公开(公告)号：WO2013144359A3

公开(公告)日：2013-12-05

申请号：PCT/EP2013056846

申请日：2013-04-01

Applicant: TECHNOPATH DISTRIB LTD

Inventor： MOZOLOWSKI GUY ,  HYNES JAMES

IPC: G01N21/64

CPC classification number: G01N21/64 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2201/06113 ,  G01N2201/062 ,  G01N2201/0644

Abstract: A fluorescence microtitre plate reader comprises a plate reader body (2) having a base (3), top (5) and sidewalls (4), the top of the plate reader comprising at least one seat (6) dimensioned for receipt of a microtitre plate (7), the at least one seat defining an aperture (8) in the top of the plate that substantially corresponds to a base of the plate. The plate reader also includes means for illuminating wells of a microtitre plate with light from beneath the plate having an excitation wavelength, and a camera (10) and lens (11) assembly for capturing light emitted by the wells of the microtitre plate and generating a digital image, in which regions of light intensity of the image correspond to wells of the microtitre plate. An optical system is disposed within the plate reader body for expanding the distance to image plane of light, the optical system comprising a first mirror (15) disposed to receive light from the wells of the microtitre plate and reflect the light to second mirror (16) which is disposed to reflect incident light to the camera lens. The means for illumining the wells of the microtitre plate comprises a ring light (12) that embraces the camera and lens assembly.

Abstract translation: 荧光微量滴定板读取器包括具有基底（3），顶部（5）和侧壁（4）的平板读取器主体（2），所述读板器的顶部包括至少一个座（6），所述座（6）的尺寸适于接收微滴 所述板（7），所述至少一个座在所述板的顶部限定基本对应于所述板的基部的孔（8）。 读板器还包括用于用具有激发波长的板下方的光照射微量滴定板的孔的装置，以及用于捕获由微量滴定板的孔发射的光的照相机（10）和透镜（11）组件，并产生 数字图像，其中图像的光强度区域对应于微量滴定板的孔。 光学系统设置在板读取器本体内，用于扩大与光的像平面的距离，该光学系统包括设置成接收来自微量滴定板的孔的光的第一反射镜（15），并将光反射到第二反射镜 ），其被设置成将入射光反射到相机镜头。 用于照亮微量滴定板的孔的装置包括围绕相机和透镜组件的环形光（12）。