公开(公告)号：US12188003B1

公开(公告)日：2025-01-07

申请号：US18374098

申请日：2023-09-28

Applicant: Trisk Bio Ltd.

Inventor： Gergo Bohner ,  Adam Luke Hiles ,  Samuel Isidor Jones ,  Kevin Lynagh ,  Ryan Olf ,  Gabor Pap ,  Spencer Ryan Wilson ,  Thomas Felix Martin Cummings

IPC: C12M1/00 ,  C12M1/34

Abstract: Provided herein is a method for processing a liquid sample, utilizing a first component including an interface side and at least 3 sample containers or tanks; and a second component including a connector manifold. The method may include the step of reversibly connecting a reagent container, a filtration apparatus, and a chromatography apparatus to the manifold; reversibly connecting the first component to the connector manifold; pressurizing the first sample tank; transferring the sample into the filtration apparatus; passing the sample through a filter; transferring the sample into the second sample tank; transferring the sample into the chromatography apparatus; transferring the reagent into the chromatography apparatus; and transferring the sample to the third sample tank. Some or all transfers may be routed via the connector manifold and the interface side.

公开(公告)号：US11939565B1

公开(公告)日：2024-03-26

申请号：US18215393

申请日：2023-06-28

Applicant: Trisk Bio Ltd.

Inventor： Gergo Bohner ,  Adam Luke Hiles ,  Samuel Isidor Jones ,  Kevin Lynagh ,  Ryan Olf ,  Gabor Pap ,  Spencer Ryan Wilson ,  Thomas Felix Martin Cummings

IPC: C12M1/34 ,  C12M1/12 ,  C12M1/36

CPC classification number: C12M41/40 ,  C12M37/00 ,  C12M41/44 ,  C12M41/48

Abstract: Provided herein is a method for incubating living cells, in accordance with principles of the disclosure, may include the steps of: (a) providing a pressurized gas to a medium reservoir, the pressurized gas providing an impetus that moves a growth medium in the medium reservoir to a bioreactor chamber via a first incoming fluid line connecting the medium reservoir to the bioreactor chamber; (b) simultaneously or subsequently to step (a), providing a pressurized gas to a cell reservoir holding a suspension of the living cells, the pressurized gas providing an impetus that moves the suspension to the bioreactor chamber via a second incoming fluid line connecting the cell reservoir to the bioreactor chamber; and (c) incubating the growth medium and the living cells in the bioreactor chamber, under conditions compatible with cell viability.