公开(公告)号：US20160356723A1

公开(公告)日：2016-12-08

申请号：US15114692

申请日：2015-01-27

Applicant: NATIONAL UNIVERSITY OF SINGAPORE ,  THE HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Bin Liu ,  Jinjun Shao ,  Youyong Yuan ,  Jing Liang ,  Benzhong Tang ,  Zhegang Song ,  Yilong Chen ,  Tsz Kin Kwok

IPC: G01N21/77 ,  C07K7/52 ,  A61K49/00 ,  A61K49/14 ,  G01N33/50 ,  A61K47/48 ,  C07D213/38 ,  C07F9/12 ,  C07K1/13 ,  C07K7/06 ,  A61K33/24

CPC classification number: G01N21/77 ,  A61K33/24 ,  A61K47/545 ,  A61K47/62 ,  A61K49/0056 ,  A61K49/146 ,  C07D213/38 ,  C07F7/0812 ,  C07F7/0896 ,  C07F9/12 ,  C07F15/0086 ,  C07F15/0093 ,  C07K1/13 ,  C07K5/1021 ,  C07K5/123 ,  C07K7/06 ,  C07K7/08 ,  C07K7/52 ,  G01N33/5008 ,  G01N33/542 ,  G01N33/582 ,  G01N2021/7786 ,  G01N2333/47 ,  G01N2333/916

Abstract: The present invention is drawn toward luminogens and chemical compositions comprising a target recognition motif, a hydrophilic moiety, a linking moiety, and at least one luminogen. Additionally presented are methods of: assessing the conversion of a prodrug into its active form, assessing the therapeutic efficacy of a prodrug, detecting glutathione in a biological sample, detecting alkaline phosphatase in a sample, and conducting fluorescence imaging or magnetic resonance imaging with the use of luminogen-containing compositions.

Abstract translation: 本发明涉及发光体和包含目标识别基序，亲水部分，连接部分和至少一种发光体的化学组合物。 另外提出的方法是：评估前药转化为其活性形式，评估前药的治疗功效，检测生物样品中的谷胱甘肽，检测样品中的碱性磷酸酶，以及使用荧光成像或磁共振成像 的含发光剂的组合物。

公开(公告)号：US10935552B2

公开(公告)日：2021-03-02

申请号：US15737187

申请日：2016-06-24

Applicant: THE HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Benzhong Tang ,  Engui Zhao ,  Chen Gui ,  Meijuan Jiang ,  Haiqin Deng ,  Wai Tung Leung ,  Zhiming Wang ,  Yueyue Zhao ,  Tsz Kin Kwok ,  Lucia Viglianti ,  Ni Xie

IPC: C09K11/06 ,  G01N33/58 ,  C08G65/00 ,  A61K49/00 ,  A61K41/00 ,  A61K31/4706 ,  A61K31/47 ,  A61K33/22 ,  A61K31/5375 ,  A61K31/695 ,  C09B23/04 ,  C09B26/02 ,  C09B57/00

Abstract: The present subject matter relates to ATE luminogens for visualization and treatment of cancer, particularly AIE luminogenic probes for cancer cell visualization and discrimination, lysosome-targeting AIEgens for imaging and autophagy visualization, highly fluorescent AIE-active theranostic agents for monitoring drug distribution and having anti-tumor activity to specific cancer cells, probes comprising AIE luminogens for cancer cell imaging and staining, AIE luminogens having clusteroluminogenic features and applications thereof, and methods of preparing thereof.

公开(公告)号：US11578265B2

公开(公告)日：2023-02-14

申请号：US16319436

申请日：2017-07-21

Applicant: THE HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Benzhong Tang ,  Xiujuan Shi ,  Yee Yung Yu ,  Tsz Kin Kwok ,  Zheng Zhao ,  Pengfei Zhang ,  Huifang Su

IPC: C09K11/06 ,  C07D221/14 ,  C07D213/57 ,  C07D401/12 ,  C07D285/14 ,  A61N5/06 ,  G01N33/533 ,  G01N33/58 ,  C07C211/43

Abstract: The present subject matter is directed to a luminogen exhibiting aggregation induced emission, wherein T1, T2, and T3 comprise one or more polyynes as a conjugated bridge. The present subject matter is also directed to an AIEgen comprising a hydrophilic pyridium group as a strong electron-withdrawing group; a piperazine group as an electron-donating group; and a α-Cyanostilbene; wherein the AIEgen exhibits aggregation induced emission. The present subject matter is directed to a method of synthesizing an AIEgen and is further directed to a method of labeling comprising incubating a subject having cells with a conjugate formed by conjugating an AIEgen with an antibody; and selectively labeling desired cells by turn-on imaging, wherein labeling occurs when the desired cells are selectively stained by fluorescent emission of the AIEgen upon degradation of the antibody after cellular internalization of the conjugate through endocytosis.

公开(公告)号：US09618453B2

公开(公告)日：2017-04-11

申请号：US13899070

申请日：2013-05-21

Applicant: THE HONG KONG UNIVERSITY OF SCIENCE AND TECHNOLOGY

Inventor： Benzhong Tang ,  Ka Ming Ng ,  Qian Luo ,  Yong Yu ,  Yuning Hong ,  Jianzhao Liu ,  Sijie Chen ,  Wing Yip Lam ,  Zhengke Wang ,  Wei Qin ,  Tsz Kin Kwok

IPC: C12Q1/04 ,  G01N21/64 ,  B82Y15/00 ,  G01N1/30 ,  C07F7/08

CPC classification number: G01N21/6486 ,  B82Y15/00 ,  C07F7/0805 ,  C07F7/0816 ,  G01N1/30 ,  G01N21/6428 ,  G01N21/6458 ,  G01N2021/6439

Abstract: Provided herein are fluorescent bioprobes comprising fluorogens that exhibit aggregation-induced emission (AIE) labeled on biomolecules. The present subject matter relates to a fluorescent bioprobe comprising one or more fluorogen labeled on chitosan. The present subject matter is also directed to methods of preparing the fluorescent bioprobes, methods of labeling and detecting DNA and/or proteins with the fluorescent bioprobe, and methods of cell imaging including live cell tracking.