are removed from the surface, which allows for solution chemistry to be more realistically reproduced with the benefits of a solid bound probe.

Abstract translation: 用于确定生物样品中生物分子的存在和浓度的传感器以聚合物刷的形式提供，所述聚合物刷包含具有用疏水性聚合物链段修饰的表面的基底，其附着在水分散性或水溶性 具有结合探针的官能团的聚合物链段。 合成这种传感器的方法优选包括使用受控的自由基聚合技术，其允许可控结构聚合物改性底物的表面，以及使用具有在探针附着之前不需要活化的官能团的单体。 以这种方式，从表面去除聚合物链中的官能团，这允许溶液化学更具体地再现，具有固体结合探针的优点。