公开(公告)号：EP0770210A1

公开(公告)日：1997-05-02

申请号：EP95924489.0

申请日：1995-07-14

Applicant: TECHNOBIOCHIP

Inventor： SARTORE, Marco ,  ADAMI, Manuela ,  NICOLINI, Claudio ,  TROITSKY, Vladimir

IPC: B05D1 ,  G01N27 ,  G01N33

CPC classification number: B82Y30/00 ,  B05D1/204 ,  B05D1/206 ,  B82Y40/00 ,  G01N29/036 ,  G01N33/54373 ,  G01N2291/0257

Abstract: A gravimetric biosensor based on two quartz resonators with immobilised layers of antibodies was developed. The active oscillator is covered by antibodies specific to a given antigen, while the reference oscillator is covered by antibodies non-specific to the antigen, in order to compensate the physical absorption effects. In a second aspect the invention provides a method and instrument for depositing films of alternating monomolecular layers, which can contain monolayers of surfactant molecules and monolayers of soluble adsorbed compounds. The first and second aspect in combination can provide a biosensor having alternating monomolecualr layers with at least one layer being biosensitive, e.g. immunosensitive.

公开(公告)号：EP0730760A1

公开(公告)日：1996-09-11

申请号：EP95901567.0

申请日：1994-11-25

Applicant: TECHNOBIOCHIP

Inventor： SARTORE, Marco ,  ADAMI, Manuela ,  NICOLINI, Claudio ,  FANIGLIULO, Antonio

IPC: C12Q1 ,  G01N33 ,  G05D23

CPC classification number: G01N33/54373 ,  C12Q1/001 ,  G05D23/1919 ,  G05D23/20

Abstract: The present invention relates generally to a potentiometric alternating biosensor system (PAB) which can use a silicon based light-addressable transducer to measure parameters such as pH and redox potential. A first aspect relates to temperature control of a PAB system. A second aspect relates to a biosensor for measuring pH and/or redox potential variations in enzymic reactions associated with cells. A third aspect relates to a silicon transducer redox potential biosensor having deposited thereon a metal layer over at least a portion of the surface, and its use in a PAB to monitor enzymes catalyzing redox reactions. A fourth aspect relates to a 2D PAB system capable of simultaneous pH and redox potential measurement. A fifth aspect relates to use of a PAB system for measurement of extracellular acidification to monitor cell metabolism and its use for testing anti-cancer activity/toxicity of compounds on cells. A sixth aspect relates to a PAB system capable of acting as an immunosensor in an immunoassay by virtue of the application to the transducer of an immobilized monolayer of an enzyme.