公开(公告)号：JPH10165200A

公开(公告)日：1998-06-23

申请号：JP34058196

申请日：1996-12-05

Applicant: TOA ELECTRONICS

Inventor： HAKETA YASUSHI ,  KOBAYASHI KANEO

IPC: G01N21/78 ,  C12Q1/66

Abstract: PROBLEM TO BE SOLVED: To provide a method for assaying microbial ATP, capable of efficiently and accurately conducting a microbial assay for a sample rich in free ATP in a short time. SOLUTION: This method for microbial ATP assay is characterized by that an ATP-decomposing reagent is added to a sample rich in free ATP not derived from microorganism to decompose the free ATP in the sample followed by adding an ATP-extractive reagent to the resultant sample to extract ATP from the microorganisms. At this time, simultaneously, the extractive reagent deactivates the ATP-decomposing reagent added to the sample previously, and subsequently, a luminescent reagent containing a reagent suppressing the inhibition of an enzymatic reaction is brought into contact with the resultant sample, and the light generated by biological chemiluminescent reaction is assayed with a relevant device.

公开(公告)号：JPH07181161A

公开(公告)日：1995-07-21

申请号：JP34658793

申请日：1993-12-22

Applicant: TOA ELECTRONICS

Inventor： SHINNO TETSUHISA ,  OGAWA NOBUHIRO ,  UENO TADAAKI ,  KOBAYASHI KANEO

IPC: G01N27/26

Abstract: PURPOSE:To obtain the concentration meter which is operated stably in an automatic operation and whose reliability is enhanced by a method wherein the difference between an electromotive force in a calibrating operation at a low concentration and that in a previous calibrating operation, a generated electromotive force per pX in a calibrating operation, the stability of an electromotive force in a calibrating operation at a highconcentration and the responsivity in the calibrating operation at the high concentration are established as a rule and a fuzzy operation is performed. CONSTITUTION:Electric signals from individual electrodes 2, 4, 6 in a measuring cell 8 are amplified by an analog amplifier, they are converted into digital signals by an A/D converter 100, and the digital signals are input to a control part 102 provided with a microcomputer. The control part 102 controls a driver circuit 104, and it performs a fuzzy operation in order to obtain the sensitivity of the electrodes, the date and time of a calibrating operation and the like. Then, four elements, i.e., the difference between an electromotive force in a calibrating operation at a low concentration and that in a previous calibrating operation, a generated electromotive force per pX in a calibrating operation, the stability of an electromotive force in a calibrating operation at a high concentration and the responsivity of the calibrating operation at the high concentration, are established as a rule, a fuzzy operation is performed, the present sensitivity of the electrodes is found, a period of time during which reliable data can be measured on the basis of the sensitivity is estimated, and the date and time of a next calibrating operation is set.

公开(公告)号：JPH1189592A

公开(公告)日：1999-04-06

申请号：JP28284597

申请日：1997-09-30

Applicant: TOA ELECTRONICS

Inventor： HAKETA YASUSHI ,  KOBAYASHI KANEO

IPC: G01N21/76 ,  C12Q1/06 ,  C12Q1/66

Abstract: PROBLEM TO BE SOLVED: To provide a mensuration of ATP of microorganisms enabling to accurately measure the ATP of microorganism of a sample in biochemiluminescence by decomposing only free ATP in the sample containing free ATP in plenty such as foods, etc., utilizing ATP catabolic enzyme. SOLUTION: The microorganism contained in the sample is collected on a filter maternal by filtering sample containing the ATP decomposing reagent after decomposing free ATP in the sample containing microorganism by adding ATP decomposing reagent. The sample solution dissolving ATP decomposing reagent adhering on filtering material and the microorganism is removed by washing the filtering material with aseptic cleaning fluid. The ATP is extracted from the cells of the microorganism by applying an extracting reagent to the microorganism collected on the filtering material and the concentration of the extracted ATP of the microorganism is measured in biochemiluminescence using luciferase and luciferin.