公开(公告)号：US09476081B2

公开(公告)日：2016-10-25

申请号：US14451975

申请日：2014-08-05

Applicant: UCB PHARMA S.A.

Inventor： Katharine Lacy Cain ,  Shirley Jane Peters ,  Paul Edward Stephens

IPC: C12N15/63 ,  C12P21/00 ,  C07K14/47 ,  C07K16/00 ,  C07K16/18 ,  C07K16/40 ,  C12N9/02 ,  C12P21/02

CPC classification number: C12P21/00 ,  C07K14/47 ,  C07K16/00 ,  C07K16/18 ,  C07K16/40 ,  C12N9/0004 ,  C12P21/02 ,  Y02P20/52

Abstract: The present invention relates to a recombinant host cell, wherein the cell is modified to increase the expression levels of Ero1 and XBP1 relative to the expression levels of Ero1 and XBP1 in an unmodified cell. The present invention also relates to a method of producing a recombinant protein of interest comprising expressing the recombinant protein of interest in the recombinant host cell.

公开(公告)号：US20190031752A1

公开(公告)日：2019-01-31

申请号：US15898286

申请日：2018-02-16

Applicant: UCB PHARMA S.A.

Inventor： Paul Edward Stephens ,  Michael John Wright

IPC: C07K16/24

Abstract: This invention provides a method for obtaining a recombinant antibody with a desired function, comprising: (a) providing a population of anti-body-forming cells suspected of containing at least one cell capable of producing an antibody exhibiting the desired function; (b) generating one or more transcriptionally active recombinant linear polynucleotides from the antibody-forming cells obtained in step (a) wherein each transcriptionally active recombinant linear polynucleotide comprises a polynucleotide sequence encoding a variable domain of an antibody produced by an antibody-forming cell obtained in step (a) and one or more transcription regulatory elements; (c) expressing a recombinant antibody using one or more of the transcriptionally active recombinant linear polynucleotides generated in step (b); (d) screening the recombinant antibody produced by step (c) for the desired function; and (e) optionally repeating steps (b), (c) and (d) to identify a recombinant antibody exhibiting the desired function.

公开(公告)号：US20150025128A1

公开(公告)日：2015-01-22

申请号：US14451975

申请日：2014-08-05

Applicant: UCB PHARMA S.A.

Inventor： Katharine Lacy Cain ,  Shirley Jane Peters ,  Paul Edward Stephens

IPC: C12P21/00

CPC classification number: C12P21/00 ,  C07K14/47 ,  C07K16/00 ,  C07K16/18 ,  C07K16/40 ,  C12N9/0004 ,  C12P21/02 ,  Y02P20/52

Abstract: The present invention relates to a recombinant host cell, wherein the cell is modified to increase the expression levels of Ero1 and XBP1 relative to the expression levels of Ero1 and XBP1 in an unmodified cell. The present invention also relates to a method of producing a recombinant protein of interest comprising expressing the recombinant protein of interest in the recombinant host cell.

Abstract translation: 本发明涉及重组宿主细胞，其中修饰细胞以相对于未修饰的细胞中Ero1和XBP1的表达水平增加Ero1和XBP1的表达水平。 本发明还涉及生产重组蛋白质的方法，包括在重组宿主细胞中表达重组蛋白质。