公开(公告)号：US20080182310A1

公开(公告)日：2008-07-31

申请号：US12059250

申请日：2008-03-31

Applicant: Su-hyeon KIM ,  Jeong-gun LEE

Inventor： Su-hyeon KIM ,  Jeong-gun LEE

IPC: C12N13/00

CPC classification number: C12M47/06 ,  B01L3/5027 ,  C12N1/066 ,  C12N13/00 ,  G01N1/44 ,  Y10T137/2496 ,  Y10T436/25

Abstract: A cell lysis device for lysing cells or viruses, comprising a cell lysis tube having a sample inlet; a pump connected to the cell lysis tube for transferring a sample into the tube; a sealing unit for reversibly sealing a specific region of the tube; and a laser source for generating a laser is provide. Further, a method of lysing cells or viruses using the cell lysis device is provide. The method comprises introducing a sample containing cells or viruses and optionally magnetic beads to the cell lysis tube through the sample inlet; transferring the sample to a specific region in the cell lysis tube by means of the pump; temporarily sealing the region of the cell lysis tube where the sample is placed with the sealing unit; irradiating the sample with the laser; removing the sealing unit from the cell lysis tube; and discharging the sample from the cell lysis tube by means of the pump.

公开(公告)号：US20090099782A1

公开(公告)日：2009-04-16

申请号：US12128680

申请日：2008-05-29

Applicant: Su-hyeon KIM ,  Yoon-kyoung CHO ,  Jeong-gun LEE

Inventor： Su-hyeon KIM ,  Yoon-kyoung CHO ,  Jeong-gun LEE

IPC: G01N33/50

CPC classification number: G01N35/00732 ,  G01N35/00069 ,  G01N35/08 ,  G01N2035/00752 ,  Y10T436/11

Abstract: Provided are an automatic analyzing method and apparatus for a microfludic system. The apparatus includes means for recognizing a disc or chip in the microfludic system, performs an operation and detection by automatically loading a DB according to a recognized type, and perform quantitation of a test.

Abstract translation: 本发明提供一种微流体系统的自动分析方法和装置。 该装置包括用于识别微流体系统中的盘或芯片的装置，通过根据识别的类型自动加载数据库进行操作和检测，并执行测试的定量。

公开(公告)号：US20090322828A1

公开(公告)日：2009-12-31

申请号：US12552567

申请日：2009-09-02

Applicant: Su-hyeon KIM ,  Jeong-gun LEE ,  Jong-suk CHUNG ,  Hye-jung CHO ,  Nam HUH ,  In-seok KANG ,  Beom-seok LEE

Inventor： Su-hyeon KIM ,  Jeong-gun LEE ,  Jong-suk CHUNG ,  Hye-jung CHO ,  Nam HUH ,  In-seok KANG ,  Beom-seok LEE

IPC: B41J2/06

CPC classification number: B41J2/06 ,  B01J19/0046 ,  B01J2219/00364 ,  B01J2219/00497 ,  B01J2219/00527 ,  B01J2219/00585 ,  B01J2219/00596 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00637 ,  B01J2219/00659 ,  B01J2219/00677 ,  B01J2219/00689 ,  B01J2219/00691 ,  B01J2219/00693 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00743 ,  B41J2/04535 ,  B41J2/0456 ,  B41J2/04576

Abstract: A device for printing a droplet onto a substrate includes: a droplet generating member which is needle-shaped and comprises a receiving portion disposed vertically to receive a solution, and a discharge hole connected to the receiving portion and formed on a bottom of the receiving portion so that the solution can be discharged from the receiving portion; a substrate disposed below the droplet generating member, the substrate includes a target portion to which the droplet discharged from the discharge hole of the droplet generating member is dropped and attached; a voltage applier applying a voltage to the droplet so that the droplet can be dropped onto the target portion of the substrate; a volume measuring unit measuring the volume of the droplet; and a droplet control unit maintaining the volume of the droplet at a predetermined level based on the measured volume of the droplet.

Abstract translation: 用于将液滴印刷到基板上的装置包括：液滴产生构件，其为针状并且包括垂直设置以接收溶液的接收部分，以及连接到接收部分并形成在接收部分的底部上的排出孔 使得溶液可以从接收部分排出; 设置在所述液滴产生部件下方的基板，所述基板包括从所述液滴产生部件的排出孔排出的液滴滴落并附着到的目标部分; 电压施加器，向所述液滴施加电压，使得所述液滴能够落到所述基板的所述目标部分上; 体积测量单元，测量液滴的体积; 以及液滴控制单元，其基于所测量的液滴体积将液滴的体积保持在预定水平。

公开(公告)号：US20080014122A1

公开(公告)日：2008-01-17

申请号：US11560890

申请日：2006-11-17

Applicant: Su-hyeon KIM ,  Jeong-gun LEE

Inventor： Su-hyeon KIM ,  Jeong-gun LEE

IPC: B01L3/00

CPC classification number: C12M47/06 ,  B01L3/5027 ,  C12N1/066 ,  C12N13/00 ,  G01N1/44 ,  Y10T137/2496 ,  Y10T436/25

Abstract: A cell lysis device for lysing cells or viruses, comprising a cell lysis tube having a sample inlet; a pump connected to the cell lysis tube for transferring a sample into the tube; a sealing unit for reversibly sealing a specific region of the tube; and a laser source for generating a laser is provide. Further, a method of lysing cells or viruses using the cell lysis device is provide. The method comprises introducing a sample containing cells or viruses and optionally magnetic beads to the cell lysis tube through the sample inlet; transferring the sample to a specific region in the cell lysis tube by means of the pump; temporarily sealing the region of the cell lysis tube where the sample is placed with the sealing unit; irradiating the sample with the laser; removing the sealing unit from the cell lysis tube; and discharging the sample from the cell lysis tube by means of the pump.

Abstract translation: 一种用于裂解细胞或病毒的细胞裂解装置，包括具有样品入口的细胞裂解管; 连接到细胞裂解管的泵，用于将样品转移到管中; 用于可逆地密封管的特定区域的密封单元; 并提供用于产生激光的激光源。 此外，提供使用细胞裂解装置裂解细胞或病毒的方法。 该方法包括通过样品入口将含有细胞或病毒的样品和任选的磁珠引入细胞裂解管; 通过泵将样品转移到细胞裂解管中的特定区域; 用密封单元临时密封放置样品的细胞溶解管的区域; 用激光照射样品; 从细胞裂解管中取出密封单元; 并通过泵从细胞裂解管中排出样品。

公开(公告)号：US20090021741A1

公开(公告)日：2009-01-22

申请号：US11972266

申请日：2008-01-10

Applicant: Su-hyeon KIM ,  Jeong-gun LEE

Inventor： Su-hyeon KIM ,  Jeong-gun LEE

IPC: G01N21/00

CPC classification number: G01N21/07 ,  G01N21/253 ,  G01N21/276 ,  G01N21/314

Abstract: Provided are an optical detection apparatus, a microfluidic system including the same, and an optical detection method. The optical detection apparatus including: at least one light emission unit which emits light of a predetermined wavelength band; at least one light receiving unit which is disposed such that the light receiving unit receives the light emitted from the light emission unit and generates an electrical signal according to the intensity of the light received, wherein the number of light receiving units is the same as the number of light emission units; a rotation operating unit which rotates a disk-type microfluidic apparatus comprising at least one detection chamber in which a sample is to be loaded such that the detection chamber is disposed in a light pathway between the light emission unit and the light receiving unit; and a processor which measures a property of the sample contained in the detection chamber using the electrical signal generated by the light receiving unit.

Abstract translation: 提供了一种光学检测装置，包括该光学检测装置的微流体系统和光学检测方法。 所述光学检测装置包括：发射预定波长带的光的至少一个发光单元; 至少一个光接收单元，其设置成使得所述光接收单元接收从所述发光单元发射的光并根据所接收的光的强度产生电信号，其中所述光接收单元的数量与 发光单位数量; 旋转操作单元，其使包括至少一个检测室的盘式微流体装置旋转，其中样品将被装载，使得检测室设置在发光单元和光接收单元之间的光通路中; 以及处理器，其使用由光接收单元生成的电信号来测量包含在检测室中的样品的性质。

公开(公告)号：US20120225457A1

公开(公告)日：2012-09-06

申请号：US13294596

申请日：2011-11-11

Applicant: June-young LEE ,  Jwa-min NAM ,  Su-hyeon KIM ,  Woo-chang LEE ,  Jae-young KIM ,  Joo-won RHEE ,  Young-kwang LEE ,  Dong-kwon LIM ,  Jae-ho HWANG

Inventor： June-young LEE ,  Jwa-min NAM ,  Su-hyeon KIM ,  Woo-chang LEE ,  Jae-young KIM ,  Joo-won RHEE ,  Young-kwang LEE ,  Dong-kwon LIM ,  Jae-ho HWANG

IPC: C12P19/34 ,  B32B5/16 ,  C07H21/00 ,  B82Y99/00

CPC classification number: C07H21/00 ,  B82Y15/00 ,  C12P19/34 ,  Y10T428/2991

Abstract: A nanoparticle-nucleic acid complex and a method of linearizing a target nucleic acid by using the nanoparticle-nucleic acid complex are disclosed. By using the nanoparticle-nucleic acid complex and the method, nucleotide sequence analysis and mapping of a target nucleic acid may be efficiently performed.

Abstract translation: 公开了纳米颗粒 - 核酸复合物和通过使用纳米颗粒 - 核酸复合物线性化靶核酸的方法。 通过使用纳米粒子 - 核酸复合物和该方法，可以有效地进行目标核酸的核苷酸序列分析和作图。

公开(公告)号：US20100204065A1

公开(公告)日：2010-08-12

申请号：US12762596

申请日：2010-04-19

Applicant: Su-hyeon KIM ,  In-ho LEE ,  Jun-hong MIN

Inventor： Su-hyeon KIM ,  In-ho LEE ,  Jun-hong MIN

IPC: C40B50/14

CPC classification number: B82Y30/00 ,  B01J19/0046 ,  B01J2219/00369 ,  B01J2219/00432 ,  B01J2219/005 ,  B01J2219/0052 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00657 ,  B01J2219/00673 ,  B01J2219/00677 ,  B01J2219/00711 ,  B01J2219/00722 ,  B01J2219/00725 ,  B01J2219/00729 ,  B01J2219/00743

Abstract: A microarray including hydrogel and a plurality of probes which are immobilized in discrete regions of the hydrogel, and a method of preparing the same are provided. When using the microarray and method, a solid substrate is not required and many biomolecules can be immobilized in a small volume, thereby obtaining high sensitivity. Since gel can be cut to obtain many pieces, many microarrays can be prepared at once.

Abstract translation: 提供了包含水凝胶和固定在水凝胶的离散区域中的多个探针的微阵列及其制备方法。 当使用微阵列和方法时，不需要固体底物，并且许多生物分子可以以小体积固定，从而获得高灵敏度。 由于可以切割凝胶以获得许多片，因此可以一次制备许多微阵列。

公开(公告)号：US20120220473A1

公开(公告)日：2012-08-30

申请号：US13303813

申请日：2011-11-23

Applicant: Tae-jin AHN ,  Su-hyeon KIM ,  Kyoung-gu WOO

Inventor： Tae-jin AHN ,  Su-hyeon KIM ,  Kyoung-gu WOO

IPC: C40B30/04 ,  G06F19/00

CPC classification number: G06F19/16

Abstract: An efficient and accurate method of identifying a target biomolecule in a sample by using target molecule-probe binding frequencies is disclosed.

Abstract translation: 公开了通过使用靶分子 - 探针结合频率来鉴定样品中的靶生物分子的有效和准确的方法。

公开(公告)号：US20120064510A1

公开(公告)日：2012-03-15

申请号：US13224540

申请日：2011-09-02

Applicant: Joo-won RHEE ,  Su-hyeon KIM

Inventor： Joo-won RHEE ,  Su-hyeon KIM

IPC: G01N33/53 ,  G01N21/64 ,  G01N21/75 ,  C12Q1/70 ,  C12Q1/68

CPC classification number: C12Q1/6827 ,  G01N21/6428 ,  G01N33/5308 ,  G01N33/542 ,  G01N2021/6441 ,  C12Q2522/101 ,  C12Q2563/107 ,  C12Q2563/119

Abstract: A kit including a target sequence-binding protein and a method of detecting a target nucleic acid by using the kit that may ensure efficient detection of the target nucleic acid in a biological sample are disclosed.

Abstract translation: 公开了一种试剂盒，其包括靶序列结合蛋白和通过使用试剂盒检测靶核酸的方法，所述试剂盒可以确保生物样品中靶核酸的有效检测。

公开(公告)号：US20110046019A1

公开(公告)日：2011-02-24

申请号：US12702381

申请日：2010-02-09

Applicant: Su-hyeon KIM

Inventor： Su-hyeon KIM

IPC: C40B50/18 ,  C40B60/14

CPC classification number: G01N33/54353

Abstract: A method for immobilizing a target material on a substrate includes applying a voltage across a first solution including the target material in contact with a surface of a film including a pore disposed therein, a second solution in contact with an opposite surface of the film, and a substrate disposed facing the opposite surface of the film, moving the target material from the first solution, through the pore of the film into the second solution, and immobilizing the target material on the substrate.

Abstract translation: 将目标材料固定在基板上的方法包括在包括目标材料的第一溶液上施加电压，所述第一溶液与包括设置在其中的孔的膜的表面接触，与膜的相对表面接触的第二溶液，以及 面向所述膜的相对表面的基板，将所述目标材料从所述第一溶液移动通过所述膜的孔进入所述第二溶液，并将所述靶材固定在所述基板上。