中科微点-全球专利检索

  1. Login
  2. Sign Up

Search Results

2 records (took 0.019 seconds)

    MULTIMODAL ANALYSIS OF CIRCULATING TUMOR NUCLEIC ACID MOLECULES
    1.
    发明申请
    MULTIMODAL ANALYSIS OF CIRCULATING TUMOR NUCLEIC ACID MOLECULES 审中-公开

    公开(公告)号：WO2021253138A1

    公开(公告)日：2021-12-23

    申请号：PCT/CA2021/050842

    申请日：2021-06-18

    Applicant: UNIVERSITY HEALTH NETWORK

    Inventor： BRATMAN, Scott Victor ,  BURGENER, Justin Matthew ,  DINIZ DE CARVALHO, Daniel

    IPC: C12Q1/6809 ,  C12Q1/6886 ,  G16B20/00 ,  G16B30/00 ,  C12Q1/68

    Abstract: In an aspect, there is provided a method of detecting the presence of ctDNA from cancer cells in a subject comprising: (a) providing a sample of cell-free DNA from a subject; (b) subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; (c) optionally adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated, then further optionally denaturing the sample; (d) capturing cell-free methylated DNA using a binder selective for methylated polynucleotides; (e) sequencing the captured cell-free methylated DNA; (f) comparing the sequences of the captured cell-free methylated DNA to control cell-free methylated DNAs sequences from healthy and cancerous individuals; (g) identifying the presence of DNA from cancer cells if there is a statistically significant similarity between one or more sequences of the captured cell-free methylated DNA and cell-free methylated DNAs sequences from cancerous individuals; wherein in at least one of the capturing step, the comparing step or the identifying step, the subject cell-free methylated DNA is limited to a sub-population according to a fragment length metric.

Patent Agency Ranking