Abstract:
PROBLEM TO BE SOLVED: To provide a method for increasing optical response marking or its S/N ratio of analytical chemistry sensor arrangement for detection of gaseous and liquid analytes. SOLUTION: In this method, analytical chemistry system based on microsphere is disclosed, wherein self-encoding microsphere having independent characteristic optical response marking to specific target analyte is mixed together, while maintaining capability for identifying each type and position in random distribution of these great many sensors on the sensor arrangement using encoding system of optical response. For each single sensor arrangement, its combined signal substantially is improved for sensor detection limit, response time and signal/noise ratio. COPYRIGHT: (C)2009,JPO&INPIT
Abstract:
PROBLEM TO BE SOLVED: To provide a new method of amplifying signals generated from an enzyme reaction. SOLUTION: A microsphere group including a plurality of identical microsphere partial groups including coupled enzymes is supplied, an optical fiber bundle is supplied to the microsphere group such that the microsphere is optically coupled to independent fibers or fiber groups of the optical fiber bundle, an analyte is supplied to the microsphere group, hence optical signals are generated, detected, and combined, and amplified signals from the enzyme reaction are generated, thus amplifying signals generated from the enzyme reaction. COPYRIGHT: (C)2011,JPO&INPIT
Abstract:
A microsphere-based analytic chemistry system is disclosed in which self- encoding microspheres having distinct characteristic optical response signatures to specific target analytes may be mixed together while the abili ty is retained to identify the sensor type and location of each sensor in a random dispersion of large numbers of such sensors in a sensor array using a n optically interrogatable encoding scheme. An optical fiber bundle sensor is also disclosed in which individual microsphere sensors are disposed in microwells at a distal end of the fiber bundle and are optically coupled to discrete fibers or groups of fibers within the bundle. The identities of the individual sensors in the array are self-encoded by exposing the array to a reference analyte while illuminating the array with excitation light energy. A single sensor array may carry thousands of discrete sensing elements whose combined signal provides for substantial improvements in sensor detection limits, response times and signal-to-noise ratios.
Abstract:
A combinatorial synthesis method for fabricating unique families of discrete copolymer sensors and copolymer gradient sensors is provided. The method employs combinatorial copolymer synthesis of discrete monomer or oligomer combinations as well as spatially-varying combinations for generating large numbers of analyte-discriminating sensors from a limited selection of initial monomer and oligomer compositions. The method can be applied to either analyte-specific sensors or sensor arrays or semi-selective sensors and cross-reactive sensor arrays which employ virtually any known physicochemical transduction mechanism for detecting analytes. Since the analyte response characteristics of such copolymer sensors are not limited to a linear proportional ratio of the monomer or oligomer combinations employed, the resulting copolymer sensors provide for increased diversity in sensor and sensor array response characteristics for discriminating between a variety of materials and for detecting and identifying analytes in fluid samples.
Abstract:
A microsphere-based analytic chemistry system is disclosed in which self-encoding microspheres having distinct characteristic optical response signatures to specific target analytes may be mixed together while the ability is retained to identify the sensor type and location of each sensor in a random dispersion of large numbers of such sensors in a sensor array using an optically interrogatable encoding scheme. An optical fiber bundle sensor is also disclosed in which individual microsphere sensors are disposed in microwells at a distal end of the fiber bundle and are optically coupled to discrete fibers or groups of fibers within the bundle. The identities of the individual sensors in the array are self-encoded by exposing the array to a reference analyte while illuminating the array with excitation light energy. A single sensor array may carry thousands of discrete sensing elements whose combined signal provides for substantial improvements in sensor detection limits, response and signal-to-noise ratios.
Abstract:
Un método para analizar datos de señal obtenidos a partir de una matriz para la unión de un analito diana, comprendiendo dicho método: a) proporcionar una matriz que comprende una primera subpoblación y una segunda subpoblación de cuentas distribuidas sobre una superficie de un sustrato, comprendiendo dichas cuentas sondas de ácido nucleico, en donde las cuentas en dicha primera subpoblación son diferentes de las cuentas en dicha segunda subpoblación y en donde cada una de la primera y la segunda subpoblaciones comprende una pluralidad de cuentas que comprenden sondas de ácido nucleico idénticas; b) proporcionar a dicha matriz un analito diana, en donde dicho analito diana es un ácido nucleico y se une a la primera subpoblación de elementos sensores y/o a la segunda población de elementos sensores; c) obtener señales de respuestas ópticas de una pluralidad de cuentas dentro de dicha primera subpoblación y/o dentro de dicha segunda subpoblación; y d) analizar estadísticamente las señales de respuesta obtenidas a partir de dicha primera subpoblación de cuentas y/o de dicha segunda subpoblación de cuentas, para hacer una estimación cuantitativa de la confianza acerca de los datos.