公开(公告)号：EP2656066A4

公开(公告)日：2017-11-08

申请号：EP11850829

申请日：2011-12-21

Applicant: UNIV CALIFORNIA

Inventor： OZCAN AYDOGAN ,  ZHU HONGYING ,  MAVANDADI SAM

IPC: G01N21/64 ,  B01L3/00 ,  B01L9/00 ,  G01N15/14 ,  G01N33/18 ,  G02B7/00 ,  G02B7/02 ,  G02B13/00 ,  H04M1/02 ,  H04M1/21 ,  H04N5/225

CPC classification number: G01N21/6458 ,  B01L3/502715 ,  B01L9/527 ,  B01L2300/0654 ,  B01L2300/0816 ,  G01N15/1436 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/648 ,  G01N2015/144 ,  G01N2021/6467 ,  G01N2021/6482 ,  G01N2201/0221 ,  G01N2201/0693 ,  G02B7/006 ,  G02B7/02 ,  G02B13/0025 ,  H04M1/0254 ,  H04M1/21 ,  H04M2250/52 ,  H04N5/2254

公开(公告)号：WO2012088351A3

公开(公告)日：2012-11-15

申请号：PCT/US2011066647

申请日：2011-12-21

Applicant: UNIV CALIFORNIA ,  OZCAN AYDOGAN ,  ZHU HONGYING ,  MAVANDADI SAM

Inventor： OZCAN AYDOGAN ,  ZHU HONGYING ,  MAVANDADI SAM

IPC: G01N33/48 ,  C12N13/00 ,  C12Q1/02 ,  G01N33/52

CPC classification number: G01N21/6458 ,  B01L3/502715 ,  B01L9/527 ,  B01L2300/0654 ,  B01L2300/0816 ,  G01N15/1436 ,  G01N15/1459 ,  G01N15/1484 ,  G01N21/648 ,  G01N2015/144 ,  G01N2021/6467 ,  G01N2021/6482 ,  G01N2201/0221 ,  G01N2201/0693 ,  G02B7/006 ,  G02B7/02 ,  G02B13/0025 ,  H04M1/0254 ,  H04M1/21 ,  H04M2250/52 ,  H04N5/2254

Abstract: Wide-field fluorescent imaging on a mobile device having a camera is accomplished with a compact, light-weight and inexpensive optical components that are mechanically secured to the mobile device in a removable housing. Battery powered light-emitting diodes (LEDs) contained in the housing pump the sample of interest from the side using butt-coupling, where the pump light is guided within the microfluidic flow cell to uniformly excite the specimen. The fluorescent emission from the sample is then imaged using an additional lens that is positioned adjacent to the existing lens of the mobile device. A color filter is sufficient to create the dark-field background required for fluorescent imaging, without the need for expensive thin-film interference filters.

Abstract translation: 具有照相机的移动设备上的宽场荧光成像通过紧凑，重量轻且廉价的光学部件来实现，该组件机械地固定在移动设备中的可拆卸外壳中。 包含在壳体中的电池供电的发光二极管（LED）使用对接耦合从侧面泵送感兴趣的样品，其中泵浦光在微流体流动池内被引导以均匀地激发样品。 然后使用与移动设备的现有镜头相邻定位的附加镜头来成像来自样品的荧光发射。 滤色器足以产生荧光成像所需的暗场背景，而不需要昂贵的薄膜干涉滤光片。